Search results for the GEO ID: GSE18969 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM469548 | GPL570 |
|
obstructed fetal kidney, biological rep1
|
18 weeks obstructed
|
age: 18 weeks gestation
tissue: kidney
kidney status: obstructed
|
no additional information
|
Sample_geo_accession | GSM469548
| Sample_status | Public on Nov 11 2009
| Sample_submission_date | Nov 11 2009
| Sample_last_update_date | Nov 11 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was extracted from 5 frozen human fetal kidney samples
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed according to the manufacturer's instructions of RNesay Mini Kit (Qiagen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling of cRNA was performed according to the standard Affimemetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix. www.affymetrix.com )
| Sample_hyb_protocol | A Hybridization cocktail mixture was made for each labeled RNA sample. The cocktail icluded spikes of GeneChip® hybridization controls. Each sample was hybridized to an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Sixteen hour hybridizations were performed in a GeneChip® Hybridization Oven 640. This was followed by automated washes and staining in a GeneChip® Fluidics Station 450 contolled by GeneChip® Operating Software (GCOS).
| Sample_scan_protocol | The scanning and raw image capture was performed with a solidstate green laser GeneChip® Scanner 3000 7G.
| Sample_data_processing | Statistical analysis for the gene microarray data was conducted using the LMGene software for data transformation and identification of differentially expressed genes in gene expression arrays, and data was normalized using Affy/RMA. The new version 2.6.0 of R was used for the analysis. To identify differentially expressed genes, posterior-FDR p value was computed for each probe set.
| Sample_platform_id | GPL570
| Sample_contact_name | Douglas ,,Matsell
| Sample_contact_email | dmatsell@cw.bc.ca
| Sample_contact_phone | 604 875-2272
| Sample_contact_fax | 604 875-3649
| Sample_contact_institute | BC Children's Hospital
| Sample_contact_address | 4480 Oak Street, Rm K4-150
| Sample_contact_city | Vancouver
| Sample_contact_state | BC
| Sample_contact_zip/postal_code | V6H 3V4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469548/suppl/GSM469548.CEL.gz
| Sample_series_id | GSE18969
| Sample_data_row_count | 54675
| |
|
GSM469549 | GPL570 |
|
obstructed fetal kidney, biological rep2
|
18 weeks obstructed
|
age: 18 weeks gestation
tissue: kidney
kidney status: obstructed
|
no additional information
|
Sample_geo_accession | GSM469549
| Sample_status | Public on Nov 11 2009
| Sample_submission_date | Nov 11 2009
| Sample_last_update_date | Nov 11 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was extracted from 5 frozen human fetal kidney samples
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed according to the manufacturer's instructions of RNesay Mini Kit (Qiagen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling of cRNA was performed according to the standard Affimemetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix. www.affymetrix.com )
| Sample_hyb_protocol | A Hybridization cocktail mixture was made for each labeled RNA sample. The cocktail icluded spikes of GeneChip® hybridization controls. Each sample was hybridized to an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Sixteen hour hybridizations were performed in a GeneChip® Hybridization Oven 640. This was followed by automated washes and staining in a GeneChip® Fluidics Station 450 contolled by GeneChip® Operating Software (GCOS).
| Sample_scan_protocol | The scanning and raw image capture was performed with a solidstate green laser GeneChip® Scanner 3000 7G.
| Sample_data_processing | Statistical analysis for the gene microarray data was conducted using the LMGene software for data transformation and identification of differentially expressed genes in gene expression arrays, and data was normalized using Affy/RMA. The new version 2.6.0 of R was used for the analysis. To identify differentially expressed genes, posterior-FDR p value was computed for each probe set.
| Sample_platform_id | GPL570
| Sample_contact_name | Douglas ,,Matsell
| Sample_contact_email | dmatsell@cw.bc.ca
| Sample_contact_phone | 604 875-2272
| Sample_contact_fax | 604 875-3649
| Sample_contact_institute | BC Children's Hospital
| Sample_contact_address | 4480 Oak Street, Rm K4-150
| Sample_contact_city | Vancouver
| Sample_contact_state | BC
| Sample_contact_zip/postal_code | V6H 3V4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469549/suppl/GSM469549.CEL.gz
| Sample_series_id | GSE18969
| Sample_data_row_count | 54675
| |
|
GSM469550 | GPL570 |
|
normal fetal kidney, biological rep1
|
18 weeks normal
|
age: 18 weeks gestation
tissue: kidney
kidney status: normal
|
no additional information
|
Sample_geo_accession | GSM469550
| Sample_status | Public on Nov 11 2009
| Sample_submission_date | Nov 11 2009
| Sample_last_update_date | Nov 11 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was extracted from 5 frozen human fetal kidney samples
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed according to the manufacturer's instructions of RNesay Mini Kit (Qiagen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling of cRNA was performed according to the standard Affimemetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix. www.affymetrix.com )
| Sample_hyb_protocol | A Hybridization cocktail mixture was made for each labeled RNA sample. The cocktail icluded spikes of GeneChip® hybridization controls. Each sample was hybridized to an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Sixteen hour hybridizations were performed in a GeneChip® Hybridization Oven 640. This was followed by automated washes and staining in a GeneChip® Fluidics Station 450 contolled by GeneChip® Operating Software (GCOS).
| Sample_scan_protocol | The scanning and raw image capture was performed with a solidstate green laser GeneChip® Scanner 3000 7G.
| Sample_data_processing | Statistical analysis for the gene microarray data was conducted using the LMGene software for data transformation and identification of differentially expressed genes in gene expression arrays, and data was normalized using Affy/RMA. The new version 2.6.0 of R was used for the analysis. To identify differentially expressed genes, posterior-FDR p value was computed for each probe set.
| Sample_platform_id | GPL570
| Sample_contact_name | Douglas ,,Matsell
| Sample_contact_email | dmatsell@cw.bc.ca
| Sample_contact_phone | 604 875-2272
| Sample_contact_fax | 604 875-3649
| Sample_contact_institute | BC Children's Hospital
| Sample_contact_address | 4480 Oak Street, Rm K4-150
| Sample_contact_city | Vancouver
| Sample_contact_state | BC
| Sample_contact_zip/postal_code | V6H 3V4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469550/suppl/GSM469550.CEL.gz
| Sample_series_id | GSE18969
| Sample_data_row_count | 54675
| |
|
GSM469551 | GPL570 |
|
normal fetal kidney, biological rep2
|
8 weeks normal
|
age: 8 weeks gestation
tissue: kidney
kidney status: normal
|
no additional information
|
Sample_geo_accession | GSM469551
| Sample_status | Public on Nov 11 2009
| Sample_submission_date | Nov 11 2009
| Sample_last_update_date | Nov 11 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was extracted from 5 frozen human fetal kidney samples
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed according to the manufacturer's instructions of RNesay Mini Kit (Qiagen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling of cRNA was performed according to the standard Affimemetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix. www.affymetrix.com )
| Sample_hyb_protocol | A Hybridization cocktail mixture was made for each labeled RNA sample. The cocktail icluded spikes of GeneChip® hybridization controls. Each sample was hybridized to an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Sixteen hour hybridizations were performed in a GeneChip® Hybridization Oven 640. This was followed by automated washes and staining in a GeneChip® Fluidics Station 450 contolled by GeneChip® Operating Software (GCOS).
| Sample_scan_protocol | The scanning and raw image capture was performed with a solidstate green laser GeneChip® Scanner 3000 7G.
| Sample_data_processing | Statistical analysis for the gene microarray data was conducted using the LMGene software for data transformation and identification of differentially expressed genes in gene expression arrays, and data was normalized using Affy/RMA. The new version 2.6.0 of R was used for the analysis. To identify differentially expressed genes, posterior-FDR p value was computed for each probe set.
| Sample_platform_id | GPL570
| Sample_contact_name | Douglas ,,Matsell
| Sample_contact_email | dmatsell@cw.bc.ca
| Sample_contact_phone | 604 875-2272
| Sample_contact_fax | 604 875-3649
| Sample_contact_institute | BC Children's Hospital
| Sample_contact_address | 4480 Oak Street, Rm K4-150
| Sample_contact_city | Vancouver
| Sample_contact_state | BC
| Sample_contact_zip/postal_code | V6H 3V4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469551/suppl/GSM469551.CEL.gz
| Sample_series_id | GSE18969
| Sample_data_row_count | 54675
| |
|
GSM469552 | GPL570 |
|
normal fetal kidney, biological rep3
|
8 weeks normal
|
age: 8 weeks gestation
tissue: kidney
kidney status: normal
|
no additional information
|
Sample_geo_accession | GSM469552
| Sample_status | Public on Nov 11 2009
| Sample_submission_date | Nov 11 2009
| Sample_last_update_date | Nov 11 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was extracted from 5 frozen human fetal kidney samples
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed according to the manufacturer's instructions of RNesay Mini Kit (Qiagen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling of cRNA was performed according to the standard Affimemetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix. www.affymetrix.com )
| Sample_hyb_protocol | A Hybridization cocktail mixture was made for each labeled RNA sample. The cocktail icluded spikes of GeneChip® hybridization controls. Each sample was hybridized to an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Sixteen hour hybridizations were performed in a GeneChip® Hybridization Oven 640. This was followed by automated washes and staining in a GeneChip® Fluidics Station 450 contolled by GeneChip® Operating Software (GCOS).
| Sample_scan_protocol | The scanning and raw image capture was performed with a solidstate green laser GeneChip® Scanner 3000 7G.
| Sample_data_processing | Statistical analysis for the gene microarray data was conducted using the LMGene software for data transformation and identification of differentially expressed genes in gene expression arrays, and data was normalized using Affy/RMA. The new version 2.6.0 of R was used for the analysis. To identify differentially expressed genes, posterior-FDR p value was computed for each probe set.
| Sample_platform_id | GPL570
| Sample_contact_name | Douglas ,,Matsell
| Sample_contact_email | dmatsell@cw.bc.ca
| Sample_contact_phone | 604 875-2272
| Sample_contact_fax | 604 875-3649
| Sample_contact_institute | BC Children's Hospital
| Sample_contact_address | 4480 Oak Street, Rm K4-150
| Sample_contact_city | Vancouver
| Sample_contact_state | BC
| Sample_contact_zip/postal_code | V6H 3V4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469552/suppl/GSM469552.CEL.gz
| Sample_series_id | GSE18969
| Sample_data_row_count | 54675
| |
|
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