Search results for the GEO ID: GSE19330  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM480283 | GPL570 |
|
Subject 1188, Replicate 1
|
Derived from neonatal foreskin
|
subject/donor: 1188
gender: Male
tissue: cultured epidermis
replicate: 1
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480283
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480283/suppl/GSM480283.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480283/suppl/GSM480283.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480284 | GPL570 |
|
Subject 1188, Replicate 2
|
Derived from neonatal foreskin
|
subject/donor: 1188
gender: Male
tissue: cultured epidermis
replicate: 2
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480284
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480284/suppl/GSM480284.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480284/suppl/GSM480284.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480285 | GPL570 |
|
Subject 1188, Replicate 3
|
Derived from neonatal foreskin
|
subject/donor: 1188
gender: Male
tissue: cultured epidermis
replicate: 3
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480285
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480285/suppl/GSM480285.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480285/suppl/GSM480285.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480286 | GPL570 |
|
Subject 1188, Replicate 4
|
Derived from neonatal foreskin
|
subject/donor: 1188
gender: Male
tissue: cultured epidermis
replicate: 4
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480286
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480286/suppl/GSM480286.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480286/suppl/GSM480286.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480287 | GPL570 |
|
Subject 1188, Replicate 5
|
Derived from neonatal foreskin
|
subject/donor: 1188
gender: Male
tissue: cultured epidermis
replicate: 5
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480287
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480287/suppl/GSM480287.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480287/suppl/GSM480287.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480288 | GPL570 |
|
Subject 219, Replicate 1
|
Derived from neonatal foreskin
|
subject/donor: 219
gender: Male
tissue: cultured epidermis
replicate: 1
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480288
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480288/suppl/GSM480288.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480288/suppl/GSM480288.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480289 | GPL570 |
|
Subject 219, Replicate 2
|
Derived from neonatal foreskin
|
subject/donor: 219
gender: Male
tissue: cultured epidermis
replicate: 2
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480289
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480289/suppl/GSM480289.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480289/suppl/GSM480289.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480290 | GPL570 |
|
Subject 219, Replicate 3
|
Derived from neonatal foreskin
|
subject/donor: 219
gender: Male
tissue: cultured epidermis
replicate: 3
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480290
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480290/suppl/GSM480290.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480290/suppl/GSM480290.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480291 | GPL570 |
|
Subject 219, Replicate 4
|
Derived from neonatal foreskin
|
subject/donor: 219
gender: Male
tissue: cultured epidermis
replicate: 4
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480291
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480291/suppl/GSM480291.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480291/suppl/GSM480291.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480292 | GPL570 |
|
Subject 219, Replicate 5
|
Derived from neonatal foreskin
|
subject/donor: 219
gender: Male
tissue: cultured epidermis
replicate: 5
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480292
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480292/suppl/GSM480292.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480292/suppl/GSM480292.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480293 | GPL570 |
|
Subject 254, Replicate 1
|
Derived from neonatal foreskin
|
subject/donor: 254
gender: Male
tissue: cultured epidermis
replicate: 1
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480293
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480293/suppl/GSM480293.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480293/suppl/GSM480293.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480294 | GPL570 |
|
Subject 254, Replicate 2
|
Derived from neonatal foreskin
|
subject/donor: 254
gender: Male
tissue: cultured epidermis
replicate: 2
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480294
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480294/suppl/GSM480294.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480294/suppl/GSM480294.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480295 | GPL570 |
|
Subject 254, Replicate 3
|
Derived from neonatal foreskin
|
subject/donor: 254
gender: Male
tissue: cultured epidermis
replicate: 3
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480295
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480295/suppl/GSM480295.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480295/suppl/GSM480295.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480296 | GPL570 |
|
Subject 254, Replicate 4
|
Derived from neonatal foreskin
|
subject/donor: 254
gender: Male
tissue: cultured epidermis
replicate: 4
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480296
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480296/suppl/GSM480296.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480296/suppl/GSM480296.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480297 | GPL570 |
|
Subject 254, Replicate 5
|
Derived from neonatal foreskin
|
subject/donor: 254
gender: Male
tissue: cultured epidermis
replicate: 5
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480297
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480297/suppl/GSM480297.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480297/suppl/GSM480297.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480298 | GPL570 |
|
Subject 926, Replicate 1
|
Derived from neonatal foreskin
|
subject/donor: 926
gender: Male
tissue: cultured epidermis
replicate: 1
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480298
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480298/suppl/GSM480298.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480298/suppl/GSM480298.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480299 | GPL570 |
|
Subject 926, Replicate 2
|
Derived from neonatal foreskin
|
subject/donor: 926
gender: Male
tissue: cultured epidermis
replicate: 2
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480299
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480299/suppl/GSM480299.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480299/suppl/GSM480299.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480300 | GPL570 |
|
Subject 926, Replicate 3
|
Derived from neonatal foreskin
|
subject/donor: 926
gender: Male
tissue: cultured epidermis
replicate: 3
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480300
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480300/suppl/GSM480300.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480300/suppl/GSM480300.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480301 | GPL570 |
|
Subject 926, Replicate 4
|
Derived from neonatal foreskin
|
subject/donor: 926
gender: Male
tissue: cultured epidermis
replicate: 4
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480301
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480301/suppl/GSM480301.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480301/suppl/GSM480301.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
|
GSM480302 | GPL570 |
|
Subject 926, Replicate 5
|
Derived from neonatal foreskin
|
subject/donor: 926
gender: Male
tissue: cultured epidermis
replicate: 5
|
Gene expression data from reconstituted human epidermis
|
| Sample_geo_accession | GSM480302
| | Sample_status | Public on Jun 03 2010
| | Sample_submission_date | Dec 04 2009
| | Sample_last_update_date | Jun 02 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Following overnight incubation in MMM Media, individual epidermal tissues were peeled from the tissue insert and placed into 2 ml of RNAlater® (Ambion/ABI, Foster City, CA) pre-loaded into a 24-well plate. After 24 hours incubation under 4°C, the RNAlater® submersed tissues were transferred into a 2 ml Seal-Rite® microcentrifuge tube (USA Scientific Inc, Ocala, FL) and all excess RNAlater® was removed from the tissue by gentle pipetting. The tissues were stored at -80°C until RNA was isolated.
| | Sample_growth_protocol_ch1 | Reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926) were acquired from MatTek Corporation (Ashland, MA). Five tissues from each donor were placed into 6-well plates with 1ml/tissue/well of pre-warmed (37°C) NMM media (MatTek Corp.) and incubated overnight at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRI-Reagent and protocol provided by supplier (Molecular Research Center, Cincinnati, OH). Extracted RNA was further purified using Qiagen RNEasy columns and protocol provided by supplier (QIAgen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was converted to biotin-labeled cRNA GeneChip target using Ambion Message Amp II- Biotin Enhanced Kit according to manufacture's recommendations. Biotin-labeled cRNA was purified using the Qiagen RNeasy kit and a total of 20 μg of cRNA was fragmented randomly to ~200 bp at 94°C for 35 min (200 mM Tris-acetate, pH 8.2, 500 mM KOAc, 150 mM MgOAc).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Human U133plus2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jay,Patrick,Tiesman
| | Sample_contact_laboratory | Miami Valley Innovation Center
| | Sample_contact_department | Global Biotechnology
| | Sample_contact_institute | Procter & Gamble
| | Sample_contact_address | PO Box 538707
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45253-8707
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480302/suppl/GSM480302.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480302/suppl/GSM480302.CHP.gz
| | Sample_series_id | GSE19330
| | Sample_data_row_count | 54675
| | |
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