Search results for the GEO ID: GSE18903  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM468486 | GPL570 |
|
MCF-7
|
MCF-7 breast cancer cell line
|
tissue type: breast cancer
cell line: MCF-7
|
Gene expression data from cultured MCF-7 cell line
|
| Sample_geo_accession | GSM468486
| | Sample_status | Public on Nov 06 2009
| | Sample_submission_date | Nov 05 2009
| | Sample_last_update_date | Nov 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF-7, MCF-7-14 and MDA-MB-231 cells were synchronized in S-phase with the thymidine-hydroxyurea block method.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in RPMI 1640 (Sigma) containing 10% fetal bovine serum (FBS) and antibiotic-antimycotic.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription reactions were performed using a GeneChip IVT Labeling Kit, which includes T7 RNA polymerase and Biotin-labeled ribonucleotides.
| | Sample_hyb_protocol | 15 μg of the labeled cRNA was hybridized to a Human Genome U133 Plus 2.0 Array (Affymetrix, CA, USA).
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masahiro,,Uchino
| | Sample_contact_email | uchino@biomatrix.co.jp
| | Sample_contact_institute | Bio Matrix Research, Inc
| | Sample_contact_address | 105 Higashifukai
| | Sample_contact_city | Nagareyama-shi
| | Sample_contact_state | Chiba
| | Sample_contact_zip/postal_code | 270-0101
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468486/suppl/GSM468486.CEL.gz
| | Sample_series_id | GSE18903
| | Sample_data_row_count | 54675
| | |
|
GSM468487 | GPL570 |
|
MCF-7-14
|
Invasive MCF-7-14 breast cancer cell line established from non-aggressive MCF7
|
tissue type: breast cancer
cell line: MCF-7-14
|
Gene expression data from cultured MCF-7-14 cell line
|
| Sample_geo_accession | GSM468487
| | Sample_status | Public on Nov 06 2009
| | Sample_submission_date | Nov 05 2009
| | Sample_last_update_date | Nov 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF-7, MCF-7-14 and MDA-MB-231 cells were synchronized in S-phase with the thymidine-hydroxyurea block method.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in RPMI 1640 (Sigma) containing 10% fetal bovine serum (FBS) and antibiotic-antimycotic.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription reactions were performed using a GeneChip IVT Labeling Kit, which includes T7 RNA polymerase and Biotin-labeled ribonucleotides.
| | Sample_hyb_protocol | 15 μg of the labeled cRNA was hybridized to a Human Genome U133 Plus 2.0 Array (Affymetrix, CA, USA).
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masahiro,,Uchino
| | Sample_contact_email | uchino@biomatrix.co.jp
| | Sample_contact_institute | Bio Matrix Research, Inc
| | Sample_contact_address | 105 Higashifukai
| | Sample_contact_city | Nagareyama-shi
| | Sample_contact_state | Chiba
| | Sample_contact_zip/postal_code | 270-0101
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468487/suppl/GSM468487.CEL.gz
| | Sample_series_id | GSE18903
| | Sample_data_row_count | 54675
| | |
|
GSM468488 | GPL570 |
|
MDA-MB-231
|
MDA-MB-231 breast cancer cell line
|
tissue type: breast cancer
cell line: MDA-MB-231
|
Gene expression data from cultured MDA-MB-231 cell line
|
| Sample_geo_accession | GSM468488
| | Sample_status | Public on Nov 06 2009
| | Sample_submission_date | Nov 05 2009
| | Sample_last_update_date | Nov 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF-7, MCF-7-14 and MDA-MB-231 cells were synchronized in S-phase with the thymidine-hydroxyurea block method.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in RPMI 1640 (Sigma) containing 10% fetal bovine serum (FBS) and antibiotic-antimycotic.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription reactions were performed using a GeneChip IVT Labeling Kit, which includes T7 RNA polymerase and Biotin-labeled ribonucleotides.
| | Sample_hyb_protocol | 15 μg of the labeled cRNA was hybridized to a Human Genome U133 Plus 2.0 Array (Affymetrix, CA, USA).
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masahiro,,Uchino
| | Sample_contact_email | uchino@biomatrix.co.jp
| | Sample_contact_institute | Bio Matrix Research, Inc
| | Sample_contact_address | 105 Higashifukai
| | Sample_contact_city | Nagareyama-shi
| | Sample_contact_state | Chiba
| | Sample_contact_zip/postal_code | 270-0101
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468488/suppl/GSM468488.CEL.gz
| | Sample_series_id | GSE18903
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
| Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
| Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
| |
Filter results by number of probes |
|
|
