Search results for the GEO ID: GSE19678  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM491345 | GPL570 |
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DACH1-silenced U87TR-Da, DMEMF
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U87MG-derived cell line without DACH1 expression, cultured in serum-containing medium
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cell type: glioblastoma
cell line: U87TR-Da (U87MG-derived)
genome/variation: without DACH1 expression
|
Gene expression data from U87MG-derived cell line without DACH1 expression, cultured in serum-containing medium
|
| Sample_geo_accession | GSM491345
| | Sample_status | Public on Jul 20 2011
| | Sample_submission_date | Dec 28 2009
| | Sample_last_update_date | Jul 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 µg/mL doxycycline for 72 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in 10% FBS-containing DMEM or serum-free Neurobasal medium supplemented with N-2, B-27, bFGF and EGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akira,,Watanabe
| | Sample_contact_email | a.watanabe@cira.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-366-7090
| | Sample_contact_fax | +81-75-366-7090
| | Sample_contact_laboratory | Laboratory of Genome and Epigenome Analysis
| | Sample_contact_department | Center for iPS Cell Research and Application
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Shogoin-Kawahara-cho 53, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491345/suppl/GSM491345.CEL.gz
| | Sample_series_id | GSE19678
| | Sample_data_row_count | 54613
| | |
|
GSM491346 | GPL570 |
|
DACH1-induced U87TR-Da, DMEMF
|
U87MG-derived cell line with DACH1 expression, cultured in serum-containing medium
|
cell type: glioblastoma
cell line: U87TR-Da (U87MG-derived)
genome/variation: with DACH1 expression
|
Gene expression data from U87MG-derived cell line with DACH1 expression, cultured in serum-containing medium
|
| Sample_geo_accession | GSM491346
| | Sample_status | Public on Jul 20 2011
| | Sample_submission_date | Dec 28 2009
| | Sample_last_update_date | Jul 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 µg/mL doxycycline for 72 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in 10% FBS-containing DMEM or serum-free Neurobasal medium supplemented with N-2, B-27, bFGF and EGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akira,,Watanabe
| | Sample_contact_email | a.watanabe@cira.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-366-7090
| | Sample_contact_fax | +81-75-366-7090
| | Sample_contact_laboratory | Laboratory of Genome and Epigenome Analysis
| | Sample_contact_department | Center for iPS Cell Research and Application
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Shogoin-Kawahara-cho 53, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491346/suppl/GSM491346.CEL.gz
| | Sample_series_id | GSE19678
| | Sample_data_row_count | 54613
| | |
|
GSM491347 | GPL570 |
|
DACH1-silenced U87TR-Da, NBE
|
U87MG-derived cell line without DACH1 expression, cultured in serum-free medium
|
cell type: glioblastoma
cell line: U87TR-Da (U87MG-derived)
genome/variation: without DACH1 expression
|
Gene expression data from U87MG-derived cell line without DACH1 expression, cultured in serum-free medium
|
| Sample_geo_accession | GSM491347
| | Sample_status | Public on Jul 20 2011
| | Sample_submission_date | Dec 28 2009
| | Sample_last_update_date | Jul 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 µg/mL doxycycline for 72 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in 10% FBS-containing DMEM or serum-free Neurobasal medium supplemented with N-2, B-27, bFGF and EGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akira,,Watanabe
| | Sample_contact_email | a.watanabe@cira.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-366-7090
| | Sample_contact_fax | +81-75-366-7090
| | Sample_contact_laboratory | Laboratory of Genome and Epigenome Analysis
| | Sample_contact_department | Center for iPS Cell Research and Application
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Shogoin-Kawahara-cho 53, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491347/suppl/GSM491347.CEL.gz
| | Sample_series_id | GSE19678
| | Sample_data_row_count | 54613
| | |
|
GSM491348 | GPL570 |
|
DACH1-induced U87TR-Da, NBE
|
U87MG-derived cell line with DACH1 expression, cultured in serum-free medium
|
cell type: glioblastoma
cell line: U87TR-Da (U87MG-derived)
genome/variation: with DACH1 expression
|
Gene expression data from U87MG-derived cell line with DACH1 expression, cultured in serum-free medium
|
| Sample_geo_accession | GSM491348
| | Sample_status | Public on Jul 20 2011
| | Sample_submission_date | Dec 28 2009
| | Sample_last_update_date | Jul 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 µg/mL doxycycline for 72 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in 10% FBS-containing DMEM or serum-free Neurobasal medium supplemented with N-2, B-27, bFGF and EGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akira,,Watanabe
| | Sample_contact_email | a.watanabe@cira.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-366-7090
| | Sample_contact_fax | +81-75-366-7090
| | Sample_contact_laboratory | Laboratory of Genome and Epigenome Analysis
| | Sample_contact_department | Center for iPS Cell Research and Application
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Shogoin-Kawahara-cho 53, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491348/suppl/GSM491348.CEL.gz
| | Sample_series_id | GSE19678
| | Sample_data_row_count | 54613
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