Search results for the GEO ID: GSE19128  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM474463 | GPL570 |
|
Untreated PBMCs after 18 hours
|
Untreated PBMCs after 18 hours
|
cell type: PBMC
agent: mock
time point: 18hrs
|
Untreated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474463
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474463/suppl/GSM474463.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474463/suppl/GSM474463.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474464 | GPL570 |
|
Ethanol treated PBMCs after 18 hours
|
Ethanol treated PBMCs after 18 hours
|
cell type: PBMC
agent: Ethanol
time point: 18hrs
|
Ethanol treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474464
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474464/suppl/GSM474464.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474464/suppl/GSM474464.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474465 | GPL570 |
|
Astragalus treated PBMCs after 3 hours
|
Astragalus treated PBMCs after 3 hours
|
cell type: PBMC
agent: Astragalus
time point: 3hrs
|
Astragalus treated PBMCs after 3 hours
|
| Sample_geo_accession | GSM474465
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474465/suppl/GSM474465.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474465/suppl/GSM474465.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474466 | GPL570 |
|
Astragalus treated PBMCs after 8 hours
|
Astragalus treated PBMCs after 8 hours
|
cell type: PBMC
agent: Astragalus
time point: 8hrs
|
Astragalus treated PBMCs after 8 hours
|
| Sample_geo_accession | GSM474466
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474466/suppl/GSM474466.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474466/suppl/GSM474466.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474467 | GPL570 |
|
Astragalus treated PBMCs after 18 hours
|
Astragalus treated PBMCs after 18 hours
|
cell type: PBMC
agent: Astragalus
time point: 18hrs
|
Astragalus treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474467
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474467/suppl/GSM474467.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474467/suppl/GSM474467.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474468 | GPL570 |
|
Ethanol treated PBMCs after 18 hours, 2
|
Ethanol treated PBMCs after 18 hours
|
cell type: PBMC
agent: Ethanol
time point: 18hrs
|
Ethanol treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474468
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474468/suppl/GSM474468.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474468/suppl/GSM474468.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474469 | GPL570 |
|
Sambucus treated PBMCs after 18 hours
|
Sambucus treated PBMCs after 18 hours
|
cell type: PBMC
agent: Sambucus
time point: 18hrs
|
Sambucus treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474469
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474469/suppl/GSM474469.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474469/suppl/GSM474469.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474470 | GPL570 |
|
Andrographis treated PBMCs after 18 hours
|
Andrographis treated PBMCs after 18 hours
|
cell type: PBMC
agent: Andrographis
time point: 18hrs
|
Andrographis treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474470
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474470/suppl/GSM474470.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474470/suppl/GSM474470.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474471 | GPL570 |
|
Astragalus treated PBMCs after 18 hours, 2
|
Astragalus treated PBMCs after 18 hours
|
cell type: PBMC
agent: Astragalus
time point: 18hrs
|
Astragalus treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474471
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474471/suppl/GSM474471.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474471/suppl/GSM474471.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474472 | GPL570 |
|
Urtica treated PBMCs after 18 hours
|
Urtica treated PBMCs after 18 hours
|
cell type: PBMC
agent: Urtica
time point: 18hrs
|
Urtica treated PBMCs after 18 hours
|
| Sample_geo_accession | GSM474472
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474472/suppl/GSM474472.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474472/suppl/GSM474472.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
| | |
|
GSM474473 | GPL570 |
|
Ethanol treated PBMCs after 18 hours, 3
|
Ethanol treated PBMCs after 18 hours
|
cell type: PBMC
agent: Ethanol
time point: 18hrs
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Ethanol treated PBMCs after 18 hours
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| Sample_geo_accession | GSM474473
| | Sample_status | Public on Nov 21 2009
| | Sample_submission_date | Nov 20 2009
| | Sample_last_update_date | Nov 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 x 10^7 cells were treated with 5 ul herbal extract for the indicated times. Treatment of cells with phorbol-12-myristate-13-acetate (Calbiochem) was done at a final concentration of 50 nm.
| | Sample_growth_protocol_ch1 | PBMCs were isolated from whole blood using Ficoll-Paque Plus system (Amersham Biosciences) and then resuspended in RPMI1640 with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated and purified as per manufacturers protocol using an Rneasy Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was made using the Affymetrix One-Cycle Kit
| | Sample_hyb_protocol | Total of 200 uL of each hybridization cocktail was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array for 16 hours at 45 deg. C in the hybridization Oven 640.
| | Sample_scan_protocol | Affymetrix Genechip Scanner 3000 7G
| | Sample_data_processing | Data were analyzed using Affymetrix Genechip Operating System Version 1.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Waters
| | Sample_contact_email | robert.f.waters@asu.edu
| | Sample_contact_laboratory | Center for Infectious Disease and Vaccinology
| | Sample_contact_department | Biodesign Institute
| | Sample_contact_institute | Arizona State University
| | Sample_contact_address | 1001 S. McAllister
| | Sample_contact_city | Tempe
| | Sample_contact_state | AZ
| | Sample_contact_zip/postal_code | 85287
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474473/suppl/GSM474473.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474473/suppl/GSM474473.CHP.gz
| | Sample_series_id | GSE19128
| | Sample_data_row_count | 54675
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