Result

Search results for the GEO ID: GSE18801

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Title

Source name

Description

Characteristics

GSM466341

GPL1261

Heart_control_rep1

Heart, left ventricle

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: control

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466341
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466341/suppl/GSM466341_WT_A.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466348

GPL1261

Heart_control_rep2

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: control

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466348
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466348/suppl/GSM466348_WT_B.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466349

GPL1261

Heart_control_rep3

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: control

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466349
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466349/suppl/GSM466349_WT_E.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466350

GPL1261

Heart_ISO_rep1

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: isoproterenol-induced cardiomyopathy

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466350
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466350/suppl/GSM466350_CTR_726.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466351

GPL1261

Heart_ISO_rep2

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: isoproterenol-induced cardiomyopathy

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466351
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466351/suppl/GSM466351_CTR_728.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466394

GPL1261

Heart_ISO_rep3

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: isoproterenol-induced cardiomyopathy

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466394
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466394/suppl/GSM466394_CTR_730.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466399

GPL1261

Heart_swim_rep1

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: exercise-induced cardiac hypertrophy

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466399
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466399/suppl/GSM466399_SND_1.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466400

GPL1261

Heart_swim_rep2

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: exercise-induced cardiac hypertrophy

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466400
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466400/suppl/GSM466400_SND_2.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

GSM466401

GPL1261

Heart_swim_rep3

Heart

sample source: Eight-week old male C57BL/6 mice, purchased from the Jackson Laboratory tissue: heart left ventricle protocol: exercise-induced cardiac hypertrophy

Study was performed to compare pathological versus physiological cardiac hypertrophy

Sample_geo_accession	GSM466401
Sample_status	Public on Oct 31 2009
Sample_submission_date	Oct 29 2009
Sample_last_update_date	Mar 24 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Garnering Innovation Lab at UTSW
Sample_treatment_protocol_ch1	Pathological cardiac hypertrophy was investigated using the isoproterenol-induced subacute myocardial injury model as previously described [28]. Briefly, animals were anesthetized with 1.5% isoflurane (Smiths Medical PM, Waukesha, WI) in 98.5% oxygen and a 1 cm incision made on the back of each animal between the shoulder blades. An Alzet 1007D micro-osmotic pump (DURECT Corporation, Cupertino, CA) containing isoproterenol (ISO, Sigma-Aldrich, St. Louis, MO), at 40 mg.kg-1.d-1, dissolved in 0.9% NaCl was inserted into the infrascapular subcutaneous tissue and the incision sutured. After 10 days of ISO administration, mice were sacrificed, and left ventricles were collected and processed for subsequent assays. Experimental physiological cardiac hypertrophy was induced via exercise, as previously described [29]. Briefly, mice were placed in buckets of pre-warmed water maintained at ~30 oC with low-watt heat lamps and allowed to swim for 90 minutes twice daily. At the beginning of the experiment, mice were acclimated to the exercise routine gradually, beginning with 10 minutes twice daily and increasing in increments of 10 minutes per day, until 90 minutes was obtained. After 8 weeks of swimming, mice were sacrificed and heart samples (left ventricles) collected and processed for each assay. Sedentary mice confined to cages served as negative controls.
Sample_growth_protocol_ch1	All mice were housed in the Animal facility at University of Texas Southwestern Medical Center (UTSW), Dallas, TX, in accordance with the standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996). All experimental procedures for this study were approved by the Institutional Animal Care and Use Committee at UTSW.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from left ventricles of experimental animals was isolated using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA) per manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation.
Sample_label_ch1	per manufacturer, in UTSW Microarray Core
Sample_label_protocol_ch1	per manufacturer, in UTSW Microarray Core
Sample_hyb_protocol	per manufacturer, in UTSW Microarray Core
Sample_scan_protocol	per manufacturer, in UTSW Microarray Core
Sample_data_processing	Data were analyzed using GeneSifter (VizX Labs, Seattle, WA) and Spotfire DecisionSite 9.0 (Spotfire, Inc., Somerville, MA). Data were normalized using robust-multi average (RMA) method, and signals for each group were averaged before performing Student’s t test with Benjamini and Hochberg correction and pairwise comparisons for sedentary mice versus mice that received ISO treatment or were exercised. Genes were considered as altered if the folds-change was at least 1.5 and adjusted p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Cristi,Lara,Galindo
Sample_contact_email	Cristi.Galindo@utsouthwestern.edu
Sample_contact_phone	214-648-1104
Sample_contact_fax	214-648-1445
Sample_contact_laboratory	Garnering Innovation
Sample_contact_department	McDermmot
Sample_contact_institute	University of Texas Southwestern Medical Center at Dallas
Sample_contact_address	2201 Inwood Dr.
Sample_contact_city	Dallas
Sample_contact_state	TX
Sample_contact_zip/postal_code	75395
Sample_contact_country	USA
Sample_contact_web_link	http://innovation.swmed.edu
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM466nnn/GSM466401/suppl/GSM466401_SND_3.CEL.gz
Sample_series_id	GSE18801
Sample_data_row_count	45101

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