Search results for the GEO ID: GSE19403  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM482060 | GPL1261 |
|
spinal cord wild type at 15 days, biological replicate 1
|
ventrolateral spinal cord white matter, wild type at 15 days
|
genotype: Wild type
developmental stage: Day 15
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482060
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482060/suppl/GSM482060.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482061 | GPL1261 |
|
spinal cord wild type at 15 days, biological replicate 2
|
ventrolateral spinal cord white matter, wild type at 15 days
|
genotype: Wild type
developmental stage: Day 15
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482061
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482061/suppl/GSM482061.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482062 | GPL1261 |
|
spinal cord wild type at 15 days, biological replicate 3
|
ventrolateral spinal cord white matter, wild type at 15 days
|
genotype: Wild type
developmental stage: Day 15
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482062
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482062/suppl/GSM482062.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482063 | GPL1261 |
|
spinal cord Olig2cre/DA-Cat at 15 days, biological replicate 1
|
ventrolateral spinal cord white matter, Olig2cre/DA-Cat at 15 days
|
genotype: Olig2cre/DA-Cat
developmental stage: Day 15
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482063
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482063/suppl/GSM482063.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482064 | GPL1261 |
|
spinal cord Olig2cre/DA-Cat at 15 days, biological replicate 2
|
ventrolateral spinal cord white matter, Olig2cre/DA-Cat at 15 days
|
genotype: Olig2cre/DA-Cat
developmental stage: Day 15
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482064
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482064/suppl/GSM482064.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482065 | GPL1261 |
|
spinal cord Olig2cre/DA-Cat at 15 days, biological replicate 3
|
ventrolateral spinal cord white matter, Olig2cre/DA-Cat at 15 days
|
genotype: Olig2cre/DA-Cat
developmental stage: Day 15
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482065
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482065/suppl/GSM482065.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482066 | GPL1261 |
|
spinal cord wild type at 4 days, biological replicate 1
|
ventrolateral spinal cord white matter, wild type at 4 days
|
genotype: Wild type
developmental stage: Day 4
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482066
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482066/suppl/GSM482066.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482067 | GPL1261 |
|
spinal cord wild type at 4 days, biological replicate 2
|
ventrolateral spinal cord white matter, wild type at 4 days
|
genotype: Wild type
developmental stage: Day 4
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482067
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482067/suppl/GSM482067.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482068 | GPL1261 |
|
spinal cord wild type at 4 days, biological replicate 3
|
ventrolateral spinal cord white matter, wild type at 4 days
|
genotype: Wild type
developmental stage: Day 4
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482068
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482068/suppl/GSM482068.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482069 | GPL1261 |
|
spinal cord Olig2cre/DA-Cat at 4 days, biological replicate 1
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ventrolateral spinal cord white matter, Olig2cre/DA-Cat at 4 days
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genotype: Olig2cre/DA-Cat
developmental stage: Day 4
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ventrolateral spinal cord white matter was injected with 1% lysolecithin
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| Sample_geo_accession | GSM482069
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482069/suppl/GSM482069.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482070 | GPL1261 |
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spinal cord Olig2cre/DA-Cat at 4 days, biological replicate 2
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ventrolateral spinal cord white matter, Olig2cre/DA-Cat at 4 days
|
genotype: Olig2cre/DA-Cat
developmental stage: Day 4
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482070
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482070/suppl/GSM482070.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
| | |
|
GSM482071 | GPL1261 |
|
spinal cord Olig2cre/DA-Cat at 4 days, biological replicate 3
|
ventrolateral spinal cord white matter, Olig2cre/DA-Cat at 4 days
|
genotype: Olig2cre/DA-Cat
developmental stage: Day 4
|
ventrolateral spinal cord white matter was injected with 1% lysolecithin
|
| Sample_geo_accession | GSM482071
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | Dec 09 2009
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Demyelinated lesions were produced in the ventrolateral spinal cord white matter of 8- to 10-wk-old Olig2cre/DA-Cat, and wild-type littermate mice. Anesthesia was induced and maintained with inhalational isoflurane/oxygen, supplemented with 0.05 mL of buprenorphine (Vetergesic 0.05 mg/mL) given subcutaneously. Having exposed the spinal vertebrae at the level of T12/T13, tissue was cleared overlying the intervertebral space, and the dura was pierced with a dental needle just lateral to midline. A Hamilton needle was advanced through the pierced dura at an angle of 45° and 0.5 μL of 1% lysolecithin (L-α-lysophosphatidylcholine) was injected into the ventrolateral white matter. Unlesioned spinal cords or 14-dpl lesioned cords were removed fresh and snap-frozen in iso-pentane and stored at −80°C. Cords were subsequently cut as 50-μm-thick cryosections, and unlesioned white matter or 14-dpl lesions were manually isolated from the sections using a micro-scalpel (Fine Science Tools 10318-14). Approximately 80 sections were cut from each spinal cord to obtain 1 μg of RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAqueous-Micro kit (Ambion), and RNA quality was checked on an Agilent Bioanalyser.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix 3' IVT
| | Sample_hyb_protocol | Affymetrix 3' IVT
| | Sample_scan_protocol | Affymetrix 3' IVT
| | Sample_data_processing | BRB array Tools
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lohith,Reddy,Madireddy
| | Sample_contact_email | lohithreddym@gmail.com
| | Sample_contact_laboratory | Multiple Sclerosis Research Group
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | 513 Parnassus Ave, Medical Science Building Room 258
| | Sample_contact_city | SAN FRANCISCO
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482071/suppl/GSM482071.CEL.gz
| | Sample_series_id | GSE19403
| | Sample_data_row_count | 45101
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