Search results for the GEO ID: GSE19106  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM473603 | GPL1355 |
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RASMC_VEH_rep1
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Rat aortic smooth muscle cells, VEH-treated, 3 hrs
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tissue: vascular smooth muscle
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RASMC_VEH_rep1
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| Sample_geo_accession | GSM473603
| | Sample_status | Public on Nov 20 2009
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Nov 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASMCs were first washed with PBS and stimulated with either PDGF-BB (30ng/mL) or vehicle (0.2% BSA in 10mM acetic acid) in serum-free, insulin-free media. RASMCs were treated for 3 hours.
| | Sample_growth_protocol_ch1 | RASMCs were plated at 1E4 cells/cm2 in 10% FBS for 24 hrs. RASMCs were then serum-starved for 72 hrs in serum-free, insulin-free media.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit spin columns were used for RNA extraction (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | cRNA was hybridized accoring to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Raw .CEL files were normalized using invariant set normalization and the PM-MM model of expression was used to contruct gene expression measures and standard errors for each probe set (dCHIP).
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Brian,R,Wamhoff
| | Sample_contact_email | wamhoff@virginia.edu
| | Sample_contact_phone | 434-243-6525
| | Sample_contact_fax | 434-982-3139
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 409 Lane Road Bldg MR4 Rm6022
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473603/suppl/GSM473603.CEL.gz
| | Sample_series_id | GSE19106
| | Sample_data_row_count | 23764
| | |
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GSM473604 | GPL1355 |
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RASMC_VEH_rep2
|
Rat aortic smooth muscle cells, VEH-treated, 3 hrs
|
tissue: vascular smooth muscle
|
RASMC_VEH_rep2
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| Sample_geo_accession | GSM473604
| | Sample_status | Public on Nov 20 2009
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Nov 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASMCs were first washed with PBS and stimulated with either PDGF-BB (30ng/mL) or vehicle (0.2% BSA in 10mM acetic acid) in serum-free, insulin-free media. RASMCs were treated for 3 hours.
| | Sample_growth_protocol_ch1 | RASMCs were plated at 1E4 cells/cm2 in 10% FBS for 24 hrs. RASMCs were then serum-starved for 72 hrs in serum-free, insulin-free media.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit spin columns were used for RNA extraction (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | cRNA was hybridized accoring to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Raw .CEL files were normalized using invariant set normalization and the PM-MM model of expression was used to contruct gene expression measures and standard errors for each probe set (dCHIP).
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Brian,R,Wamhoff
| | Sample_contact_email | wamhoff@virginia.edu
| | Sample_contact_phone | 434-243-6525
| | Sample_contact_fax | 434-982-3139
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 409 Lane Road Bldg MR4 Rm6022
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473604/suppl/GSM473604.CEL.gz
| | Sample_series_id | GSE19106
| | Sample_data_row_count | 23764
| | |
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GSM473605 | GPL1355 |
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RASMC_BB_rep1
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Rat aortic smooth muscle cells, PDGF-BB-treated, 3 hrs
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tissue: vascular smooth muscle
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RASMC_BB_rep1
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| Sample_geo_accession | GSM473605
| | Sample_status | Public on Nov 20 2009
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Nov 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASMCs were first washed with PBS and stimulated with either PDGF-BB (30ng/mL) or vehicle (0.2% BSA in 10mM acetic acid) in serum-free, insulin-free media. RASMCs were treated for 3 hours.
| | Sample_growth_protocol_ch1 | RASMCs were plated at 1E4 cells/cm2 in 10% FBS for 24 hrs. RASMCs were then serum-starved for 72 hrs in serum-free, insulin-free media.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit spin columns were used for RNA extraction (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | cRNA was hybridized accoring to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Raw .CEL files were normalized using invariant set normalization and the PM-MM model of expression was used to contruct gene expression measures and standard errors for each probe set (dCHIP).
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Brian,R,Wamhoff
| | Sample_contact_email | wamhoff@virginia.edu
| | Sample_contact_phone | 434-243-6525
| | Sample_contact_fax | 434-982-3139
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 409 Lane Road Bldg MR4 Rm6022
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473605/suppl/GSM473605.CEL.gz
| | Sample_series_id | GSE19106
| | Sample_data_row_count | 23764
| | |
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GSM473606 | GPL1355 |
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RASMC_BB_rep2
|
Rat aortic smooth muscle cells, PDGF-BB-treated, 3 hrs
|
tissue: vascular smooth muscle
|
RASMC_BB_rep2
|
| Sample_geo_accession | GSM473606
| | Sample_status | Public on Nov 20 2009
| | Sample_submission_date | Nov 19 2009
| | Sample_last_update_date | Nov 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASMCs were first washed with PBS and stimulated with either PDGF-BB (30ng/mL) or vehicle (0.2% BSA in 10mM acetic acid) in serum-free, insulin-free media. RASMCs were treated for 3 hours.
| | Sample_growth_protocol_ch1 | RASMCs were plated at 1E4 cells/cm2 in 10% FBS for 24 hrs. RASMCs were then serum-starved for 72 hrs in serum-free, insulin-free media.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit spin columns were used for RNA extraction (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | cRNA was hybridized accoring to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Raw .CEL files were normalized using invariant set normalization and the PM-MM model of expression was used to contruct gene expression measures and standard errors for each probe set (dCHIP).
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Brian,R,Wamhoff
| | Sample_contact_email | wamhoff@virginia.edu
| | Sample_contact_phone | 434-243-6525
| | Sample_contact_fax | 434-982-3139
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 409 Lane Road Bldg MR4 Rm6022
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM473nnn/GSM473606/suppl/GSM473606.CEL.gz
| | Sample_series_id | GSE19106
| | Sample_data_row_count | 23764
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