Result

Search results for the GEO ID: GSE19495

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM486221

GPL570

control-1, cells cultured in regular DMEM

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none

Sample_geo_accession	GSM486221
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486221/suppl/GSM486221.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486222

GPL570

control-2, cells cultured in regular DMEM

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486222
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486222/suppl/GSM486222.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486223

GPL570

control-3, cells cultured in regular DMEM

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486223
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486223/suppl/GSM486223.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486224

GPL570

control-4, cells cultured in regular DMEM

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486224
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486224/suppl/GSM486224.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486225

GPL570

Treatment-1, cells cultured in DMEM plus 5 mM HisOH

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486225
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium. For treatment, cells were cultured in DMEM medium containing 5 mM HisOH for 4 hours.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486225/suppl/GSM486225.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486226

GPL570

Treatment-2, cells cultured in DMEM plus 5 mM HisOH

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486226
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium. For treatment, cells were cultured in DMEM medium containing 5 mM HisOH for 4 hours.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486226/suppl/GSM486226.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486227

GPL570

Treatment-3, cells cultured in DMEM plus 5 mM HisOH

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486227
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium. For treatment, cells were cultured in DMEM medium containing 5 mM HisOH for 4 hours.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486227/suppl/GSM486227.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

GSM486228

GPL570

Treatment-4, cells cultured in DMEM plus 5 mM HisOH

Human Hepatoma Cells

cell type: Human Hepatoma Cells

none none

Sample_geo_accession	GSM486228
Sample_status	Public on Dec 20 2009
Sample_submission_date	Dec 15 2009
Sample_last_update_date	Dec 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	12 hours prior to treatement, cells were replenished with fresh medium. For treatment, cells were cultured in DMEM medium containing 5 mM HisOH for 4 hours.
Sample_growth_protocol_ch1	Human Heptoma cells were cultured in DMEM plus 10 % FBS
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy kit (Qiagen), including DNase I treatment before the final elution to eliminate any DNA contamination
Sample_label_ch1	biotin
Sample_label_protocol_ch1	A 200 ng aliquot of total RNA from four independent incubations for each condition was labeled using the Affymetrix GeneChip® 3' IVT Express Kit.
Sample_hyb_protocol	Biotin-labeled cRNA was fragmented for 35 min at 94°C. This fragmented cRNA (0.05 μg/μl) was then mixed with the control oligonucleotide B2 (50 pM), 20X eukaryotic hybridization controls (BioB at 1.5 pM, BioC at 5 pM, BioDn at 25 pM, and CreX at 100 pM, Affymetrix 900454), herring sperm DNA (0.1 mg/ml, Promega), acetylated BSA (50 mg/ml, GIBCO-BRL), and 2X hybridization buffer. The reaction was heated to 99°C for 5 min, then transferred to 45°C for 5 min, and finally centrifuged at 15,000 x g in a microcentrifuge for 5 min. A 200μl aliquot was injected into a Human Genome U133 Plus 2.0 Array (Affymetrix) representing over 47,000 mRNA species and 38,500 known human genes. Hybridization was completed in a 45°C oven for 16 h. The solution was removed and the array was washed and stained using the Euk-WS2 fluidics protocol and the streptavidin-phycoerythrin reagent (Affymetrix).
Sample_scan_protocol	The Genechips were scanned with an Affymetrix G7 scanner
Sample_data_processing	Microarray data were normalized and a model-based expression matrix was derived using the perfect-match-only algorithms of dChip (Li and Wong 31-36). For unsupervised analysis, probe sets for which the hybridization signal intensity varied across the data set with a coefficient of variation of >0.5 were identified and visualized by average linkage hierarchal clustering using the clustering algorithms implemented in dChip.
Sample_platform_id	GPL570
Sample_contact_name	jixiu,,shan
Sample_contact_laboratory	Dr. Michael Kilberg
Sample_contact_department	Biochemistry and Molecular Biology
Sample_contact_institute	University of Florida
Sample_contact_address	1600 SW Archer Road
Sample_contact_city	Gainesville
Sample_contact_state	FL
Sample_contact_zip/postal_code	32610
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486228/suppl/GSM486228.CEL.gz
Sample_series_id	GSE19495
Sample_data_row_count	54675

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