Search results for the GEO ID: GSE19136  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM474493 | GPL570 |
|
human_LIMA_artery_patient1_control
|
Human LIMA artery from patient 1, control (unstented)
|
gender: male
individual: patient 1
treatment: control
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474493
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474493/suppl/GSM474493.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474494 | GPL570 |
|
human_LIMA_artery_patient1_bare_metal_cardiovascular_stent
|
Human LIMA artery from patient 1, treatment: bare metal cardiovascular stent
|
gender: male
individual: patient 1
treatment: bare metal cardiovascular stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474494
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474494/suppl/GSM474494.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474495 | GPL570 |
|
human_LIMA_artery_patient1_paclitaxel-eluting_stent
|
Human LIMA artery from patient 1, treatment: paclitaxel-eluting stent
|
gender: male
individual: patient 1
treatment: paclitaxel-eluting stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474495
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474495/suppl/GSM474495.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474496 | GPL570 |
|
human_LIMA_artery_patient2_control
|
Human LIMA artery from patient 2, control (unstented)
|
gender: male
individual: patient 2
treatment: control
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474496
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474496/suppl/GSM474496.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474497 | GPL570 |
|
human_LIMA_artery_patient2_bare_metal_cardiovascular_stent
|
Human LIMA artery from patient 2, treatment: bare metal cardiovascular stent
|
gender: male
individual: patient 2
treatment: bare metal cardiovascular stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474497
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474497/suppl/GSM474497.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474498 | GPL570 |
|
human_LIMA_artery_patient2_paclitaxel-eluting_stent
|
Human LIMA artery from patient 2, treatment: paclitaxel-eluting stent
|
gender: male
individual: patient 2
treatment: paclitaxel-eluting stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474498
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474498/suppl/GSM474498.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474499 | GPL570 |
|
human_LIMA_artery_patient4_control
|
Human LIMA artery from patient 4, control (unstented)
|
gender: male
individual: patient 4
treatment: control
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474499
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474499/suppl/GSM474499.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474500 | GPL570 |
|
human_LIMA_artery_patient4_bare_metal_cardiovascular_stent
|
Human LIMA artery from patient 4, treatment: bare metal cardiovascular stent
|
gender: male
individual: patient 4
treatment: bare metal cardiovascular stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474500
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474500/suppl/GSM474500.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474501 | GPL570 |
|
human_LIMA_artery_patient4_paclitaxel-eluting_stent
|
Human LIMA artery from patient 4, treatment: paclitaxel-eluting stent
|
gender: male
individual: patient 4
treatment: paclitaxel-eluting stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474501
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474501/suppl/GSM474501.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474502 | GPL570 |
|
human_LIMA_artery_patient5_control
|
Human LIMA artery from patient 5, control (unstented)
|
gender: male
individual: patient 5
treatment: control
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474502
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474502/suppl/GSM474502.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474503 | GPL570 |
|
human_LIMA_artery_patient5_bare_metal_cardiovascular_stent
|
Human LIMA artery from patient 5, treatment: bare metal cardiovascular stent
|
gender: male
individual: patient 5
treatment: bare metal cardiovascular stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474503
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474503/suppl/GSM474503.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
GSM474504 | GPL570 |
|
human_LIMA_artery_patient5_paclitaxel-eluting_stent
|
Human LIMA artery from patient 5, treatment: paclitaxel-eluting stent
|
gender: male
individual: patient 5
treatment: paclitaxel-eluting stent
|
stable angina and 3-vessel coronary atheroslerosis
|
| Sample_geo_accession | GSM474504
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Nov 23 2009
| | Sample_last_update_date | Nov 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ann-Sofie,,Albrekt
| | Sample_contact_email | ann-sofie.albrekt@immun.lth.se
| | Sample_contact_phone | +46462229818
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Solvegatan 19
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 22184
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM474nnn/GSM474504/suppl/GSM474504.CEL.gz
| | Sample_series_id | GSE19136
| | Sample_data_row_count | 54675
| | |
|
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