Result

Search results for the GEO ID: GSE18991

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Title

Source name

Description

Characteristics

GSM469866

GPL1261

chondrocytes at E18.5, control, biological rep1

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: wild type

Mouse_270TA

Sample_geo_accession	GSM469866
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469866/suppl/GSM469866.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469866/suppl/GSM469866.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469867

GPL1261

chondrocytes at E18.5, control, biological rep2

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: wild type

Mouse_271TA

Sample_geo_accession	GSM469867
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469867/suppl/GSM469867.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469867/suppl/GSM469867.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469868

GPL1261

chondrocytes at E18.5, control, biological rep3

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: wild type

Mouse_273TA

Sample_geo_accession	GSM469868
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469868/suppl/GSM469868.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469868/suppl/GSM469868.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469869

GPL1261

chondrocytes at E18.5, control, biological rep4

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: wild type

Mouse_291TA

Sample_geo_accession	GSM469869
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469869/suppl/GSM469869.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469869/suppl/GSM469869.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469870

GPL1261

chondrocytes at E18.5, transgenic, biological rep1

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: Hoxd4 transgenic

Mouse_270TR

Sample_geo_accession	GSM469870
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469870/suppl/GSM469870.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469870/suppl/GSM469870.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469871

GPL1261

chondrocytes at E18.5, transgenic, biological rep2

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: Hoxd4 transgenic

Mouse_271TR

Sample_geo_accession	GSM469871
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469871/suppl/GSM469871.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469871/suppl/GSM469871.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469872

GPL1261

chondrocytes at E18.5, transgenic, biological rep3

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: Hoxd4 transgenic

Mouse_273TR

Sample_geo_accession	GSM469872
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469872/suppl/GSM469872.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469872/suppl/GSM469872.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

GSM469873

GPL1261

chondrocytes at E18.5, transgenic, biological rep4

Mouse rib chondrocytes

strain: FVB age: E18.5 tissue: primary chondrocytes genome/variation: Hoxd4 transgenic

Mouse_291TR

Sample_geo_accession	GSM469873
Sample_status	Public on Oct 15 2010
Sample_submission_date	Nov 12 2009
Sample_last_update_date	Nov 12 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Rib cages were dissected from E18.5 embryos and washed in PBS. Cells were dissociated by enzymatic digestion with 0.25% Collagenase and 0.25% Trypsin/EDTA for 1h at 37oC. The rib cages were washed, and fresh 0.25% Collagenase in PBS was added for 1h at 37oC. Enzymatic reaction was stopped by adding an equal volume of tissue culture medium containing 10% FCS. The cells were filtered through a 70 micrometer cell strainer and pelleted (1200rpm for 10min). Pellets were washed with PBS and stored in Trizol reagent.
Sample_growth_protocol_ch1	Pregnant dams were single housed, the morning with presence of a vaginal plug being counted as 0.5 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469873/suppl/GSM469873.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM469nnn/GSM469873/suppl/GSM469873.CHP.gz
Sample_series_id	GSE18991
Sample_series_id	GSE19002
Sample_data_row_count	45101

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