Search results for the GEO ID: GSE19639  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM490043 | GPL570 |
|
MCF-7, rep 1
|
MCF-7 cells
|
cell line: MCF-7
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490043
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490043/suppl/GSM490043.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490043/suppl/GSM490043.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490044 | GPL570 |
|
MCF-7, rep 2
|
MCF-7 cells
|
cell line: MCF-7
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490044
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490044/suppl/GSM490044.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490044/suppl/GSM490044.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490045 | GPL570 |
|
MCF-7, rep 3
|
MCF-7 cells
|
cell line: MCF-7
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490045
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490045/suppl/GSM490045.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490045/suppl/GSM490045.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490046 | GPL570 |
|
MCF-7/LTED, rep 1
|
MCF-7/LTED cells
|
cell line: MCF-7
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490046
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490046/suppl/GSM490046.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490046/suppl/GSM490046.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490047 | GPL570 |
|
MCF-7/LTED, rep 2
|
MCF-7/LTED cells
|
cell line: MCF-7
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490047
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490047/suppl/GSM490047.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490047/suppl/GSM490047.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490048 | GPL570 |
|
MCF-7/LTED, rep 3
|
MCF-7/LTED cells
|
cell line: MCF-7
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490048
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490048/suppl/GSM490048.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490048/suppl/GSM490048.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490049 | GPL570 |
|
ZR75-1, rep 1
|
ZR75-1 cells
|
cell line: ZR75-1
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490049
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490049/suppl/GSM490049.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490049/suppl/GSM490049.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490050 | GPL570 |
|
ZR75-1, rep 2
|
ZR75-1 cells
|
cell line: ZR75-1
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490050
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490050/suppl/GSM490050.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490050/suppl/GSM490050.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490051 | GPL570 |
|
ZR75-1, rep 3
|
ZR75-1 cells
|
cell line: ZR75-1
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490051
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490051/suppl/GSM490051.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490051/suppl/GSM490051.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490052 | GPL570 |
|
ZR75-1/LTED, rep 1
|
ZR75-1/LTED cells
|
cell line: ZR75-1
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490052
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490052/suppl/GSM490052.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490052/suppl/GSM490052.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490053 | GPL570 |
|
ZR75-1/LTED, rep 2
|
ZR75-1/LTED cells
|
cell line: ZR75-1
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490053
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490053/suppl/GSM490053.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490053/suppl/GSM490053.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490054 | GPL570 |
|
ZR75-1/LTED, rep 3
|
ZR75-1/LTED cells
|
cell line: ZR75-1
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490054
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490054/suppl/GSM490054.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490054/suppl/GSM490054.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490055 | GPL570 |
|
MDA-361, rep 1
|
MDA-361 cells
|
cell line: MDA-361
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490055
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490055/suppl/GSM490055.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490055/suppl/GSM490055.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490056 | GPL570 |
|
MDA-361, rep 2
|
MDA-361 cells
|
cell line: MDA-361
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490056
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490056/suppl/GSM490056.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490056/suppl/GSM490056.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490057 | GPL570 |
|
MDA-361, rep 3
|
MDA-361 cells
|
cell line: MDA-361
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490057
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490057/suppl/GSM490057.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490057/suppl/GSM490057.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490058 | GPL570 |
|
MDA-361/LTED, rep 1
|
MDA-361/LTED cells
|
cell line: MDA-361
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490058
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490058/suppl/GSM490058.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490058/suppl/GSM490058.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490059 | GPL570 |
|
MDA-361/LTED, rep 2
|
MDA-361/LTED cells
|
cell line: MDA-361
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490059
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490059/suppl/GSM490059.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490059/suppl/GSM490059.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490060 | GPL570 |
|
MDA-361/LTED, rep 3
|
MDA-361/LTED cells
|
cell line: MDA-361
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490060
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490060/suppl/GSM490060.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490060/suppl/GSM490060.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490061 | GPL570 |
|
HCC-1428, rep 1
|
HCC-1428 cells
|
cell line: HCC-1428
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490061
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490061/suppl/GSM490061.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490061/suppl/GSM490061.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490062 | GPL570 |
|
HCC-1428, rep 2
|
HCC-1428 cells
|
cell line: HCC-1428
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490062
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490062/suppl/GSM490062.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490062/suppl/GSM490062.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490063 | GPL570 |
|
HCC-1428, rep 3
|
HCC-1428 cells
|
cell line: HCC-1428
cell type: breast cancer
lted: no
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs.
|
| Sample_geo_accession | GSM490063
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490063/suppl/GSM490063.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490063/suppl/GSM490063.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490064 | GPL570 |
|
HCC-1428/LTED, rep 1
|
HCC-1428/LTED cells
|
cell line: HCC-1428
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490064
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490064/suppl/GSM490064.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490064/suppl/GSM490064.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490065 | GPL570 |
|
HCC-1428/LTED, rep 2
|
HCC-1428/LTED cells
|
cell line: HCC-1428
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490065
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490065/suppl/GSM490065.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490065/suppl/GSM490065.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
|
GSM490066 | GPL570 |
|
HCC-1428/LTED, rep 3
|
HCC-1428/LTED cells
|
cell line: HCC-1428
cell type: breast cancer
lted: yes
|
Gene expression data from cells treated with 10% DCC-FBS x 24 hrs (after long-term culture in same medium).
|
| Sample_geo_accession | GSM490066
| | Sample_status | Public on Jun 15 2010
| | Sample_submission_date | Dec 23 2009
| | Sample_last_update_date | Jun 15 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red). Twenty-four hrs later, RNA was harvested.
| | Sample_growth_protocol_ch1 | Parental cells are maintained in IMEM + 10% FBS + phenol red. LTED cells are maintained in IMEM + 10% DCC-FBS (no phenol red).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
| | Sample_hyb_protocol | 15 ug biotinylated cRNA was fragmented and hybridized to an Affymetrix Genechip Human Genome U133 Plus 2.0 array. Hybridized cRNA was detected using streptavidin coupled to phycoerythrin.
| | Sample_scan_protocol | Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix).
| | Sample_data_processing | Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Todd,W,Miller
| | Sample_contact_email | todd.miller@vanderbilt.edu
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Vanderbilt Univ
| | Sample_contact_address | 2220 Pierce Ave, 777 PRB
| | Sample_contact_city | Nashville
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 37174
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490066/suppl/GSM490066.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM490nnn/GSM490066/suppl/GSM490066.chp.gz
| | Sample_series_id | GSE19639
| | Sample_data_row_count | 54675
| | |
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