Search results for the GEO ID: GSE19317  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM479998 | GPL570 |
|
Filtered air exposure 1 -baseline
|
Circulating mononuclear cells before exposure
|
cell type: mononuclear cells
agent: FA
time: 0 hour
|
Gene expression data from circulating mononuclear cells before exposure
|
| Sample_geo_accession | GSM479998
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM479nnn/GSM479998/suppl/GSM479998.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM479999 | GPL570 |
|
Filtered air exposure 1 -24 hours post-exposure
|
Circulating mononuclear cells after exposure
|
cell type: mononuclear cells
agent: FA
time: 24 hours
|
Gene expression data from circulating mononuclear cells after filtered air exposure
|
| Sample_geo_accession | GSM479999
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM479nnn/GSM479999/suppl/GSM479999.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480000 | GPL570 |
|
Ultrafine particle exposure 1 -baseline
|
Circulating mononuclear cells before exposure
|
cell type: mononuclear cells
agent: UFP
time: 0 hour
|
Gene expression data from circulating mononuclear cells before exposure
|
| Sample_geo_accession | GSM480000
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480000/suppl/GSM480000.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480001 | GPL570 |
|
Ultrafine particle exposure 1 -24 hours post-exposure
|
Circulating mononuclear cells after exposure
|
cell type: mononuclear cells
agent: UFP
time: 24 hours
|
Gene expression data from circulating mononuclear cells after ultrafine carbon particle exposure
|
| Sample_geo_accession | GSM480001
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480001/suppl/GSM480001.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480002 | GPL570 |
|
Filtered air exposure 2 -baseline
|
Circulating mononuclear cells before exposure
|
cell type: mononuclear cells
agent: FA
time: 0 hour
|
Gene expression data from circulating mononuclear cells before exposure
|
| Sample_geo_accession | GSM480002
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480002/suppl/GSM480002.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480003 | GPL570 |
|
Filtered air exposure 2 -24 hours post-exposure
|
Circulating mononuclear cells after exposure
|
cell type: mononuclear cells
agent: FA
time: 24 hours
|
Gene expression data from circulating mononuclear cells after filtered air exposure
|
| Sample_geo_accession | GSM480003
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480003/suppl/GSM480003.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480004 | GPL570 |
|
Ultrafine particle exposure 2 -baseline
|
Circulating mononuclear cells before exposure
|
cell type: mononuclear cells
agent: UFP
time: 0 hour
|
Gene expression data from circulating mononuclear cells before exposure
|
| Sample_geo_accession | GSM480004
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480004/suppl/GSM480004.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480005 | GPL570 |
|
Ultrafine particle exposure 2 -24 hours post-exposure
|
Circulating mononuclear cells after exposure
|
cell type: mononuclear cells
agent: UFP
time: 24 hours
|
Gene expression data from circulating mononuclear cells after ultrafine carbon particle exposure
|
| Sample_geo_accession | GSM480005
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480005/suppl/GSM480005.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480006 | GPL570 |
|
Filtered air exposure 3 -baseline
|
Circulating mononuclear cells before exposure
|
cell type: mononuclear cells
agent: FA
time: 0 hour
|
Gene expression data from circulating mononuclear cells before exposure
|
| Sample_geo_accession | GSM480006
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480006/suppl/GSM480006.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480007 | GPL570 |
|
Filtered air exposure 3 -24 hours post-exposure
|
Circulating mononuclear cells after exposure
|
cell type: mononuclear cells
agent: FA
time: 24 hours
|
Gene expression data from circulating mononuclear cells after filtered air exposure
|
| Sample_geo_accession | GSM480007
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480007/suppl/GSM480007.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
| | |
|
GSM480008 | GPL570 |
|
Ultrafine particle exposure 3 -baseline
|
Circulating mononuclear cells before exposure
|
cell type: mononuclear cells
agent: UFP
time: 0 hour
|
Gene expression data from circulating mononuclear cells before exposure
|
| Sample_geo_accession | GSM480008
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480008/suppl/GSM480008.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
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GSM480009 | GPL570 |
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Ultrafine particle exposure 3 -24 hours post-exposure
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Circulating mononuclear cells after exposure
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cell type: mononuclear cells
agent: UFP
time: 24 hours
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Gene expression data from circulating mononuclear cells after ultrafine carbon particle exposure
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| Sample_geo_accession | GSM480009
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Dec 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Subjects underwent a 2-hr mouthpiece exposure to either filtered air (FA) or UFP (50 ug/m3), with four 15-min exercise periods on a bicycle ergometer (target minute ventilation 20 L/min/m2 body surface area). Peripheral blood samples were obtained before exposure (0 hour) and at 24 hours after exposure
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using TRIZOL®LS (Invitrogen Corp., Carlsbad, CA), amplified by the Affymetrix Two-Cycle cDNA synthesis kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000
| | Sample_data_processing | The data were RMA-normalized and analyzed using Partek Genomics Suite 6.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuh-Chin,Tony,Huang
| | Sample_contact_email | huang002@mc.duke.edu
| | Sample_contact_phone | 919-684-3069
| | Sample_contact_laboratory | Pulmonary and Critical Care
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | Erwin Rd
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480009/suppl/GSM480009.CEL.gz
| | Sample_series_id | GSE19317
| | Sample_data_row_count | 54675
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