Search results for the GEO ID: GSE18752  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM465593 | GPL1261 |
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mouse natural helper cell 1
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unstimulated natural helper cells
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strain: C57BL6
tissue: Mesentery
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mouse natural helper cell, mLSK1
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| Sample_geo_accession | GSM465593
| | Sample_status | Public on Oct 28 2009
| | Sample_submission_date | Oct 27 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mesenteries separated from intestine and mesenteric lymph nodes were cut into small fragments with scissors and digested in Dulbecco's modified Eagle's Medium containing 2 mg/ml collagenase type I and 4% BSA. The supernatant was aspirated off after centrifugation to remove adipocytes. Finally, cells were suspended in Hank’s Balanced Salt Solution containing 10% FCS after filtration through 32 µm nylon mesh.
| | Sample_growth_protocol_ch1 | Thy-1+CD4- LTi cells were prepared from fetal liver cells as described previously (J. Immunol. 167, 2511-2521, 2001). c-Kit+α4β7+IL-7Rα+ fetal liver cells from day 13 embryos were cultured on TSt4 cells for 17 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 5 x 105 FALC c-Kit+Sca-1+ cells, LTi cells and DN2 cells after direct sorting into a vial containing ISOGEN LS (Nippon Gene, Tokyo, Japan). Total RNA of LTi cells was also extracted in ISOGEN LS. Total RNA was further purified using an RNeasy Micro Kit (QIAGEN, Tokyo, Japan) and amplified by Two-Cycle Target Labeling method (Affymetrix, Santa Clara, CA). Microarray processing was done by the Central Research Laboratory, Keio University School of Medicine.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, ver. 6, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operation Software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | kazuyo,,moro
| | Sample_contact_email | kazuyo@sc.itc.keio.ac.jp
| | Sample_contact_phone | +81-3-5363-3769
| | Sample_contact_laboratory | Koyasu
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio University school of medicine
| | Sample_contact_address | 35
| | Sample_contact_city | Sinjuku-ku Shinanomachi
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465593/suppl/GSM465593.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465593/suppl/GSM465593.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE18752
| | Sample_data_row_count | 45101
| | |
|
GSM465594 | GPL1261 |
|
mouse natural helper cell 2
|
unstimulated natural helper cells
|
strain: C57BL6
tissue: Mesentery
|
mouse natural helper cell, mLSK2
|
| Sample_geo_accession | GSM465594
| | Sample_status | Public on Oct 28 2009
| | Sample_submission_date | Oct 27 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mesenteries separated from intestine and mesenteric lymph nodes were cut into small fragments with scissors and digested in Dulbecco's modified Eagle's Medium containing 2 mg/ml collagenase type I and 4% BSA. The supernatant was aspirated off after centrifugation to remove adipocytes. Finally, cells were suspended in Hank’s Balanced Salt Solution containing 10% FCS after filtration through 32 µm nylon mesh.
| | Sample_growth_protocol_ch1 | Thy-1+CD4- LTi cells were prepared from fetal liver cells as described previously (J. Immunol. 167, 2511-2521, 2001). c-Kit+α4β7+IL-7Rα+ fetal liver cells from day 13 embryos were cultured on TSt4 cells for 17 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 5 x 105 FALC c-Kit+Sca-1+ cells, LTi cells and DN2 cells after direct sorting into a vial containing ISOGEN LS (Nippon Gene, Tokyo, Japan). Total RNA of LTi cells was also extracted in ISOGEN LS. Total RNA was further purified using an RNeasy Micro Kit (QIAGEN, Tokyo, Japan) and amplified by Two-Cycle Target Labeling method (Affymetrix, Santa Clara, CA). Microarray processing was done by the Central Research Laboratory, Keio University School of Medicine.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, ver. 6, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operation Software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | kazuyo,,moro
| | Sample_contact_email | kazuyo@sc.itc.keio.ac.jp
| | Sample_contact_phone | +81-3-5363-3769
| | Sample_contact_laboratory | Koyasu
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio University school of medicine
| | Sample_contact_address | 35
| | Sample_contact_city | Sinjuku-ku Shinanomachi
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465594/suppl/GSM465594.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465594/suppl/GSM465594.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE18752
| | Sample_data_row_count | 45101
| | |
|
GSM465595 | GPL1261 |
|
mouse thymus double negative 2 cell
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sorted DN2 cells
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strain: C57BL6
tissue: Thymus
|
mouse thymus double negatibe 2 cell, DN2
|
| Sample_geo_accession | GSM465595
| | Sample_status | Public on Oct 28 2009
| | Sample_submission_date | Oct 27 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mesenteries separated from intestine and mesenteric lymph nodes were cut into small fragments with scissors and digested in Dulbecco's modified Eagle's Medium containing 2 mg/ml collagenase type I and 4% BSA. The supernatant was aspirated off after centrifugation to remove adipocytes. Finally, cells were suspended in Hank’s Balanced Salt Solution containing 10% FCS after filtration through 32 µm nylon mesh.
| | Sample_growth_protocol_ch1 | Thy-1+CD4- LTi cells were prepared from fetal liver cells as described previously (J. Immunol. 167, 2511-2521, 2001). c-Kit+α4β7+IL-7Rα+ fetal liver cells from day 13 embryos were cultured on TSt4 cells for 17 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 5 x 105 FALC c-Kit+Sca-1+ cells, LTi cells and DN2 cells after direct sorting into a vial containing ISOGEN LS (Nippon Gene, Tokyo, Japan). Total RNA of LTi cells was also extracted in ISOGEN LS. Total RNA was further purified using an RNeasy Micro Kit (QIAGEN, Tokyo, Japan) and amplified by Two-Cycle Target Labeling method (Affymetrix, Santa Clara, CA). Microarray processing was done by the Central Research Laboratory, Keio University School of Medicine.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, ver. 6, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operation Software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | kazuyo,,moro
| | Sample_contact_email | kazuyo@sc.itc.keio.ac.jp
| | Sample_contact_phone | +81-3-5363-3769
| | Sample_contact_laboratory | Koyasu
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio University school of medicine
| | Sample_contact_address | 35
| | Sample_contact_city | Sinjuku-ku Shinanomachi
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465595/suppl/GSM465595.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465595/suppl/GSM465595.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE18752
| | Sample_data_row_count | 45101
| | |
|
GSM465596 | GPL1261 |
|
mouse lymphoid tissue inducer cell
|
cultured Lti cells
|
strain: C57BL6
tissue: Embryo, Fetal Liver (E13)
|
mouse lymphoid tissue inducer cell, LTi
|
| Sample_geo_accession | GSM465596
| | Sample_status | Public on Oct 28 2009
| | Sample_submission_date | Oct 27 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mesenteries separated from intestine and mesenteric lymph nodes were cut into small fragments with scissors and digested in Dulbecco's modified Eagle's Medium containing 2 mg/ml collagenase type I and 4% BSA. The supernatant was aspirated off after centrifugation to remove adipocytes. Finally, cells were suspended in Hank’s Balanced Salt Solution containing 10% FCS after filtration through 32 µm nylon mesh.
| | Sample_growth_protocol_ch1 | Thy-1+CD4- LTi cells were prepared from fetal liver cells as described previously (J. Immunol. 167, 2511-2521, 2001). c-Kit+α4β7+IL-7Rα+ fetal liver cells from day 13 embryos were cultured on TSt4 cells for 17 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 5 x 105 FALC c-Kit+Sca-1+ cells, LTi cells and DN2 cells after direct sorting into a vial containing ISOGEN LS (Nippon Gene, Tokyo, Japan). Total RNA of LTi cells was also extracted in ISOGEN LS. Total RNA was further purified using an RNeasy Micro Kit (QIAGEN, Tokyo, Japan) and amplified by Two-Cycle Target Labeling method (Affymetrix, Santa Clara, CA). Microarray processing was done by the Central Research Laboratory, Keio University School of Medicine.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, ver. 6, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operation Software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | kazuyo,,moro
| | Sample_contact_email | kazuyo@sc.itc.keio.ac.jp
| | Sample_contact_phone | +81-3-5363-3769
| | Sample_contact_laboratory | Koyasu
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio University school of medicine
| | Sample_contact_address | 35
| | Sample_contact_city | Sinjuku-ku Shinanomachi
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465596/suppl/GSM465596.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465596/suppl/GSM465596.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE18752
| | Sample_data_row_count | 45101
| | |
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