Search results for the GEO ID: GSE19474  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM485238 | GPL1261 |
|
rostral 5HT neurons (R+) biological replicate 1
|
rostral 5HT neurons (R+)
|
tissue: rostral 5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den09
|
| Sample_geo_accession | GSM485238
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485238/suppl/GSM485238.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485239 | GPL1261 |
|
rostral non-5HT neurons (R-) biological replicate 1
|
rostral non-5HT neurons (R-)
|
tissue: rostral non-5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den10
|
| Sample_geo_accession | GSM485239
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485239/suppl/GSM485239.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485240 | GPL1261 |
|
caudal 5HT neurons (C+)biological replicate 1
|
caudal 5HT neurons (C+)
|
tissue: caudal 5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den11
|
| Sample_geo_accession | GSM485240
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485240/suppl/GSM485240.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485241 | GPL1261 |
|
caudal non-5HT neurons (C-)biological replicate 1
|
caudal non-5HT neurons (C-)
|
tissue: caudal non-5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den12
|
| Sample_geo_accession | GSM485241
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485241/suppl/GSM485241.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485242 | GPL1261 |
|
rostral 5HT neurons (R+) biological replicate 2
|
rostral 5HT neurons (R+)
|
tissue: rostral 5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den13
|
| Sample_geo_accession | GSM485242
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485242/suppl/GSM485242.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485243 | GPL1261 |
|
rostral non-5HT neurons (R-)biological replicate 2
|
rostral non-5HT neurons (R-)
|
tissue: rostral non-5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den14
|
| Sample_geo_accession | GSM485243
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485243/suppl/GSM485243.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485244 | GPL1261 |
|
caudal 5HT neurons (C+) biological replicate 2
|
caudal 5HT neurons (C+)
|
tissue: caudal 5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den15
|
| Sample_geo_accession | GSM485244
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485244/suppl/GSM485244.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485245 | GPL1261 |
|
caudal non-5HT neurons (C-) biological replicate 2
|
caudal non-5HT neurons (C-)
|
tissue: caudal non-5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den16
|
| Sample_geo_accession | GSM485245
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485245/suppl/GSM485245.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485246 | GPL1261 |
|
rostral 5HT neurons (R+) biological replicate 3
|
rostral 5HT neurons (R+)
|
tissue: rostral 5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den17
|
| Sample_geo_accession | GSM485246
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485246/suppl/GSM485246.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485247 | GPL1261 |
|
rostral non-5HT neurons (R-) biological replicate 3
|
rostral non-5HT neurons (R-)
|
tissue: rostral non-5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den18
|
| Sample_geo_accession | GSM485247
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485247/suppl/GSM485247.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485248 | GPL1261 |
|
caudal 5HT neurons (C+) biological replicate 3
|
caudal 5HT neurons (C+)
|
tissue: caudal 5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den19
|
| Sample_geo_accession | GSM485248
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485248/suppl/GSM485248.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
|
GSM485249 | GPL1261 |
|
caudal non-5HT neurons (C-) biological replicate 3
|
caudal non-5HT neurons (C-)
|
tissue: caudal non-5HT neurons
genotype: ePet-EYFP
age: E12.5
|
gene expression data from rostral 5HT neurons (R+), rostral non-5HT neurons (R-), caudal 5HT neurons (C+), and caudal non-5HT neurons (C-)
61 den20
|
| Sample_geo_accession | GSM485249
| | Sample_status | Public on Dec 16 2009
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated by mild trypsin and mechanical trituration with a glass pipette into single cell suspensions. Cells were sorted and 4 populations collected directly into Trizol: (R+. R-, C+, and C).
| | Sample_growth_protocol_ch1 | E12.5 ePet-EYFP neural tubes were dissected into rostral and caudal portions by making incisions at the pontine flexure, mesecephalic flexure, and spinal cord.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200,000 sorted cells for reach replicate using one round of amplication.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix High Density Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Evan,S.,Deneris
| | Sample_contact_email | esd@case.edu
| | Sample_contact_phone | 216 368 8725
| | Sample_contact_fax | 216 368 4650
| | Sample_contact_laboratory | E638
| | Sample_contact_department | Neurosciences
| | Sample_contact_institute | Case Western Reserve University
| | Sample_contact_address | 2109 Adlebert Road
| | Sample_contact_city | Cleveland
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 44106
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM485nnn/GSM485249/suppl/GSM485249.CEL.gz
| | Sample_series_id | GSE19474
| | Sample_data_row_count | 45101
| | |
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