| Sample_geo_accession | GSM488171
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| Sample_status | Public on Dec 09 2010
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| Sample_submission_date | Dec 21 2009
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| Sample_last_update_date | Dec 09 2010
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| Sample_type | RNA
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| Sample_channel_count | 1
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| Sample_organism_ch1 | Homo sapiens
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| Sample_taxid_ch1 | 9606
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| Sample_biomaterial_provider_ch1 | ATCC (American Type Culture Collection)
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| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot. RNA was extracted and quality control was checked before hybridization on Affymetrix microarrays.
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| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
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| Sample_molecule_ch1 | total RNA
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| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
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| Sample_label_ch1 | biotin
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| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
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| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
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| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
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| Sample_data_processing | RMA processing using R (Bioconductor)
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| Sample_platform_id | GPL570
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| Sample_contact_name | Eva,,Bandres
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| Sample_contact_email | ebandres@unav.es
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| Sample_contact_laboratory | Pharmacogenomics lab
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| Sample_contact_department | Cancer Research Program
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| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
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| Sample_contact_address | Avda Pio XII, 55
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| Sample_contact_city | Pamplona
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| Sample_contact_zip/postal_code | 31008
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| Sample_contact_country | Spain
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| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM488nnn/GSM488171/suppl/GSM488171.CEL.gz
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| Sample_series_id | GSE19592
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| Sample_data_row_count | 54675
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