Result

Search results for the GEO ID: GSE19710

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM491929

GPL85

RPaSMC control, microarray 1

Rat pulmonary artery smooth muscle cells (RPaSMC) untreated

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of untreated rat pulmonary artery smooth muscle cells

Sample_geo_accession	GSM491929
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491929/suppl/GSM491929.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491929/suppl/GSM491929.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491929/suppl/GSM491929.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491930

GPL85

RPaSMC exposed to GSNO for 1 hour, microarray1

Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 1 hour

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 1 hour.

Sample_geo_accession	GSM491930
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491930/suppl/GSM491930.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491930/suppl/GSM491930.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491930/suppl/GSM491930.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491931

GPL85

RPaSMC exposed to GSNO for 2 hours, microarray1

Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 2 hours

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 2 hours.

Sample_geo_accession	GSM491931
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491931/suppl/GSM491931.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491931/suppl/GSM491931.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491931/suppl/GSM491931.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491932

GPL85

RPaSMC exposed to GSNO for 4 hours, microarray1

Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 4 hours

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 4 hours.

Sample_geo_accession	GSM491932
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491932/suppl/GSM491932.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491932/suppl/GSM491932.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491932/suppl/GSM491932.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491933

GPL85

RPaSMC control, microarray 2

Rat pulmonary artery smooth muscle cells (RPaSMC) untreated

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of untreated rat pulmonary artery smooth muscle cells

Sample_geo_accession	GSM491933
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491933/suppl/GSM491933.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491933/suppl/GSM491933.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491933/suppl/GSM491933.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491934

GPL85

RPaSMC exposed to GSNO for 1 hour, microarray2

Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 1 hour

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 1 hour.

Sample_geo_accession	GSM491934
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491934/suppl/GSM491934.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491934/suppl/GSM491934.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491934/suppl/GSM491934.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491935

GPL85

RPaSMC exposed to GSNO for 2 hours, microarray2

Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 2 hours

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 2 hours.

Sample_geo_accession	GSM491935
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491935/suppl/GSM491935.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491935/suppl/GSM491935.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491935/suppl/GSM491935.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

GSM491936

GPL85

RPaSMC exposed to GSNO for 4 hours, microarray2

Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 4 hours

strain: Sprague-Dawley tissue: Pulmonary artery smooth muscle cells

Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 4 hours.

Sample_geo_accession	GSM491936
Sample_status	Public on Jun 01 2010
Sample_submission_date	Dec 30 2009
Sample_last_update_date	Dec 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Sample_growth_protocol_ch1	Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
Sample_hyb_protocol	Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Gene Chips were scanned using the GeneChip® Scanner 3000.
Sample_data_processing	Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
Sample_platform_id	GPL85
Sample_contact_name	Andrea,U,Steinbicker
Sample_contact_email	asteinbicker@partners.org
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	55 Fruit Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491936/suppl/GSM491936.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491936/suppl/GSM491936.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM491nnn/GSM491936/suppl/GSM491936.EXP.gz
Sample_series_id	GSE19710
Sample_data_row_count	8799

Make groups for comparisons

(2 groups will be compared at a time)

Select GSMs and click on "Add groups"

Enter the group name here:

Select expression type

Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes