Result

Search results for the GEO ID: GSE19408

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GSM ID

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Title

Source name

Description

Characteristics

GSM482745

GPL1261

whole uterus, oil control for 6 hr, rep1

Mouse uterus treated with oil for 6 hrs, total RNA

tissue: whole uterus agent: oil

Gene expression data from uterus treated oil or ICI 182,780 Mutant Oil-1

Sample_geo_accession	GSM482745
Sample_status	Public on Dec 10 2009
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	ovariectomized mice were treated with 3 daily injections of 100 ng of estrodial (E2) per mouse. After 2 days of rest, mice were then treated with daily injections of 1 mg of progesterone (P4) and 6.7 ng of E2 per mouse for 2 days, subcutaneously (s.c.). Oil or ICI 182,780 was treated at 34 hr of eP treatment for 6 hrs.
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy kit (Qiagen) was used for extraction of total RNA.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Affymetrix mouse genome 430 2.0 arrays
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Dong-Kee,,Lee
Sample_contact_email	dongkeel@bcm.tmc.edu
Sample_contact_phone	7137986252
Sample_contact_department	Molecular and Cellular Biology
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482745/suppl/GSM482745.cel.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482745/suppl/GSM482745.chp.gz
Sample_series_id	GSE19408
Sample_data_row_count	45101

GSM482746

GPL1261

whole uterus, oil control for 6 hr, rep2

Mouse uterus treated with oil for 6 hrs, total RNA

tissue: whole uterus agent: oil

Gene expression data from uterus treated oil or ICI 182,780 Mutant Oil-2

Sample_geo_accession	GSM482746
Sample_status	Public on Dec 10 2009
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	ovariectomized mice were treated with 3 daily injections of 100 ng of estrodial (E2) per mouse. After 2 days of rest, mice were then treated with daily injections of 1 mg of progesterone (P4) and 6.7 ng of E2 per mouse for 2 days, subcutaneously (s.c.). Oil or ICI 182,780 was treated at 34 hr of eP treatment for 6 hrs.
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy kit (Qiagen) was used for extraction of total RNA.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Affymetrix mouse genome 430 2.0 arrays
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Dong-Kee,,Lee
Sample_contact_email	dongkeel@bcm.tmc.edu
Sample_contact_phone	7137986252
Sample_contact_department	Molecular and Cellular Biology
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482746/suppl/GSM482746.cel.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482746/suppl/GSM482746.chp.gz
Sample_series_id	GSE19408
Sample_data_row_count	45101

GSM482747

GPL1261

whole uterus, ICI treated for 6 hr, rep1

Mouse uterus treated with ICI for 6 hrs, total RNA

tissue: whole uterus agent: ICI

Gene expression data from uterus treated oil or ICI 182,780 Mutant ICI-1

Sample_geo_accession	GSM482747
Sample_status	Public on Dec 10 2009
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	ovariectomized mice were treated with 3 daily injections of 100 ng of estrodial (E2) per mouse. After 2 days of rest, mice were then treated with daily injections of 1 mg of progesterone (P4) and 6.7 ng of E2 per mouse for 2 days, subcutaneously (s.c.). Oil or ICI 182,780 was treated at 34 hr of eP treatment for 6 hrs.
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy kit (Qiagen) was used for extraction of total RNA.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Affymetrix mouse genome 430 2.0 arrays
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Dong-Kee,,Lee
Sample_contact_email	dongkeel@bcm.tmc.edu
Sample_contact_phone	7137986252
Sample_contact_department	Molecular and Cellular Biology
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482747/suppl/GSM482747.cel.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482747/suppl/GSM482747.chp.gz
Sample_series_id	GSE19408
Sample_data_row_count	45101

GSM482748

GPL1261

whole uterus, ICI treated for 6 hr, rep2

Mouse uterus treated with ICI for 6 hrs, total RNA

tissue: whole uterus agent: ICI

Gene expression data from uterus treated oil or ICI 182,780 Mutant ICI-2

Sample_geo_accession	GSM482748
Sample_status	Public on Dec 10 2009
Sample_submission_date	Dec 10 2009
Sample_last_update_date	Dec 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	ovariectomized mice were treated with 3 daily injections of 100 ng of estrodial (E2) per mouse. After 2 days of rest, mice were then treated with daily injections of 1 mg of progesterone (P4) and 6.7 ng of E2 per mouse for 2 days, subcutaneously (s.c.). Oil or ICI 182,780 was treated at 34 hr of eP treatment for 6 hrs.
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy kit (Qiagen) was used for extraction of total RNA.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA .
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Affymetrix mouse genome 430 2.0 arrays
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Dong-Kee,,Lee
Sample_contact_email	dongkeel@bcm.tmc.edu
Sample_contact_phone	7137986252
Sample_contact_department	Molecular and Cellular Biology
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482748/suppl/GSM482748.cel.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482748/suppl/GSM482748.chp.gz
Sample_series_id	GSE19408
Sample_data_row_count	45101

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