Search results for the GEO ID: GSE19393  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM481210 | GPL570 |
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primary sc preadipocytes.48h CNTRL.replicate 1
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Primary preadipocytes; vehicle control (EtOH) - 48 hours
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cell type: Cultured primary preadipocytes
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gene expression data from primary preadipocytes treated with vehicle (EtOH) for 48 hours
|
| Sample_geo_accession | GSM481210
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Dec 11 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent preadipocytes (12-well dish) were stimulated with vehicle (EtOH) or 1uM dex for 48 hours in growth media (above) containing 3% calf serum.
| | Sample_growth_protocol_ch1 | Human subcutaneous primary preadipocytes were purchased from Zen-Bio Inc. Preadipocytes from 5 female donors (average BMI 22.5±0.2kg/m2) were pooled prior to the initial seeding in Nunc brand T75 flasks. Cells were maintained at 5% CO2 in DMEM with 1.0g/L glucose, 20% calf serum, 100U/ml penicillin, 100mg/ml streptomycin and 50U/ml nystatin. Media was replaced every 2 days. Cells were expanded once (1:2 dilution) prior to seeding in Nunc-brand 12-well dishes at which point they were allowed to reach confluence (occurred within 24h) and were treated as described.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the Qiagen Rneasy Mini Kit as per manufacturer's instuctions. For each sample, 3 wells of the 12-well dish were pooled. The quality of the samples was verified using the Agilent Bioanalyzer 2100 (Nano Chips).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Samples were processed and hybridized accoring to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Normalized expression data has been produced by Robust Multichip Average (RMA) using the software ArrayAssist from Stratagene (originally Iobion). Log2 expression values are automatically unlogged by this software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Haché
| | Sample_contact_email | rjhache@ucalgary.ca
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 2500 University Drive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | Alberta
| | Sample_contact_zip/postal_code | T2N 1N4
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM481nnn/GSM481210/suppl/GSM481210.CEL.gz
| | Sample_series_id | GSE19393
| | Sample_data_row_count | 54675
| | |
|
GSM481211 | GPL570 |
|
primary sc preadipocytes.48h CNTRL.replicate 2
|
Primary preadipocytes; vehicle control (EtOH) - 48 hours
|
cell type: Cultured primary preadipocytes
|
gene expression data from primary preadipocytes treated with vehicle (EtOH) for 48 hours
|
| Sample_geo_accession | GSM481211
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Dec 11 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent preadipocytes (12-well dish) were stimulated with vehicle (EtOH) or 1uM dex for 48 hours in growth media (above) containing 3% calf serum.
| | Sample_growth_protocol_ch1 | Human subcutaneous primary preadipocytes were purchased from Zen-Bio Inc. Preadipocytes from 5 female donors (average BMI 22.5±0.2kg/m2) were pooled prior to the initial seeding in Nunc brand T75 flasks. Cells were maintained at 5% CO2 in DMEM with 1.0g/L glucose, 20% calf serum, 100U/ml penicillin, 100mg/ml streptomycin and 50U/ml nystatin. Media was replaced every 2 days. Cells were expanded once (1:2 dilution) prior to seeding in Nunc-brand 12-well dishes at which point they were allowed to reach confluence (occurred within 24h) and were treated as described.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the Qiagen Rneasy Mini Kit as per manufacturer's instuctions. For each sample, 3 wells of the 12-well dish were pooled. The quality of the samples was verified using the Agilent Bioanalyzer 2100 (Nano Chips).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Samples were processed and hybridized accoring to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Normalized expression data has been produced by Robust Multichip Average (RMA) using the software ArrayAssist from Stratagene (originally Iobion). Log2 expression values are automatically unlogged by this software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Haché
| | Sample_contact_email | rjhache@ucalgary.ca
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 2500 University Drive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | Alberta
| | Sample_contact_zip/postal_code | T2N 1N4
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM481nnn/GSM481211/suppl/GSM481211.CEL.gz
| | Sample_series_id | GSE19393
| | Sample_data_row_count | 54675
| | |
|
GSM481212 | GPL570 |
|
primary sc preadipocytes.48h DEX.replicate 1
|
Primary preadipocytes; DEX treated (1uM) - 48 hours
|
cell type: Cultured primary preadipocytes
|
gene expression data from primary preadipocytes treated with 1uM DEX for 48 hours
|
| Sample_geo_accession | GSM481212
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Dec 11 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent preadipocytes (12-well dish) were stimulated with vehicle (EtOH) or 1uM dex for 48 hours in growth media (above) containing 3% calf serum.
| | Sample_growth_protocol_ch1 | Human subcutaneous primary preadipocytes were purchased from Zen-Bio Inc. Preadipocytes from 5 female donors (average BMI 22.5±0.2kg/m2) were pooled prior to the initial seeding in Nunc brand T75 flasks. Cells were maintained at 5% CO2 in DMEM with 1.0g/L glucose, 20% calf serum, 100U/ml penicillin, 100mg/ml streptomycin and 50U/ml nystatin. Media was replaced every 2 days. Cells were expanded once (1:2 dilution) prior to seeding in Nunc-brand 12-well dishes at which point they were allowed to reach confluence (occurred within 24h) and were treated as described.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the Qiagen Rneasy Mini Kit as per manufacturer's instuctions. For each sample, 3 wells of the 12-well dish were pooled. The quality of the samples was verified using the Agilent Bioanalyzer 2100 (Nano Chips).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Samples were processed and hybridized accoring to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Normalized expression data has been produced by Robust Multichip Average (RMA) using the software ArrayAssist from Stratagene (originally Iobion). Log2 expression values are automatically unlogged by this software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Haché
| | Sample_contact_email | rjhache@ucalgary.ca
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 2500 University Drive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | Alberta
| | Sample_contact_zip/postal_code | T2N 1N4
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM481nnn/GSM481212/suppl/GSM481212.CEL.gz
| | Sample_series_id | GSE19393
| | Sample_data_row_count | 54675
| | |
|
GSM481213 | GPL570 |
|
primary sc preadipocytes.48h DEX.replicate 2
|
Primary preadipocytes; DEX treated (1uM) - 48 hours
|
cell type: Cultured primary preadipocytes
|
gene expression data from primary preadipocytes treated with 1uM DEX for 48 hours
|
| Sample_geo_accession | GSM481213
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Dec 11 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent preadipocytes (12-well dish) were stimulated with vehicle (EtOH) or 1uM dex for 48 hours in growth media (above) containing 3% calf serum.
| | Sample_growth_protocol_ch1 | Human subcutaneous primary preadipocytes were purchased from Zen-Bio Inc. Preadipocytes from 5 female donors (average BMI 22.5±0.2kg/m2) were pooled prior to the initial seeding in Nunc brand T75 flasks. Cells were maintained at 5% CO2 in DMEM with 1.0g/L glucose, 20% calf serum, 100U/ml penicillin, 100mg/ml streptomycin and 50U/ml nystatin. Media was replaced every 2 days. Cells were expanded once (1:2 dilution) prior to seeding in Nunc-brand 12-well dishes at which point they were allowed to reach confluence (occurred within 24h) and were treated as described.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the Qiagen Rneasy Mini Kit as per manufacturer's instuctions. For each sample, 3 wells of the 12-well dish were pooled. The quality of the samples was verified using the Agilent Bioanalyzer 2100 (Nano Chips).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Samples were processed and hybridized accoring to the standard small sample protocol from Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Normalized expression data has been produced by Robust Multichip Average (RMA) using the software ArrayAssist from Stratagene (originally Iobion). Log2 expression values are automatically unlogged by this software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Haché
| | Sample_contact_email | rjhache@ucalgary.ca
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 2500 University Drive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | Alberta
| | Sample_contact_zip/postal_code | T2N 1N4
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM481nnn/GSM481213/suppl/GSM481213.CEL.gz
| | Sample_series_id | GSE19393
| | Sample_data_row_count | 54675
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