Search results for the GEO ID: GSE19098  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM473026 | GPL570 |
|
HUVEC_spread_noVEGF_rep1
|
HUVECs fully spread, no VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473026
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473026/suppl/GSM473026.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473027 | GPL570 |
|
HUVEC_spread_noVEGF_rep2
|
HUVECs fully spread, no VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473027
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473027/suppl/GSM473027.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473028 | GPL570 |
|
HUVEC_spread_noVEGF_rep3
|
HUVECs fully spread, no VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473028
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473028/suppl/GSM473028.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473029 | GPL570 |
|
HUVEC_spread_VEGF_rep1
|
HUVECs fully spread, with VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473029
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473029/suppl/GSM473029.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473030 | GPL570 |
|
HUVEC_spread_VEGF_rep2
|
HUVECs fully spread, with VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473030
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473030/suppl/GSM473030.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473031 | GPL570 |
|
HUVEC_spread_VEGF_rep3
|
HUVECs fully spread, with VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473031
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473031/suppl/GSM473031.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473032 | GPL570 |
|
HUVEC_unspread_noVEGF_rep1
|
HUVECs restricted in spreading, no VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473032
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473032/suppl/GSM473032.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473033 | GPL570 |
|
HUVEC_unspread_noVEGF_rep2
|
HUVECs restricted in spreading, no VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473033
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473033/suppl/GSM473033.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473034 | GPL570 |
|
HUVEC_unspread_noVEGF_rep3
|
HUVECs restricted in spreading, no VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473034
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473034/suppl/GSM473034.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473035 | GPL570 |
|
HUVEC_unspread_VEGF_rep1
|
HUVECs restricted in spreading, with VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473035
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473035/suppl/GSM473035.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
|
GSM473036 | GPL570 |
|
HUVEC_unspread_VEGF_rep2
|
HUVECs restricted in spreading, with VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473036
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473036/suppl/GSM473036.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
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GSM473037 | GPL570 |
|
HUVEC_unspread_VEGF_rep3
|
HUVECs restricted in spreading, with VEGF exposure
|
cell type: primary HUVECs
cell passage: passage 3
|
Gene expression data from HUVECs with changes in ECM adhesion and soluble VEGF stimulation
|
| Sample_geo_accession | GSM473037
| | Sample_status | Public on Dec 15 2010
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Dec 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVECs were cultured on micropatterned islands of fibronectin or allowed to spread fully on fibronectin for 2 hrs and then stimulated with 50ng/ml VEGF or no growth factor for 18 hrs in starve media (5% FBS in M199).
| | Sample_growth_protocol_ch1 | HUVECs were cultured in full growth medium (M199+20%FBS+ 50mg/ml ECGS+100mg/ml heparin+100units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces prior to treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extrated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Affymetrix U133+ v2.0 arrays. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
| | Sample_data_processing | The data were analyzed using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Colette,,Shen
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 210 S. 33rd St., 510 Skirkanich Hall
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473037/suppl/GSM473037.CEL.gz
| | Sample_series_id | GSE19098
| | Sample_data_row_count | 54675
| | |
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