Result

Search results for the GEO ID: GSE19672

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM491168

GPL570

48h siRNA rep1

human corporal smooth muscle cells, MaxiK expression silenced for 48h

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491168
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491168/suppl/GSM491168_KD1-HG-U133_Plus_2-3304.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

GSM491169

GPL570

48h siRNA rep2

human corporal smooth muscle cells, MaxiK expression silenced for 48h

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491169
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491169/suppl/GSM491169_KD1-HG-U133_Plus_2-3305.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

GSM491170

GPL570

48h siRNA rep3

human corporal smooth muscle cells, MaxiK expression silenced for 48h

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491170
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491170/suppl/GSM491170_KD1-HG-U133_Plus_2-3519.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

GSM491171

GPL570

48h siRNA rep4

human corporal smooth muscle cells, MaxiK expression silenced for 48h

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491171
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491171/suppl/GSM491171_KD1-HG-U133_Plus_2-3520.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

GSM491172

GPL570

untreated rep1

human corporal smooth muscle cells, untreated

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491172
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491172/suppl/GSM491172_KD1-HG-U133_Plus_2-3306.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

GSM491173

GPL570

untreated rep2

human corporal smooth muscle cells, untreated

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491173
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491173/suppl/GSM491173_KD1-HG-U133_Plus_2-3307.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

GSM491174

GPL570

untreated rep3

human corporal smooth muscle cells, untreated

cell type: corpus cavernosum smooth muscle cell

n/a

Sample_geo_accession	GSM491174
Sample_status	Public on Dec 25 2010
Sample_submission_date	Dec 28 2009
Sample_last_update_date	Dec 25 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Silencing of MaxiK expression was obtained using Stealth Select RNAi (Invitrogen). Cultured cells were transfected with 30nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) following the instructions of the manufacturer.
Sample_growth_protocol_ch1	Penile tissue was obtained from patients undergoing surgery for implantation of prostheses. Explant cell cultures were grown in DMEM, 10% fetal bovine serum (Invitrogen)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested 48h after transfection and RNA was isolated using RNeasy Mini Kit (Quiagen). Efficacy of silencing (>90%) was confirmed by quantitative RT-PCR.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_hyb_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_scan_protocol	Labeling, hybridization and scanning were performed by the Affymetrix facility at Albert Einstein College of Medicine following standard Affymetrix protocols
Sample_data_processing	Gene expression in the silenced cells was compared with controls using AffylmGUI software, from www.bioconductor.org
Sample_platform_id	GPL570
Sample_contact_name	Giulia,,Calenda
Sample_contact_email	giulia.calenda@einstein.yu.edu
Sample_contact_department	Urology
Sample_contact_institute	Albert Einstein College of Medicine
Sample_contact_address	1300 Morris Park Avenue
Sample_contact_city	Bronx
Sample_contact_state	NY
Sample_contact_zip/postal_code	10461
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491174/suppl/GSM491174_KD1-HG-U133_Plus_2-3521.CEL.gz
Sample_series_id	GSE19672
Sample_data_row_count	54675

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