Search results for the GEO ID: GSE18887 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM468220 | GPL96 |
|
Embryos at Carnegie Stage 9 (E20-21d), Replicate 1
|
Human embryos with Carnegie Stage 9 (E20-21d)
|
carnegie stage: S9
embryonic day: E20-21d
tissue: embryo
|
Human embryos with Carnegie Stage 9 (E20-21d), Replicate 1
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468220
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468220/suppl/GSM468220_S9_R1.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468221 | GPL96 |
|
Embryos at Carnegie Stage 9 (E20-21d), Replicate 2
|
Human embryos with Carnegie Stage 9 (E20-21d)
|
carnegie stage: S9
embryonic day: E20-21d
tissue: embryo
|
Human embryos with Carnegie Stage 9 (E20-21d), Replicate 2
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468221
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468221/suppl/GSM468221_S9_R2.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468222 | GPL96 |
|
Embryos at Carnegie Stage 9 (E20-21d), Replicate 3
|
Human embryos with Carnegie Stage 9 (E20-21d)
|
carnegie stage: S9
embryonic day: E20-21d
tissue: embryo
|
Human embryos with Carnegie Stage 9 (E20-21d), Replicate 3
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468222
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468222/suppl/GSM468222_S9_R3.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468223 | GPL96 |
|
Embryos at Carnegie Stage 10 (E22-23d), Replicate 1
|
Human embryos with Carnegie Stage 10 (E22-23d)
|
carnegie stage: S10
embryonic day: E22-23d
tissue: embryo
|
Human embryos with Carnegie Stage 10 (E22-23d), Replicate 1
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468223
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468223/suppl/GSM468223_S10_R1.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468224 | GPL96 |
|
Embryos at Carnegie Stage 10 (E22-23d), Replicate 2
|
Human embryos with Carnegie Stage 10 (E22-23d)
|
carnegie stage: S10
embryonic day: E22-23d
tissue: embryo
|
Human embryos with Carnegie Stage 10 (E22-23d), Replicate 2
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468224
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468224/suppl/GSM468224_S10_R2.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468225 | GPL96 |
|
Embryos at Carnegie Stage 10 (E22-23d), Replicate 3
|
Human embryos with Carnegie Stage 10 (E22-23d)
|
carnegie stage: S10
embryonic day: E22-23d
tissue: embryo
|
Human embryos with Carnegie Stage 10 (E22-23d), Replicate 3
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468225
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468225/suppl/GSM468225_S10_R3.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468226 | GPL96 |
|
Embryos at Carnegie Stage 11 (E24-25d), Replicate 1
|
Human embryos with Carnegie Stage 11 (E24-25d)
|
carnegie stage: S11
embryonic day: E24-25d
tissue: embryo
|
Human embryos with Carnegie Stage 11 (E24-25d), Replicate 1
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468226
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468226/suppl/GSM468226_S11_R1.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468227 | GPL96 |
|
Embryos at Carnegie Stage 11 (E24-25d), Replicate 2
|
Human embryos with Carnegie Stage 11 (E24-25d)
|
carnegie stage: S11
embryonic day: E24-25d
tissue: embryo
|
Human embryos with Carnegie Stage 11 (E24-25d), Replicate 2
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468227
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468227/suppl/GSM468227_S11_R2.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468228 | GPL96 |
|
Embryos at Carnegie Stage 11 (E24-25d), Replicate 3
|
Human embryos with Carnegie Stage 11 (E24-25d)
|
carnegie stage: S11
embryonic day: E24-25d
tissue: embryo
|
Human embryos with Carnegie Stage 11 (E24-25d), Replicate 3
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468228
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468228/suppl/GSM468228_S11_R3.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468229 | GPL96 |
|
Embryos at Carnegie Stage 12 (E26-27d), Replicate 1
|
Human embryos with Carnegie Stage 12 (E26-27d)
|
carnegie stage: S12
embryonic day: E26-27d
tissue: embryo
|
Human embryos with Carnegie Stage 12 (E26-27d), Replicate 1
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468229
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468229/suppl/GSM468229_S12_R1.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468230 | GPL96 |
|
Embryos at Carnegie Stage 12 (E26-27d), Replicate 2
|
Human embryos with Carnegie Stage 12 (E26-27d)
|
carnegie stage: S12
embryonic day: E26-27d
tissue: embryo
|
Human embryos with Carnegie Stage 12 (E26-27d), Replicate 2
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468230
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468230/suppl/GSM468230_S12_R2.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468231 | GPL96 |
|
Embryos at Carnegie Stage 12 (E26-27d), Replicate 3
|
Human embryos with Carnegie Stage 12 (E26-27d)
|
carnegie stage: S12
embryonic day: E26-27d
tissue: embryo
|
Human embryos with Carnegie Stage 12 (E26-27d), Replicate 3
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468231
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468231/suppl/GSM468231_S12_R3.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468232 | GPL96 |
|
Embryos at Carnegie Stage 13 (E28-30d), Replicate 1
|
Human embryos with Carnegie Stage 13 (E28-30d)
|
carnegie stage: S13
embryonic day: E28-30d
tissue: embryo
|
Human embryos with Carnegie Stage 13 (E28-30d), Replicate 1
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468232
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468232/suppl/GSM468232_S13_R1.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468233 | GPL96 |
|
Embryos at Carnegie Stage 13 (E28-30d), Replicate 2
|
Human embryos with Carnegie Stage 13 (E28-30d)
|
carnegie stage: S13
embryonic day: E28-30d
tissue: embryo
|
Human embryos with Carnegie Stage 13 (E28-30d), Replicate 2
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468233
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468233/suppl/GSM468233_S13_R2.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468234 | GPL96 |
|
Embryos at Carnegie Stage 13 (E28-30d), Replicate 3
|
Human embryos with Carnegie Stage 13 (E28-30d)
|
carnegie stage: S13
embryonic day: E28-30d
tissue: embryo
|
Human embryos with Carnegie Stage 13 (E28-30d), Replicate 3
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468234
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468234/suppl/GSM468234_S13_R3.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468235 | GPL96 |
|
Embryos at Carnegie Stage 14 (E31-32d), Replicate 1
|
Human embryos with Carnegie Stage 14 (E31-32d)
|
carnegie stage: S14
embryonic day: E31-32d
tissue: embryo
|
Human embryos with Carnegie Stage 14 (E31-32d), Replicate 1
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468235
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468235/suppl/GSM468235_S14_R1.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468236 | GPL96 |
|
Embryos at Carnegie Stage 14 (E31-32d), Replicate 2
|
Human embryos with Carnegie Stage 14 (E31-32d)
|
carnegie stage: S14
embryonic day: E31-32d
tissue: embryo
|
Human embryos with Carnegie Stage 14 (E31-32d), Replicate 2
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468236
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468236/suppl/GSM468236_S14_R2.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
GSM468237 | GPL96 |
|
Embryos at Carnegie Stage 14 (E31-32d), Replicate 3
|
Human embryos with Carnegie Stage 14 (E31-32d)
|
carnegie stage: S14
embryonic day: E31-32d
tissue: embryo
|
Human embryos with Carnegie Stage 14 (E31-32d), Replicate 3
The protocol for collecting human embryos was reviewed and approved by the Ethics Committee of Xinhua Hospital. The experimental procedures involving human embryos conformed to the National Ethical Guideline on Human Embryo Research issued by the Committee on Bioethics, Chinese National Human Genome Center (Southern Headquarter). Human embryos were obtained from the Department of Obstetrics and Gynecology at Xinhua Hospital during clinical drug abortion. All donors signed informed consent forms. The age of the embryo was estimated by standard clinical parameters. Great care was taken to remove contaminating extraembryonic tissues surrounding the embryo, and the embryo was frozen immediately in liquid nitrogen after washing with diethylpyrocarbonate (DEPC)-treated cold PBS. Samples were stored at -80℃ until use.
|
Sample_geo_accession | GSM468237
| Sample_status | Public on Mar 18 2010
| Sample_submission_date | Nov 04 2009
| Sample_last_update_date | Nov 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | The fragmented, biotinylated cRNA were used for hybridization at 45℃ for 17 hours with Affymetrix HG-U133A GeneChip microarrays (Affymetrix, Santa Clara, CA)
| Sample_scan_protocol | An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
| Sample_data_processing | Raw expression data were normalized using Robust Multi-array Averaging (RMA) with quantile normalization.
| Sample_platform_id | GPL96
| Sample_contact_name | Hai,,Fang
| Sample_contact_email | hfang@cs.bris.ac.uk
| Sample_contact_department | Department of Computer Science
| Sample_contact_institute | University of Bristol
| Sample_contact_address | Woodland Road
| Sample_contact_city | Bristol
| Sample_contact_zip/postal_code | BS8 1UB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468237/suppl/GSM468237_S14_R3.CEL.gz
| Sample_series_id | GSE18887
| Sample_data_row_count | 22283
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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