Search results for the GEO ID: GSE18866  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM467634 | GPL570 |
|
EV_1
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: empty vector
|
Gene expression data from EV-transfected cells
|
| Sample_geo_accession | GSM467634
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467634/suppl/GSM467634.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467635 | GPL570 |
|
EV_2
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: empty vector
|
Gene expression data from EV-transfected cells
|
| Sample_geo_accession | GSM467635
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467635/suppl/GSM467635.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467636 | GPL570 |
|
EV_3
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: empty vector
|
Gene expression data from EV-transfected cells
|
| Sample_geo_accession | GSM467636
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467636/suppl/GSM467636.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467637 | GPL570 |
|
EV_4
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: empty vector
|
Gene expression data from EV-transfected cells
|
| Sample_geo_accession | GSM467637
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467637/suppl/GSM467637.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467638 | GPL570 |
|
EV_5
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: empty vector
|
Gene expression data from EV-transfected cells
|
| Sample_geo_accession | GSM467638
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467638/suppl/GSM467638.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467639 | GPL570 |
|
EV_6
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: empty vector
|
Gene expression data from EV-transfected cells
|
| Sample_geo_accession | GSM467639
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467639/suppl/GSM467639.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467640 | GPL570 |
|
miR_1
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: doxycyclin-inducible expression vector containing miR-15a/16-1
|
Gene expression data from miR-15a/16-1-transfected cells
|
| Sample_geo_accession | GSM467640
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467640/suppl/GSM467640.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467641 | GPL570 |
|
miR_2
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: doxycyclin-inducible expression vector containing miR-15a/16-1
|
Gene expression data from miR-15a/16-1-transfected cells
|
| Sample_geo_accession | GSM467641
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467641/suppl/GSM467641.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467642 | GPL570 |
|
miR_3
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: doxycyclin-inducible expression vector containing miR-15a/16-1
|
Gene expression data from miR-15a/16-1-transfected cells
|
| Sample_geo_accession | GSM467642
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467642/suppl/GSM467642.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467643 | GPL570 |
|
miR_4
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: doxycyclin-inducible expression vector containing miR-15a/16-1
|
Gene expression data from miR-15a/16-1-transfected cells
|
| Sample_geo_accession | GSM467643
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467643/suppl/GSM467643.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
|
GSM467644 | GPL570 |
|
miR_5
|
Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: doxycyclin-inducible expression vector containing miR-15a/16-1
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Gene expression data from miR-15a/16-1-transfected cells
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| Sample_geo_accession | GSM467644
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467644/suppl/GSM467644.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
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GSM467645 | GPL570 |
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miR_6
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Stably transfected 13q14-/- CLL-I83E95 cell line
|
tumor/tissue type: CLL cell line
cell line: CLL-I83E95
genome/variation: 13q14-\-
transfectant: doxycyclin-inducible expression vector containing miR-15a/16-1
|
Gene expression data from miR-15a/16-1-transfected cells
|
| Sample_geo_accession | GSM467645
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Nov 03 2009
| | Sample_last_update_date | Nov 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were harvested 48 hours after addition of doxycyclin that results in the induction of miR-expression.
| | Sample_growth_protocol_ch1 | CLL-I83U95 cells were cultured in 20% FCS-IMDM media at 0.5-1x10(6) cells/ml density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133_Plus2 Gene Expression Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulf,,Klein
| | Sample_contact_email | uk30@columbia.edu
| | Sample_contact_laboratory | Dalla-Favera
| | Sample_contact_department | Herbert Irving Comprehensive Cancer Center
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM467nnn/GSM467645/suppl/GSM467645.CEL.gz
| | Sample_series_id | GSE18866
| | Sample_data_row_count | 54613
| | |
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