Search results for the GEO ID: GSE19816  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM494826 | GPL570 |
|
HUVECs treated with 10 ng/ml IL1-beta for 4 hours in EGM2 media
|
Human umbilical vein endothelial cells treated with IL1-beta
|
cell type: human umbilical vein endothelial cell
|
Test for HUVEC activation by the inflammatory factor
|
| Sample_geo_accession | GSM494826
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494826/suppl/GSM494826_IL1B_1.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494827 | GPL570 |
|
HUVECs treated with 5 µg/ml Actinomycin D for 4 hours in EGM2 media
|
Human umbilical vein endothelial cells treated with Actinomycin D
|
cell type: human umbilical vein endothelial cell
|
Test for the inhibition of RNA synthesis
|
| Sample_geo_accession | GSM494827
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494827/suppl/GSM494827_ActD_1.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494828 | GPL570 |
|
HUVECs treated with Actinomycin D (5 µg/ml) and IL1-beta (10 ng/ml) in EGM2 media
|
Human umbilical vein endothelial cells treated with IL1-beta and Actinomycin D
|
cell type: human umbilical vein endothelial cell
|
Test that HUVEC activation by the inflammatory factor occurs via de novo RNA synthesis
|
| Sample_geo_accession | GSM494828
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494828/suppl/GSM494828_ActD_IL1B_1.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494829 | GPL570 |
|
HUVECs maintained in EGM2 media, the control rep 1
|
Human umbilical vein endothelial cells
|
cell type: human umbilical vein endothelial cell
|
HUVECs in the basal state
|
| Sample_geo_accession | GSM494829
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494829/suppl/GSM494829_EGM2_1.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494830 | GPL570 |
|
HUVECs maintained in EGM2 media, the control rep 2
|
Human umbilical vein endothelial cells
|
cell type: human umbilical vein endothelial cell
|
HUVECs in the basal state
|
| Sample_geo_accession | GSM494830
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494830/suppl/GSM494830_EGM2_2.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494831 | GPL570 |
|
HUVECs maintained in EGM2 media, the control rep 3
|
Human umbilical vein endothelial cells
|
cell type: human umbilical vein endothelial cell
|
HUVECs in the basal state
|
| Sample_geo_accession | GSM494831
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494831/suppl/GSM494831_EGM2_3.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494832 | GPL570 |
|
HUVECs treated with Hank's buffered salt solution for 4 hours rep 1
|
Human umbilical vein endothelial cells treated with HBSS
|
cell type: human umbilical vein endothelial cell
|
Serum-deprived HUVECs
|
| Sample_geo_accession | GSM494832
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494832/suppl/GSM494832_HBSS_1.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494833 | GPL570 |
|
HUVECs treated with Hank's buffered salt solution for 4 hours rep 2
|
Human umbilical vein endothelial cells treated with HBSS
|
cell type: human umbilical vein endothelial cell
|
Serum-deprived HUVECs
|
| Sample_geo_accession | GSM494833
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494833/suppl/GSM494833_HBSS_2.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494834 | GPL570 |
|
HUVECs treated with Hank's buffered salt solution for 4 hours rep 3
|
Human umbilical vein endothelial cells treated with HBSS
|
cell type: human umbilical vein endothelial cell
|
Serum-deprived HUVECs
|
| Sample_geo_accession | GSM494834
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494834/suppl/GSM494834_HBSS_3.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494835 | GPL570 |
|
HUVECs treated with 4 µg/ml vWF in Hank's buffered salt solution for 4 hours rep 1
|
Human umbilical vein endothelial cells treated with vWF in HBSS
|
cell type: human umbilical vein endothelial cell
|
HUVECs activated with vWF
|
| Sample_geo_accession | GSM494835
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494835/suppl/GSM494835_vWF_1.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
| | |
|
GSM494836 | GPL570 |
|
HUVECs treated with 4 µg/ml vWF in Hank's buffered salt solution for 4 hours rep 2
|
Human umbilical vein endothelial cells treated with vWF in HBSS
|
cell type: human umbilical vein endothelial cell
|
HUVECs activated with vWF
|
| Sample_geo_accession | GSM494836
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494836/suppl/GSM494836_vWF_2.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
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GSM494837 | GPL570 |
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HUVECs treated with 4 µg/ml vWF in Hank's buffered salt solution for 4 hours rep 3
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Human umbilical vein endothelial cells treated with vWF in HBSS
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cell type: human umbilical vein endothelial cell
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HUVECs activated with vWF
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| Sample_geo_accession | GSM494837
| | Sample_status | Public on Nov 10 2010
| | Sample_submission_date | Jan 08 2010
| | Sample_last_update_date | Nov 10 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Confluent HUVECs were treated for 4 hours with Hank's buffered salt solution (HBSS), von Willebrand factor (vWF) in HBSS, IL1-beta in EGM2, Actinomycin D in EGM2 or the combination of IL1-beta with Actinomycin D
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM2 media (Lonza)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hours at 45oC on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 7G Scanner.
| | Sample_data_processing | The data were analyzed using Bioconductor software package
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sergey,V.,Doronin
| | Sample_contact_email | sergey.doronin@stonybrook.edu
| | Sample_contact_department | Physiology&Biophysics
| | Sample_contact_institute | Stony Brook University
| | Sample_contact_address | Nicolls Rd
| | Sample_contact_city | Stony Brook
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11794
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM494nnn/GSM494837/suppl/GSM494837_vWF_3.CEL.gz
| | Sample_series_id | GSE19816
| | Sample_data_row_count | 54675
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