Result

Search results for the GEO ID: GSE19938

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM498222

GPL1261

mpkCCD clone 11 exposed to vehicle for 5 days, biological replicate 1

mpkCCD clone 11 cells

cell type: mouse kidney cortical collecting duct cells cell line: mpkCCD clone 11

vasopressin-mediated endogenous aquaporin 2 expression Gene expression data from cells in response to vehicle exposure for 5 days

Sample_geo_accession	GSM498222
Sample_status	Public on Dec 09 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Dec 09 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Once transepithelial resistance reached 5kohm per centimeter square and higher, the cells were exposed to the vasopressin V2 receptor analog, dDAVP, at a physiological concentration, 0.1nM, for 5 days. Control experiments were done with cells exposed to vehicle alone.
Sample_growth_protocol_ch1	The mpkCCD cells were grown on membrane supports to permit polarization.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
Sample_hyb_protocol	Labeled cRNA were hybridized to Mouse Genome 430 2.0 Arrays in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
Sample_scan_protocol	Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
Sample_data_processing	Transcript intensity values were obtained with Affymetrix GeneChip Operating System software version 1.4. Detected signals were normalized based on MAS5 and RMA algorithms using Affymetrix Expression Console software version 1.1. Probe sets that had absent calls in all 6 arrays were eliminated from further analysis. Statistical analysis was based on RMA normalized signal intensity values.
Sample_platform_id	GPL1261
Sample_contact_name	Ming-Jiun,,Yu
Sample_contact_email	mjyu@ntu.edu.tw
Sample_contact_laboratory	Systems Biology of Epithelia Laboratory
Sample_contact_department	Institute of Biochemistry and Molecular Biology
Sample_contact_institute	National Taiwan University College of Medicine
Sample_contact_address	Rm 816, No. 1 Ren-Ai Road Section 1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_contact_web_link	http://sbel.mc.ntu.edu.tw
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498222/suppl/GSM498222_PLX071220_clone11_vehicle1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498222/suppl/GSM498222_PLX071220_clone11_vehicle1.CHP.gz
Sample_series_id	GSE19938
Sample_data_row_count	28287

GSM498223

GPL1261

mpkCCD clone 11 exposed to vehicle for 5 days, biological replicate 2

mpkCCD clone 11 cells

cell type: mouse kidney cortical collecting duct cells cell line: mpkCCD clone 11

vasopressin-mediated endogenous aquaporin 2 expression Gene expression data from cells in response to vehicle exposure for 5 days

Sample_geo_accession	GSM498223
Sample_status	Public on Dec 09 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Dec 09 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Once transepithelial resistance reached 5kohm per centimeter square and higher, the cells were exposed to the vasopressin V2 receptor analog, dDAVP, at a physiological concentration, 0.1nM, for 5 days. Control experiments were done with cells exposed to vehicle alone.
Sample_growth_protocol_ch1	The mpkCCD cells were grown on membrane supports to permit polarization.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
Sample_hyb_protocol	Labeled cRNA were hybridized to Mouse Genome 430 2.0 Arrays in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
Sample_scan_protocol	Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
Sample_data_processing	Transcript intensity values were obtained with Affymetrix GeneChip Operating System software version 1.4. Detected signals were normalized based on MAS5 and RMA algorithms using Affymetrix Expression Console software version 1.1. Probe sets that had absent calls in all 6 arrays were eliminated from further analysis. Statistical analysis was based on RMA normalized signal intensity values.
Sample_platform_id	GPL1261
Sample_contact_name	Ming-Jiun,,Yu
Sample_contact_email	mjyu@ntu.edu.tw
Sample_contact_laboratory	Systems Biology of Epithelia Laboratory
Sample_contact_department	Institute of Biochemistry and Molecular Biology
Sample_contact_institute	National Taiwan University College of Medicine
Sample_contact_address	Rm 816, No. 1 Ren-Ai Road Section 1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_contact_web_link	http://sbel.mc.ntu.edu.tw
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498223/suppl/GSM498223_PLX071220_clone11_vehicle2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498223/suppl/GSM498223_PLX071220_clone11_vehicle2.CHP.gz
Sample_series_id	GSE19938
Sample_data_row_count	28287

GSM498224

GPL1261

mpkCCD clone 11 exposed to vehicle for 5 days, biological replicate 3

mpkCCD clone 11 cells

cell type: mouse kidney cortical collecting duct cells cell line: mpkCCD clone 11

vasopressin-mediated endogenous aquaporin 2 expression Gene expression data from cells in response to vehicle exposure for 5 days

Sample_geo_accession	GSM498224
Sample_status	Public on Dec 09 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Dec 09 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Once transepithelial resistance reached 5kohm per centimeter square and higher, the cells were exposed to the vasopressin V2 receptor analog, dDAVP, at a physiological concentration, 0.1nM, for 5 days. Control experiments were done with cells exposed to vehicle alone.
Sample_growth_protocol_ch1	The mpkCCD cells were grown on membrane supports to permit polarization.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
Sample_hyb_protocol	Labeled cRNA were hybridized to Mouse Genome 430 2.0 Arrays in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
Sample_scan_protocol	Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
Sample_data_processing	Transcript intensity values were obtained with Affymetrix GeneChip Operating System software version 1.4. Detected signals were normalized based on MAS5 and RMA algorithms using Affymetrix Expression Console software version 1.1. Probe sets that had absent calls in all 6 arrays were eliminated from further analysis. Statistical analysis was based on RMA normalized signal intensity values.
Sample_platform_id	GPL1261
Sample_contact_name	Ming-Jiun,,Yu
Sample_contact_email	mjyu@ntu.edu.tw
Sample_contact_laboratory	Systems Biology of Epithelia Laboratory
Sample_contact_department	Institute of Biochemistry and Molecular Biology
Sample_contact_institute	National Taiwan University College of Medicine
Sample_contact_address	Rm 816, No. 1 Ren-Ai Road Section 1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_contact_web_link	http://sbel.mc.ntu.edu.tw
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498224/suppl/GSM498224_PLX071220_clone11_vehicle3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498224/suppl/GSM498224_PLX071220_clone11_vehicle3.CHP.gz
Sample_series_id	GSE19938
Sample_data_row_count	28287

GSM498225

GPL1261

mpkCCD clone 11 exposed to dDAVP for 5 days, biological replicate 1

mpkCCD clone 11 cells

cell type: mouse kidney cortical collecting duct cells cell line: mpkCCD clone 11

vasopressin-mediated endogenous aquaporin 2 expression Gene expression data from cells in response to 0.1nM dDAVP exposure for 5 days

Sample_geo_accession	GSM498225
Sample_status	Public on Dec 09 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Dec 09 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Once transepithelial resistance reached 5kohm per centimeter square and higher, the cells were exposed to the vasopressin V2 receptor analog, dDAVP, at a physiological concentration, 0.1nM, for 5 days. Control experiments were done with cells exposed to vehicle alone.
Sample_growth_protocol_ch1	The mpkCCD cells were grown on membrane supports to permit polarization.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
Sample_hyb_protocol	Labeled cRNA were hybridized to Mouse Genome 430 2.0 Arrays in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
Sample_scan_protocol	Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
Sample_data_processing	Transcript intensity values were obtained with Affymetrix GeneChip Operating System software version 1.4. Detected signals were normalized based on MAS5 and RMA algorithms using Affymetrix Expression Console software version 1.1. Probe sets that had absent calls in all 6 arrays were eliminated from further analysis. Statistical analysis was based on RMA normalized signal intensity values.
Sample_platform_id	GPL1261
Sample_contact_name	Ming-Jiun,,Yu
Sample_contact_email	mjyu@ntu.edu.tw
Sample_contact_laboratory	Systems Biology of Epithelia Laboratory
Sample_contact_department	Institute of Biochemistry and Molecular Biology
Sample_contact_institute	National Taiwan University College of Medicine
Sample_contact_address	Rm 816, No. 1 Ren-Ai Road Section 1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_contact_web_link	http://sbel.mc.ntu.edu.tw
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498225/suppl/GSM498225_PLX071220_clone11_dDAVP1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498225/suppl/GSM498225_PLX071220_clone11_dDAVP1.CHP.gz
Sample_series_id	GSE19938
Sample_data_row_count	28287

GSM498226

GPL1261

mpkCCD clone 11 exposed to dDAVP for 5 days, biological replicate 2

mpkCCD clone 11 cells

cell type: mouse kidney cortical collecting duct cells cell line: mpkCCD clone 11

vasopressin-mediated endogenous aquaporin 2 expression Gene expression data from cells in response to 0.1nM dDAVP exposure for 5 days

Sample_geo_accession	GSM498226
Sample_status	Public on Dec 09 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Dec 09 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Once transepithelial resistance reached 5kohm per centimeter square and higher, the cells were exposed to the vasopressin V2 receptor analog, dDAVP, at a physiological concentration, 0.1nM, for 5 days. Control experiments were done with cells exposed to vehicle alone.
Sample_growth_protocol_ch1	The mpkCCD cells were grown on membrane supports to permit polarization.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
Sample_hyb_protocol	Labeled cRNA were hybridized to Mouse Genome 430 2.0 Arrays in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
Sample_scan_protocol	Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
Sample_data_processing	Transcript intensity values were obtained with Affymetrix GeneChip Operating System software version 1.4. Detected signals were normalized based on MAS5 and RMA algorithms using Affymetrix Expression Console software version 1.1. Probe sets that had absent calls in all 6 arrays were eliminated from further analysis. Statistical analysis was based on RMA normalized signal intensity values.
Sample_platform_id	GPL1261
Sample_contact_name	Ming-Jiun,,Yu
Sample_contact_email	mjyu@ntu.edu.tw
Sample_contact_laboratory	Systems Biology of Epithelia Laboratory
Sample_contact_department	Institute of Biochemistry and Molecular Biology
Sample_contact_institute	National Taiwan University College of Medicine
Sample_contact_address	Rm 816, No. 1 Ren-Ai Road Section 1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_contact_web_link	http://sbel.mc.ntu.edu.tw
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498226/suppl/GSM498226_PLX071220_clone11_dDAVP2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498226/suppl/GSM498226_PLX071220_clone11_dDAVP2.CHP.gz
Sample_series_id	GSE19938
Sample_data_row_count	28287

GSM498227

GPL1261

mpkCCD clone 11 exposed to dDAVP for 5 days, biological replicate 3

mpkCCD clone 11 cells

cell type: mouse kidney cortical collecting duct cells cell line: mpkCCD clone 11

vasopressin-mediated endogenous aquaporin 2 expression Gene expression data from cells in response to 0.1nM dDAVP exposure for 5 days

Sample_geo_accession	GSM498227
Sample_status	Public on Dec 09 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Dec 09 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Once transepithelial resistance reached 5kohm per centimeter square and higher, the cells were exposed to the vasopressin V2 receptor analog, dDAVP, at a physiological concentration, 0.1nM, for 5 days. Control experiments were done with cells exposed to vehicle alone.
Sample_growth_protocol_ch1	The mpkCCD cells were grown on membrane supports to permit polarization.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
Sample_hyb_protocol	Labeled cRNA were hybridized to Mouse Genome 430 2.0 Arrays in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
Sample_scan_protocol	Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
Sample_data_processing	Transcript intensity values were obtained with Affymetrix GeneChip Operating System software version 1.4. Detected signals were normalized based on MAS5 and RMA algorithms using Affymetrix Expression Console software version 1.1. Probe sets that had absent calls in all 6 arrays were eliminated from further analysis. Statistical analysis was based on RMA normalized signal intensity values.
Sample_platform_id	GPL1261
Sample_contact_name	Ming-Jiun,,Yu
Sample_contact_email	mjyu@ntu.edu.tw
Sample_contact_laboratory	Systems Biology of Epithelia Laboratory
Sample_contact_department	Institute of Biochemistry and Molecular Biology
Sample_contact_institute	National Taiwan University College of Medicine
Sample_contact_address	Rm 816, No. 1 Ren-Ai Road Section 1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_contact_web_link	http://sbel.mc.ntu.edu.tw
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498227/suppl/GSM498227_PLX071220_clone11_dDAVP3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498227/suppl/GSM498227_PLX071220_clone11_dDAVP3.CHP.gz
Sample_series_id	GSE19938
Sample_data_row_count	28287

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