Result

Search results for the GEO ID: GSE19339

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM480382

GPL570

blood vessel, infarction, biological rep1

blood vessel after myocardial infarction

disease state: myocardial infarction tissue: blood vessel cell type: leukocytes

Gene expression data. infarction_1

Sample_geo_accession	GSM480382
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480382/suppl/GSM480382.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480383

GPL570

blood vessel, infarction, biological rep2

blood vessel after myocardial infarction

disease state: myocardial infarction tissue: blood vessel cell type: leukocytes

Gene expression data. infarction_2

Sample_geo_accession	GSM480383
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480383/suppl/GSM480383.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480384

GPL570

blood vessel, infarction, biological rep3

blood vessel after myocardial infarction

disease state: myocardial infarction tissue: blood vessel cell type: leukocytes

Gene expression data. infarction_3

Sample_geo_accession	GSM480384
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480384/suppl/GSM480384.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480385

GPL570

blood vessel, infarction, biological rep4

blood vessel after myocardial infarction

disease state: myocardial infarction tissue: blood vessel cell type: leukocytes

Gene expression data. infarction_4

Sample_geo_accession	GSM480385
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480385/suppl/GSM480385.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480386

GPL570

blood, normal, biological rep1

blood

disease state: normal tissue: blood cell type: leukocytes

Gene expression data. normal_1

Sample_geo_accession	GSM480386
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480386/suppl/GSM480386.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480387

GPL570

blood, normal, biological rep2

blood

disease state: normal tissue: blood cell type: leukocytes

Gene expression data. normal_2

Sample_geo_accession	GSM480387
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480387/suppl/GSM480387.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480388

GPL570

blood, normal, biological rep3

blood

disease state: normal tissue: blood cell type: leukocytes

Gene expression data. normal_3

Sample_geo_accession	GSM480388
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480388/suppl/GSM480388.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

GSM480389

GPL570

blood, normal, biological rep4

blood

disease state: normal tissue: blood cell type: leukocytes

Gene expression data. normal_4

Sample_geo_accession	GSM480389
Sample_status	Public on Dec 04 2011
Sample_submission_date	Dec 04 2009
Sample_last_update_date	Dec 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Experiments were performed with material in the peripheral blood and at the site of coronary occlusion obtained from 4 patients undergoing primary percutaneous coronary intervention (PCI) for ACS, presenting clinically as ST-segment elevation myocardial infarctions (STEMI). Immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus, the culprit coronary artery was intubated with a 6 F Medtronic guiding catheter and wired with a 0.014 inch guide wire. Prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter (Export™XT6F Aspiration Catheter, Medronic Inc., Minneapolis, MN, USA) and immediately immersed in phosphate-buffered saline (PBS) containing vials. In parallel, arterial blood was sampled from the aorta and stored in vials. In addition, peripheral blood from healthy controls was obtained in a similar manner. Peripheral blood leukocytes (PBL) were treated in a similar fashion and mRNA was extracted from both cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA of freshly isolated thrombi and corresponding peripheral blood leukocytes was isolated using the commercially available QIAGEN RNeasy Mini Kit (QIAGEN) in accordance with the manufacturer’s recommendations.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Single-stranded cDNA was fragmented and labeled with biotin using FL-Ovation Biotin Module V2 (NuGEN).
Sample_hyb_protocol	Biotin-labeled single-stranded cDNA was used for hybridization to GeneChip Human Genome U133 Plus 2.0 arrays for 18h at 45°C. Arrays were then washed using an Affymetrix Fluidics Station 450.
Sample_scan_protocol	An Affymetrix GeneChip Scanner 3000 was used to measure the fluorescent intensity emitted by the labeled targets. Generated raw data were processed using the Affymetrix AGCC software.
Sample_data_processing	Data was RMA processed in R with the Affy package.
Sample_platform_id	GPL570
Sample_contact_name	Stefan,,Zoller
Sample_contact_email	szoller@env.ethz.ch
Sample_contact_department	Genetic Diversity Centre
Sample_contact_institute	ETH Zurich
Sample_contact_address	Universitatstrasse 16
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8092
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM480nnn/GSM480389/suppl/GSM480389.CEL.gz
Sample_series_id	GSE19339
Sample_data_row_count	54675

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