Result

Search results for the GEO ID: GSE19406

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM482707

GPL570

FADU-cDNA3

human hypopharyngeal cancer cell line FADU transfected with pcDNA3.1 empty vector

cell line source ethnicity: Caucasian cell line source gender: Male cell line source age: 56 cell line source tissue: primary human hypopharyngeal squamous cell carcinoma transfection: pcDNA3.1 empty vector cell line: FADU

Human hypopharyngeal cancer cell line FADU transfected with pcDNA3.1 empty vector were grown to near confluence and collected total RNA for array analysis.

Sample_geo_accession	GSM482707
Sample_status	Public on Dec 10 2010
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	cultivated in RPMI + 10% fetal bovine serum
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_hyb_protocol	Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_scan_protocol	Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
Sample_data_processing	We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each probe sets on the U133 2.0 oligonucleotide arrays.
Sample_platform_id	GPL570
Sample_contact_name	Muh-Hwa,,Yang
Sample_contact_email	mhyang2@vghtpe.gov.tw
Sample_contact_phone	+886-228235870
Sample_contact_fax	+886-228235870
Sample_contact_laboratory	Dr. M.H. Yang's Lab
Sample_contact_department	Institute of Clinical Medicine
Sample_contact_institute	National Yang-Ming University
Sample_contact_address	No.155, Sec.2, Li-Nong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482707/suppl/GSM482707_H0111_004_FADU_cDNA3.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482707/suppl/GSM482707_H0111_004_FADUcDNA3.CEL.gz
Sample_series_id	GSE19406
Sample_data_row_count	54675

GSM482708

GPL570

FADU-HIF1a(dODD)

human hypopharyngeal squamous cell carcinoma cell line FADU transfected with pHA-HIF1a(dODD)

Human hypopharyngeal cancer cell line FADU transfected with pHA-HIF1a(dODD) vector which expresses a HIF-1a mutant with an oxygen degradation domain (∆ODD) deletion that functions in a normoxic environment were grown to near confluence and collected total RNA for array analysis.

Sample_geo_accession	GSM482708
Sample_status	Public on Dec 10 2010
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	cultivated in RPMI + 10% fetal bovine serum
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_hyb_protocol	Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_scan_protocol	Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
Sample_data_processing	We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each probe sets on the U133 2.0 oligonucleotide arrays.
Sample_platform_id	GPL570
Sample_contact_name	Muh-Hwa,,Yang
Sample_contact_email	mhyang2@vghtpe.gov.tw
Sample_contact_phone	+886-228235870
Sample_contact_fax	+886-228235870
Sample_contact_laboratory	Dr. M.H. Yang's Lab
Sample_contact_department	Institute of Clinical Medicine
Sample_contact_institute	National Yang-Ming University
Sample_contact_address	No.155, Sec.2, Li-Nong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482708/suppl/GSM482708_H0111_005_FADU_HIF1a_dODD_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482708/suppl/GSM482708_H0111_005_FADU_HIF1a_dODD_.CHP.gz
Sample_series_id	GSE19406
Sample_data_row_count	54675

GSM482709

GPL570

FADU-Twist1

human hypopharyngeal squamous cell carcinoma cell line FADU transfected with pFLAG-Twist1

Human hypopharyngeal cancer cell line FADU transfected with the Twist1 expression vector pFLAG-Twist1 were grown to near confluence and collected total RNA for array analysis.

Sample_geo_accession	GSM482709
Sample_status	Public on Dec 10 2010
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	cultivated in RPMI + 10% fetal bovine serum
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_hyb_protocol	Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_scan_protocol	Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
Sample_data_processing	We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each probe sets on the U133 2.0 oligonucleotide arrays.
Sample_platform_id	GPL570
Sample_contact_name	Muh-Hwa,,Yang
Sample_contact_email	mhyang2@vghtpe.gov.tw
Sample_contact_phone	+886-228235870
Sample_contact_fax	+886-228235870
Sample_contact_laboratory	Dr. M.H. Yang's Lab
Sample_contact_department	Institute of Clinical Medicine
Sample_contact_institute	National Yang-Ming University
Sample_contact_address	No.155, Sec.2, Li-Nong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482709/suppl/GSM482709_H0111_006_FADU_Twist1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482709/suppl/GSM482709_H0111_006_FADU_Twist1.CHP.gz
Sample_series_id	GSE19406
Sample_data_row_count	54675

GSM482710

GPL570

FADU-Bmi1

human hypopharyngeal squamous cell carcinoma cell line FADU transfected with pcDNA3-Bmi1

cell line source ethnicity: Caucasian cell line source gender: Male cell line source age: 56 cell line source tissue: primary human hypopharyngeal squamous cell carcinoma p transfection: cDNA3-Bmi1 cell line: FADU

Human hypopharyngeal cancer cell line FADU transfected with the Bmi1 expression vector pcDNA3-Bmi1 were grown to near confluence and collected total RNA for array analysis.

Sample_geo_accession	GSM482710
Sample_status	Public on Dec 10 2010
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	cultivated in RPMI + 10% fetal bovine serum
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were lysed by adding 2 ml of trizol reagent (Invitrogen). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_hyb_protocol	Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_scan_protocol	Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
Sample_data_processing	We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each probe sets on the U133 2.0 oligonucleotide arrays.
Sample_platform_id	GPL570
Sample_contact_name	Muh-Hwa,,Yang
Sample_contact_email	mhyang2@vghtpe.gov.tw
Sample_contact_phone	+886-228235870
Sample_contact_fax	+886-228235870
Sample_contact_laboratory	Dr. M.H. Yang's Lab
Sample_contact_department	Institute of Clinical Medicine
Sample_contact_institute	National Yang-Ming University
Sample_contact_address	No.155, Sec.2, Li-Nong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482710/suppl/GSM482710_H0111_007_FADU_Bmi1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482710/suppl/GSM482710_H0111_007_FADU_Bmi1.CHP.gz
Sample_series_id	GSE19406
Sample_data_row_count	54675

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