Search results for the GEO ID: GSE19864  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM496089 | GPL570 |
|
MLP-A (Growing)
|
Growing cells expressing a vector control
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing a vector control replicate 1
|
| Sample_geo_accession | GSM496089
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496089/suppl/GSM496089.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496090 | GPL570 |
|
MLP-B (Growing)
|
Growing cells expressing a vector control
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing a vector control replicate 2
|
| Sample_geo_accession | GSM496090
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496090/suppl/GSM496090.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496091 | GPL570 |
|
shRB-A (Growing)
|
Growing cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing shRNA targeting RB replicate 1
|
| Sample_geo_accession | GSM496091
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496091/suppl/GSM496091.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496092 | GPL570 |
|
shRB-B (Growing)
|
Growing cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing shRNA targeting RB replicate 2
|
| Sample_geo_accession | GSM496092
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496092/suppl/GSM496092.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496093 | GPL570 |
|
shp107-A (Growing)
|
Growing cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing shRNA targeting p107 replicate 1
|
| Sample_geo_accession | GSM496093
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496093/suppl/GSM496093.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496094 | GPL570 |
|
shp107-B (Growing)
|
Growing cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing shRNA targeting p107 replicate 2
|
| Sample_geo_accession | GSM496094
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496094/suppl/GSM496094.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496095 | GPL570 |
|
shp130-A (Growing)
|
Growing cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing shRNA targeting p130 replicate 1
|
| Sample_geo_accession | GSM496095
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496095/suppl/GSM496095.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496096 | GPL570 |
|
shp130-B (Growing)
|
Growing cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing shRNA targeting p130 replicate 2
|
| Sample_geo_accession | GSM496096
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496096/suppl/GSM496096.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496097 | GPL570 |
|
MLP-A (0.1% FBS)
|
Quiescent cells expressing a vector control
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing a vector control replicate 1
|
| Sample_geo_accession | GSM496097
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496097/suppl/GSM496097.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496098 | GPL570 |
|
MLP-B (0.1% FBS)
|
Quiescent cells expressing a vector control
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing a vector control replicate 2
|
| Sample_geo_accession | GSM496098
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496098/suppl/GSM496098.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496099 | GPL570 |
|
shRB-A (0.1% FBS)
|
Quiescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing shRNA targeting RB replicate 1
|
| Sample_geo_accession | GSM496099
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496099/suppl/GSM496099.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496100 | GPL570 |
|
shRB-B (0.1% FBS)
|
Quiescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing shRNA targeting RB replicate 2
|
| Sample_geo_accession | GSM496100
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496100/suppl/GSM496100.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496101 | GPL570 |
|
shp107-A (0.1% FBS)
|
Quiescent cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing shRNA targeting p107 replicate 1
|
| Sample_geo_accession | GSM496101
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496101/suppl/GSM496101.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496102 | GPL570 |
|
shp107-B (0.1% FBS)
|
Quiescent cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing shRNA targeting p107 replicate 2
|
| Sample_geo_accession | GSM496102
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496102/suppl/GSM496102.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496103 | GPL570 |
|
shp130-A (0.1% FBS)
|
Quiescent cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing shRNA targeting p130 replicate 1
|
| Sample_geo_accession | GSM496103
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496103/suppl/GSM496103.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496104 | GPL570 |
|
shp130-B (0.1% FBS)
|
Quiescent cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: Low serum quiescence
|
Quiescent cells expressing shRNA targeting p130 replicate 2
|
| Sample_geo_accession | GSM496104
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496104/suppl/GSM496104.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496105 | GPL570 |
|
MLP-A (Confluent)
|
Quiescent cells expressing a vector control
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing a vector control replicate 1
|
| Sample_geo_accession | GSM496105
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496105/suppl/GSM496105.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496106 | GPL570 |
|
MLP-B (Confluent)
|
Quiescent cells expressing a vector control
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing a vector control replicate 2
|
| Sample_geo_accession | GSM496106
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496106/suppl/GSM496106.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496107 | GPL570 |
|
shRB-A (Confluent)
|
Quiescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing shRNA targeting RB replicate 1
|
| Sample_geo_accession | GSM496107
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496107/suppl/GSM496107.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496108 | GPL570 |
|
shRB-B (Confluent)
|
Quiescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing shRNA targeting RB replicate 2
|
| Sample_geo_accession | GSM496108
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496108/suppl/GSM496108.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496109 | GPL570 |
|
shp107-A (Confluent)
|
Quiescent cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing shRNA targeting p107 replicate 1
|
| Sample_geo_accession | GSM496109
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496109/suppl/GSM496109.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496110 | GPL570 |
|
shp107-B (Confluent)
|
Quiescent cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing shRNA targeting p107 replicate 2
|
| Sample_geo_accession | GSM496110
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496110/suppl/GSM496110.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496111 | GPL570 |
|
shp130-A (Confluent)
|
Quiescent cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing shRNA targeting p130 replicate 1
|
| Sample_geo_accession | GSM496111
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496111/suppl/GSM496111.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496112 | GPL570 |
|
shp130-B (Confluent)
|
Quiescent cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: Confluent quiescent
|
Quiescent cells expressing shRNA targeting p130 replicate 2
|
| Sample_geo_accession | GSM496112
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496112/suppl/GSM496112.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496113 | GPL570 |
|
MLP-A (Senescent)
|
Senescent cells expressing a vector control
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing a vector control replicate 1
|
| Sample_geo_accession | GSM496113
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496113/suppl/GSM496113.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496114 | GPL570 |
|
MLP-B (Senescent)
|
Senescent cells expressing a vector control
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing a vector control replicate 2
|
| Sample_geo_accession | GSM496114
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496114/suppl/GSM496114.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496115 | GPL570 |
|
shRB-A (Senescent)
|
Senescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting RB replicate 1
|
| Sample_geo_accession | GSM496115
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496115/suppl/GSM496115.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496116 | GPL570 |
|
shRB-B (Senescent)
|
Senescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting RB replicate 2
|
| Sample_geo_accession | GSM496116
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496116/suppl/GSM496116.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496117 | GPL570 |
|
shp107-A (Senescent)
|
Senescent cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting p107 replicate 1
|
| Sample_geo_accession | GSM496117
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496117/suppl/GSM496117.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496118 | GPL570 |
|
shp107-B (Senescent)
|
Senescent cells expressing shRNA targeting p107
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting p107 replicate 2
|
| Sample_geo_accession | GSM496118
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496118/suppl/GSM496118.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496119 | GPL570 |
|
shp130-A (Senescent)
|
Senescent cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting p130 replicate 1
|
| Sample_geo_accession | GSM496119
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496119/suppl/GSM496119.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496120 | GPL570 |
|
shp130-B (Senescent)
|
Senescent cells expressing shRNA targeting p130
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting p130 replicate 2
|
| Sample_geo_accession | GSM496120
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496120/suppl/GSM496120.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496121 | GPL570 |
|
Growing A E2
|
Growing cells expressing a vector control
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing a vector control replicate 1, experiment 2
|
| Sample_geo_accession | GSM496121
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496121/suppl/GSM496121.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496122 | GPL570 |
|
Growing B E2
|
Growing cells expressing a vector control
|
cell line: IMR90
growth state: Growing
|
Growing cells expressing a vector control replicate 2, experiment 2
|
| Sample_geo_accession | GSM496122
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496122/suppl/GSM496122.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496123 | GPL570 |
|
Senescent A E2
|
Senescent cells expressing a vector control
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing a vector control replicate 1, experiment 2
|
| Sample_geo_accession | GSM496123
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496123/suppl/GSM496123.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496124 | GPL570 |
|
Senescent B E2
|
Senescent cells expressing a vector control
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing a vector control replicate 2, experiment 2
|
| Sample_geo_accession | GSM496124
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496124/suppl/GSM496124.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496125 | GPL570 |
|
shRB-A (senescent) E2
|
Senescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting RB replicate 1, experiment 2
|
| Sample_geo_accession | GSM496125
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496125/suppl/GSM496125.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
|
GSM496126 | GPL570 |
|
shRB-B (Senescent) E2
|
Senescent cells expressing shRNA targeting RB
|
cell line: IMR90
growth state: ras-induced senescence
|
Senescent cells expressing shRNA targeting RB replicate 2, experiment 2
|
| Sample_geo_accession | GSM496126
| | Sample_status | Public on Jan 15 2010
| | Sample_submission_date | Jan 12 2010
| | Sample_last_update_date | Jan 14 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | IMR90 cells were grown in 10% FBS DMEM media unless otherwise stated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNAeasy Mini Kit from Quiegen accordingtheto the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Hybridization was as described in Expression Analysis Technical Manual Affymetrix P/N 702232 Rev 2, (16 hours, 45 degress at 60 rpm), the Wash and Stain Protocol was as described in FS450_0004 using GeneChip Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with the gc-RMA (Robust Multi-Array Averaging with GC content correction) method of BioConductor R package to subtract background normalize intensities and summarize gene expression level.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Agustin,,Chicas
| | Sample_contact_email | agustin_chicas@H3biomedicine.com
| | Sample_contact_institute | Memorial Sloan Kettering
| | Sample_contact_address | One Bungtown Road
| | Sample_contact_city | Cold Spring Harbor
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 11724
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496126/suppl/GSM496126.CEL.gz
| | Sample_series_id | GSE19864
| | Sample_series_id | GSE19899
| | Sample_data_row_count | 54613
| | |
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