Result

Search results for the GEO ID: GSE19060

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM471665

GPL570

Cells isolated from Normal Meniscal tissue 1

Meniscal Tissue

tissue: Normal

Passage 2 cells

Sample_geo_accession	GSM471665
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471665/suppl/GSM471665.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471666

GPL570

Cells isolated from Normal Meniscal tissue 2

Meniscal Tissue

tissue: Normal

Passage 2 cells

Sample_geo_accession	GSM471666
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471666/suppl/GSM471666.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471667

GPL570

Cells isolated from Normal Meniscal tissue 3

Meniscal Tissue

tissue: Normal

Passage 2 cells

Sample_geo_accession	GSM471667
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471667/suppl/GSM471667.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471668

GPL570

Cells isolated from OA Meniscal tissue 1

Meniscal Tissue

tissue: OA

Passage 2 cells

Sample_geo_accession	GSM471668
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471668/suppl/GSM471668.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471669

GPL570

Cells isolated from OA Meniscal tissue 2

Meniscal Tissue

tissue: OA

Passage 2 cells

Sample_geo_accession	GSM471669
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471669/suppl/GSM471669.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471670

GPL570

Cells isolated from OA Meniscal tissue 3

Meniscal Tissue

tissue: OA

Passage 2 cells

Sample_geo_accession	GSM471670
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471670/suppl/GSM471670.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471671

GPL570

Cells isolated from OA Meniscal tissue 4

Meniscal Tissue

tissue: OA

Passage 2 cells

Sample_geo_accession	GSM471671
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471671/suppl/GSM471671.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

GSM471672

GPL570

Cells isolated from OA Meniscal tissue 5

Meniscal Tissue

tissue: OA

Passage 2 cells

Sample_geo_accession	GSM471672
Sample_status	Public on Nov 18 2009
Sample_submission_date	Nov 17 2009
Sample_last_update_date	Nov 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Cells were seeded in 100 mm plates at 75% confluence and incubated at 37oC in Dulbecco’s modified eagle medium containing 0.5% antibiotic/antimycotic solution and 10% fetal bovin serum for 24 hours. Cells were incubated an additional 48 hours with the medium (containing only 1% serum) being changed every 24 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized and biotin labeled using BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY)
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL570
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM471nnn/GSM471672/suppl/GSM471672.CEL.gz
Sample_series_id	GSE19060
Sample_data_row_count	54675

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