Search results for the GEO ID: GSE18891  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM468248 | GPL1261 |
|
mock transfected 1
|
mock transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
mock transfected
|
| Sample_geo_accession | GSM468248
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468248/suppl/GSM468248.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468248/suppl/GSM468248.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468249 | GPL1261 |
|
mock transfected 2
|
mock transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
mock transfected
|
| Sample_geo_accession | GSM468249
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468249/suppl/GSM468249.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468249/suppl/GSM468249.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468250 | GPL1261 |
|
mock transfected 3
|
mock transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
mock transfected
|
| Sample_geo_accession | GSM468250
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468250/suppl/GSM468250.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468250/suppl/GSM468250.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468251 | GPL1261 |
|
foxl2-vp16 transfected 1
|
foxl2-vp16 transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
foxl2-vp16 transfected
|
| Sample_geo_accession | GSM468251
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468251/suppl/GSM468251.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468251/suppl/GSM468251.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468252 | GPL1261 |
|
foxl2-vp16 transfected 2
|
foxl2-vp16 transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
foxl2-vp16 transfected
|
| Sample_geo_accession | GSM468252
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468252/suppl/GSM468252.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468252/suppl/GSM468252.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468253 | GPL1261 |
|
foxl2-vp16 transfected 3
|
foxl2-vp16 transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
foxl2-vp16 transfected
|
| Sample_geo_accession | GSM468253
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468253/suppl/GSM468253.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468253/suppl/GSM468253.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468254 | GPL1261 |
|
foxl2-mad transfected 1
|
foxl2-mad transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
foxl2-mad transfected
|
| Sample_geo_accession | GSM468254
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468254/suppl/GSM468254.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468254/suppl/GSM468254.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468255 | GPL1261 |
|
foxl2-mad transfected 2
|
foxl2-mad transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
foxl2-mad transfected
|
| Sample_geo_accession | GSM468255
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468255/suppl/GSM468255.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468255/suppl/GSM468255.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
| | |
|
GSM468256 | GPL1261 |
|
foxl2-mad transfected 3
|
foxl2-mad transfected
|
cell type: KK1 granulosa cells
organ: ovary
|
foxl2-mad transfected
|
| Sample_geo_accession | GSM468256
| | Sample_status | Public on Nov 05 2009
| | Sample_submission_date | Nov 04 2009
| | Sample_last_update_date | Nov 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | KK1 cells were grown in 150 mm tissue culture plates to 50% confluence. Three plates were mock transfected with 30 μl of Fugene 6 transfection reagent (Roche Applied Science; Indianapolis, IN) and 10 µg of empty vector (pcDNA 3.1). Three plates were transfected with 10 μg Foxl2-VP16 expression vector in 30 μl Fugene reagent and 3 plates were transfected with 10 μg Foxl2- MAD expression vector in 30 μl Fugene reagent.
| | Sample_growth_protocol_ch1 | KK1 cells are grown in DMEM / F12 (50/50) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento, CA), 100µg/ml penicillin-streptomycin, and 0.25 µg/ml amphotericin B. All cell culture reagents other than FBS were purchased from Mediatech; Manassas, VA.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | After 24 hours incubation at 37ºC the cells were harvested and polyA+ RNA was isolated using RNeasy (Qiagen Inc.; Valencia, CA) according to manufacturer’s procedures.
| | Sample_label_ch1 | biotinylated pseudouridine molecule
| | Sample_label_protocol_ch1 | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols. The GeneChip® Expression 3'-Amplification Reagents for IVT Labeling (IVT Labeling Kit) utilizes a single-label formulation based on a biotinylated pseudouridine molecule.
| | Sample_hyb_protocol | Performed by the Colorado State University (CSU) Affymetrix Core Facility using standard Affymetrix protocols.
| | Sample_scan_protocol | Performed by the CSU Core Facility using the Affymetrix GeneChip Scanner 3000 enabled for high resolution scanning
| | Sample_data_processing | Data were analyzed using Affymetrix® Microarray Suite (MAS) 5.0 statistical algorithms
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ken,,Escudero
| | Sample_contact_email | kfkwe00@tamuk.edu
| | Sample_contact_phone | 361-593-4069
| | Sample_contact_fax | 361-593-3800
| | Sample_contact_department | Biological & Health Sciences
| | Sample_contact_institute | Texas A&M-Kingsville
| | Sample_contact_address | 700 University Blvd MSC 158
| | Sample_contact_city | Kingsville
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 78363
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468256/suppl/GSM468256.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468256/suppl/GSM468256.CHP.gz
| | Sample_series_id | GSE18891
| | Sample_data_row_count | 45101
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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