Search results for the GEO ID: GSE19502  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM486405 | GPL1261 |
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Myc Hi R1
|
Total RNA, LSK, c-Myc-eGFP Hi
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strain: WT B6/129
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WT Lineage negative, c-Kit and Sca1 (LSK) positive c-Myc eGFP Hi expressing bone marrow progenitors
|
| Sample_geo_accession | GSM486405
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | total bone marrow was flushed from freshly dissected femurs and sorted for Lineage negative, c-Kit and Sca1 (LSK) progenitor cells based on c-Myc-eGFP protein expression (Hi and Lo)
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNEasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| | Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486405/suppl/GSM486405.CEL.gz
| | Sample_series_id | GSE19502
| | Sample_data_row_count | 45101
| | |
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GSM486406 | GPL1261 |
|
Myc Hi R2
|
Total RNA, LSK, c-Myc-eGFP Hi
|
strain: WT B6/129
|
WT Lineage negative, c-Kit and Sca1 (LSK) positive c-Myc eGFP Hi expressing bone marrow progenitors
|
| Sample_geo_accession | GSM486406
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | total bone marrow was flushed from freshly dissected femurs and sorted for Lineage negative, c-Kit and Sca1 (LSK) progenitor cells based on c-Myc-eGFP protein expression (Hi and Lo)
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNEasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| | Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486406/suppl/GSM486406.CEL.gz
| | Sample_series_id | GSE19502
| | Sample_data_row_count | 45101
| | |
|
GSM486407 | GPL1261 |
|
Myc Lo R1
|
Total RNA, LSK, c-Myc-eGFP Lo
|
strain: WT B6/129
|
WT Lineage negative, c-Kit and Sca1 (LSK) positive c-Myc eGFP Lo expressing bone marrow progenitors
|
| Sample_geo_accession | GSM486407
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | total bone marrow was flushed from freshly dissected femurs and sorted for Lineage negative, c-Kit and Sca1 (LSK) progenitor cells based on c-Myc-eGFP protein expression (Hi and Lo)
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNEasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| | Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486407/suppl/GSM486407.CEL.gz
| | Sample_series_id | GSE19502
| | Sample_data_row_count | 45101
| | |
|
GSM486408 | GPL1261 |
|
Myc Lo R2
|
Total RNA, LSK, c-Myc-eGFP Lo
|
strain: WT B6/129
|
WT Lineage negative, c-Kit and Sca1 (LSK) positive c-Myc eGFP Lo expressing bone marrow progenitors
|
| Sample_geo_accession | GSM486408
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Dec 15 2009
| | Sample_last_update_date | Dec 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | total bone marrow was flushed from freshly dissected femurs and sorted for Lineage negative, c-Kit and Sca1 (LSK) progenitor cells based on c-Myc-eGFP protein expression (Hi and Lo)
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNEasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| | Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM486nnn/GSM486408/suppl/GSM486408.CEL.gz
| | Sample_series_id | GSE19502
| | Sample_data_row_count | 45101
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