Result

Search results for the GEO ID: GSE19627

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM489337

GPL570

monocytes_+LPS_1

blood, +LPS

cell type: myeloid monocytic cells protocol: 24h medium, 4h LPS stimulation

24h medium, 4h LPS stimulation

Sample_geo_accession	GSM489337
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM oxidized ubiquinone (Q10), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489337/suppl/GSM489337.CEL.gz
Sample_series_id	GSE19625
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489338

GPL570

monocytes_+LPS_2

blood, +LPS

cell type: myeloid monocytic cells protocol: 24h medium, 4h LPS stimulation

24h medium, 4h LPS stimulation

Sample_geo_accession	GSM489338
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM oxidized ubiquinone (Q10), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489338/suppl/GSM489338.CEL.gz
Sample_series_id	GSE19625
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489339

GPL570

monocytes_+LPS_3

blood, +LPS

cell type: myeloid monocytic cells protocol: 24h medium, 4h LPS stimulation

24h medium, 4h LPS stimulation

Sample_geo_accession	GSM489339
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM oxidized ubiquinone (Q10), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489339/suppl/GSM489339.CEL.gz
Sample_series_id	GSE19625
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489343

GPL570

monocytes_-LPS_1

blood, -LPS

cell type: myeloid monocytic cells protocol: 28h medium

28h medium

Sample_geo_accession	GSM489343
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM oxidized ubiquinone (Q10), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489343/suppl/GSM489343.CEL.gz
Sample_series_id	GSE19625
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489344

GPL570

monocytes_-LPS_2

blood, -LPS

cell type: myeloid monocytic cells protocol: 28h medium

28h medium

Sample_geo_accession	GSM489344
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM oxidized ubiquinone (Q10), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489344/suppl/GSM489344.CEL.gz
Sample_series_id	GSE19625
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489345

GPL570

monocytes_-LPS_3

blood, -LPS

cell type: myeloid monocytic cells protocol: 28h medium

28h medium

Sample_geo_accession	GSM489345
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM oxidized ubiquinone (Q10), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489345/suppl/GSM489345.CEL.gz
Sample_series_id	GSE19625
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489352

GPL570

monocytes_Q10H2_1

blood, +Q10H2, +LPS

cell type: myeloid monocytic cells protocol: 24h Q10H2 preincubation, 4h LPS stimulation

24h Q10H2 preincubation, 4h LPS stimulation

Sample_geo_accession	GSM489352
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM ubiquinol (Q10H2), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489352/suppl/GSM489352.CEL.gz
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489353

GPL570

monocytes_Q10H2_2

blood, +Q10H2, +LPS

cell type: myeloid monocytic cells protocol: 24h Q10H2 preincubation, 4h LPS stimulation

24h Q10H2 preincubation, 4h LPS stimulation

Sample_geo_accession	GSM489353
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM ubiquinol (Q10H2), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489353/suppl/GSM489353.CEL.gz
Sample_series_id	GSE19627
Sample_data_row_count	54675

GSM489354

GPL570

monocytes_Q10H2_3

blood, +Q10H2, +LPS

cell type: myeloid monocytic cells protocol: 24h Q10H2 preincubation, 4h LPS stimulation

24h Q10H2 preincubation, 4h LPS stimulation

Sample_geo_accession	GSM489354
Sample_status	Public on Dec 22 2010
Sample_submission_date	Dec 23 2009
Sample_last_update_date	Dec 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	In 6-well plates, cells were pre-incubated with 10 µM ubiquinol (Q10H2), or the respective vehicle controls (KANEKA Corporation, Japan). After 24 hours, cell culture medium was removed and fresh lipopolysaccharide (LPS, E. coli, O55:B5, Sigma Aldrich, Taufkirchen, Germany) containing medium (1 µg/ ml) was added for 4 hours, except in the negative control wells (-LPS).
Sample_growth_protocol_ch1	THP-1 cells were cultivated in RPMI medium 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 10% FCS and 1% antibiotics (penicillin/ streptomycin) in a humidified incubator containing 5% CO2 at 37°C. Twenty four hours before pre-incubation, cells were plated at a density of 3 x 106 per well in a 6-well plate.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were collected in Qiazol-lysis buffer (Qiagen, Hilden, Germany) and stored at -80 °C until isolation of total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (IVT Labeling Kit, Affymetrix).
Sample_hyb_protocol	20 µg of fragmented cRNA was hybridized to a Human Genome U133 Plus 2.0 array for 16 hr at 45 °C at 60 rpm. Arrays were washed on GeneChip® Fluidics station 450 (Affymetrix) and stained with streptavidin-phycoerythrin
Sample_scan_protocol	Microarrays were scanned with a GeneChip® Scanner 3000 7G (Affymetrix) using GCOS software.
Sample_data_processing	Expression data was normalized with GC-RMA algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Frank,,Döring
Sample_contact_email	sek@molprev.uni-kiel.de
Sample_contact_department	Human Nutrition and Food Science
Sample_contact_institute	Christian-Albrechts-University Kiel
Sample_contact_address	Heinrich-Hecht-Platz 10
Sample_contact_city	Kiel
Sample_contact_zip/postal_code	24118
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM489nnn/GSM489354/suppl/GSM489354.CEL.gz
Sample_series_id	GSE19627
Sample_data_row_count	54675

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