Search results for the GEO ID: GSE19875  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM496715 | GPL1261 |
|
A2BAR -/- mouse heart IP120, rep1
|
heart tissue treated with 4 cycles of 5 min ischemia/5 min reperfusion followed by 2 h of reperfusion
|
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496715
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496715/suppl/GSM496715.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496715/suppl/GSM496715.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496716 | GPL1261 |
|
A2BAR -/- mouse heart IP120, rep2
|
heart tissue treated with 4 cycles of 5 min ischemia/5 min reperfusion followed by 2 h of reperfusion
|
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496716
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496716/suppl/GSM496716.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496716/suppl/GSM496716.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496717 | GPL1261 |
|
A2BAR -/- mouse heart IP120, rep3
|
heart tissue treated with 4 cycles of 5 min ischemia/5 min reperfusion followed by 2 h of reperfusion
|
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496717
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496717/suppl/GSM496717.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496717/suppl/GSM496717.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496718 | GPL1261 |
|
A2BAR -/- mouse heart, control, rep1
|
heart tissue, untreated controls
|
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496718
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496718/suppl/GSM496718.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496718/suppl/GSM496718.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496719 | GPL1261 |
|
A2BAR -/- mouse heart, control, rep2
|
heart tissue, untreated controls
|
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496719
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496719/suppl/GSM496719.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496719/suppl/GSM496719.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496720 | GPL1261 |
|
A2BAR -/- mouse heart, control, rep3
|
heart tissue, untreated controls
|
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496720
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496720/suppl/GSM496720.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496720/suppl/GSM496720.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496721 | GPL1261 |
|
BL6/C57 mouse heart IP120, rep1
|
heart tissue treated with 4 cycles of 5 min ischemia/5 min reperfusion followed by 2 h of reperfusion
|
gender: male
strain: BL6/C57
genotype/variation: wildtype
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496721
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496721/suppl/GSM496721.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496721/suppl/GSM496721.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496722 | GPL1261 |
|
BL6/C57 mouse heart IP120, rep2
|
heart tissue treated with 4 cycles of 5 min ischemia/5 min reperfusion followed by 2 h of reperfusion
|
gender: male
strain: BL6/C57
genotype/variation: wildtype
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496722
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496722/suppl/GSM496722.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496722/suppl/GSM496722.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496723 | GPL1261 |
|
BL6/C57 mouse heart IP120, rep3
|
heart tissue treated with 4 cycles of 5 min ischemia/5 min reperfusion followed by 2 h of reperfusion
|
gender: male
strain: BL6/C57
genotype/variation: wildtype
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496723
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496723/suppl/GSM496723.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496723/suppl/GSM496723.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496724 | GPL1261 |
|
BL6/C57 mouse heart, control, rep1
|
heart tissue, untreated controls
|
gender: male
strain: BL6/C57
genotype/variation: wildtype
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496724
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496724/suppl/GSM496724.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496724/suppl/GSM496724.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496725 | GPL1261 |
|
BL6/C57 mouse heart, control, rep2
|
heart tissue, untreated controls
|
gender: male
strain: BL6/C57
genotype/variation: wildtype
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496725
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496725/suppl/GSM496725.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496725/suppl/GSM496725.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
GSM496726 | GPL1261 |
|
BL6/C57 mouse heart, control, rep3
|
heart tissue, untreated controls
|
gender: male
strain: BL6/C57
genotype/variation: wildtype
age: 12 weeks
|
snap frozen, left ventricle, area at risk
|
| Sample_geo_accession | GSM496726
| | Sample_status | Public on Jan 14 2010
| | Sample_submission_date | Jan 13 2010
| | Sample_last_update_date | Jan 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
| | Sample_growth_protocol_ch1 | male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tobias,,Eckle
| | Sample_contact_email | tobias.eckle@ucdenver.edu
| | Sample_contact_phone | 72-949-5646
| | Sample_contact_fax | 303-724-2852
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 E 19th Avenue
| | Sample_contact_city | Denver
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.ucdenver.edu/academics/colleges/medicalschool/programs/CSD/People/Faculty/Pages/EckleTobias.aspx
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496726/suppl/GSM496726.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM496nnn/GSM496726/suppl/GSM496726.CHP.gz
| | Sample_series_id | GSE19875
| | Sample_data_row_count | 45101
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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