Search results for the GEO ID: GSE19707  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM491757 | GPL1261 |
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Th2 KO
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Th2 cell from ECM1 Ko mice
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strain: C57BL/6
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gene expression data from liver of ECM1 Ko mice
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| Sample_geo_accession | GSM491757
| | Sample_status | Public on Dec 31 2010
| | Sample_submission_date | Dec 30 2009
| | Sample_last_update_date | Dec 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Spleen of the mice is treated with RBC lysis buffer for 5 minutes, 10μg anti-CD4 antibody is added for each spleen. After incubating for 30 minutes, we washed cell 2 times and add 25ul Goat-anti-rat IgG(miltenyi biotec) for each spleen. Finally, CD4+ cell is separated with MACS separation column, counted and cultured with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 under the Th2 conditions for 4 days (10 ng/mIL-4, 10μg/ml anti-IFN-γ and 10μg/ml anti-IL-12).
| | Sample_growth_protocol_ch1 | Female C57BL6 is grown in SPF condition until 6 weeks, KO mice is confirmed by Realtime RT-PCR and western-blot.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Start with 3μg total RNA and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, One-Cycle Eukaryotic Target Labeling Assay(Lot no. 900493) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.Eukaryotic Target Hybridization,Lot no.900720, is used for the experiment.
| | Sample_scan_protocol | The chips were scanned withthe affymetrix genechip 7G plus and GCOS v1.0 is used for the image processing.
| | Sample_data_processing | genespring 10.0, is used for automatic normalizing the chip data and analysis the fold change.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuan,,Zhang
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
| | Sample_contact_address | Yueyang St. 320#
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491757/suppl/GSM491757.CEL.gz
| | Sample_series_id | GSE19707
| | Sample_data_row_count | 45101
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GSM491758 | GPL1261 |
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Th2 WT
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Th2 cell from WT mice
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strain: C57BL/6
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gene expression data from liver of wildtype mice
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| Sample_geo_accession | GSM491758
| | Sample_status | Public on Dec 31 2010
| | Sample_submission_date | Dec 30 2009
| | Sample_last_update_date | Dec 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Spleen of the mice is treated with RBC lysis buffer for 5 minutes, 10μg anti-CD4 antibody is added for each spleen. After incubating for 30 minutes, we washed cell 2 times and add 25ul Goat-anti-rat IgG(miltenyi biotec) for each spleen. Finally, CD4+ cell is separated with MACS separation column, counted and cultured with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 under the Th2 conditions for 4 days (10 ng/mIL-4, 10μg/ml anti-IFN-γ and 10μg/ml anti-IL-12).
| | Sample_growth_protocol_ch1 | Female C57BL6 is grown in SPF condition until 6 weeks, KO mice is confirmed by Realtime RT-PCR and western-blot.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Start with 3μg total RNA and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, One-Cycle Eukaryotic Target Labeling Assay(Lot no. 900493) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.Eukaryotic Target Hybridization,Lot no.900720, is used for the experiment.
| | Sample_scan_protocol | The chips were scanned withthe affymetrix genechip 7G plus and GCOS v1.0 is used for the image processing.
| | Sample_data_processing | genespring 10.0, is used for automatic normalizing the chip data and analysis the fold change.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuan,,Zhang
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
| | Sample_contact_address | Yueyang St. 320#
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491758/suppl/GSM491758.CEL.gz
| | Sample_series_id | GSE19707
| | Sample_data_row_count | 45101
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