Search results for the GEO ID: GSE18913  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM468603 | GPL570 |
|
control, VEGF 0h #1
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 0h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468603
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468603/suppl/GSM468603.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468604 | GPL570 |
|
control, VEGF 0h #2
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 0h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468604
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468604/suppl/GSM468604.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468605 | GPL570 |
|
Egr-3 siRNA #1, VEGF 0h #1
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: Egr-3 siRNA #1
stimulation: VEGF treatment for 0h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468605
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468605/suppl/GSM468605.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468606 | GPL570 |
|
Egr-3 siRNA #2, VEGF 0h #1
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: Egr-3 siRNA #2
stimulation: VEGF treatment for 0h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468606
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468606/suppl/GSM468606.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468607 | GPL570 |
|
Egr-3 siRNA #1, VEGF 0h #2
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: Egr-3 siRNA #1l
stimulation: VEGF treatment for 0h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468607
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468607/suppl/GSM468607.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468608 | GPL570 |
|
Egr-3 siRNA #2, VEGF 0h #2
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: Egr-3 siRNA #2
stimulation: VEGF treatment for 0h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468608
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468608/suppl/GSM468608.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468609 | GPL570 |
|
control, VEGF 1h #1
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 1h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468609
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468609/suppl/GSM468609.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468610 | GPL570 |
|
control, VEGF 1h #2
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 1h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468610
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468610/suppl/GSM468610.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468611 | GPL570 |
|
Egr-3 siRNA #1, VEGF 1h #1
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 1h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468611
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468611/suppl/GSM468611.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468612 | GPL570 |
|
Egr-3 siRNA #1, VEGF 1h #2
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: Egr-3 siRNA #1
stimulation: VEGF treatment for 1h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468612
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468612/suppl/GSM468612.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468613 | GPL570 |
|
Egr-3 siRNA #2, VEGF 1h #1
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: Egr-3 siRNA #2
stimulation: VEGF treatment for 1h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468613
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468613/suppl/GSM468613.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468614 | GPL570 |
|
Egr-3 siRNA #2, VEGF 1h #2
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: Egr-3 siRNA #2
stimulation: VEGF treatment for 1h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468614
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468614/suppl/GSM468614.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468615 | GPL570 |
|
control, VEGF 4h #1
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 4h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468615
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468615/suppl/GSM468615.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468616 | GPL570 |
|
control, VEGF 4h #2
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 4h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468616
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468616/suppl/GSM468616.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468617 | GPL570 |
|
Egr-3 siRNA #1, VEGF 4h #1
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: Egr-3 siRNA #1
stimulation: VEGF treatment for 4h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468617
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468617/suppl/GSM468617.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468618 | GPL570 |
|
Egr-3 siRNA #1, VEGF 4h #2
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: Egr-3 siRNA #1
stimulation: VEGF treatment for 4h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468618
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468618/suppl/GSM468618.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468619 | GPL570 |
|
Egr-3 siRNA #2, VEGF 4h #1
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: Egr-3 siRNA #2
stimulation: VEGF treatment for 4h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468619
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468619/suppl/GSM468619.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468620 | GPL570 |
|
Egr-3 siRNA #2, VEGF 4h #2
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: Egr-3 siRNA #2
stimulation: VEGF treatment for 4h
|
siRNA knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468620
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468620/suppl/GSM468620.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468621 | GPL570 |
|
control, VEGF 0h #3
|
HUVEC, VEGF treatment for 0h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 0h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468621
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468621/suppl/GSM468621.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468622 | GPL570 |
|
control, VEGF 1h #3
|
HUVEC, VEGF treatment for 1h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 1h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468622
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468622/suppl/GSM468622.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
|
GSM468623 | GPL570 |
|
control, VEGF 4h #3
|
HUVEC, VEGF treatment for 4h
|
cell: HUVEC
sirna type: control
stimulation: VEGF treatment for 4h
|
siRNA-mediated knockdown in VEGF-stimulated HUVEC
|
| Sample_geo_accession | GSM468623
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Nov 06 2009
| | Sample_last_update_date | Nov 12 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jun-ichi,,SUEHIRO
| | Sample_contact_laboratory | laboratory for systems biology and medicine
| | Sample_contact_department | RCAST
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 #34 Komaba, Meguro-ku,
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM468nnn/GSM468623/suppl/GSM468623.CEL.gz
| | Sample_series_id | GSE18913
| | Sample_data_row_count | 54675
| | |
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