Result

Search results for the GEO ID: GSE19954

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM498649

GPL1261

Control-1 (chow-fed C57BL/6 mouse)

epididymal fat tissue, control chow

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: normal chow treatment: none

Chow-fed C57BL/6 mouse.

Sample_geo_accession	GSM498649
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498649/suppl/GSM498649.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498649/suppl/GSM498649.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498650

GPL1261

Control-2 (chow-fed C57BL/6 mouse)

epididymal fat tissue, control chow

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: normal chow treatment: none

Chow-fed C57BL/6 mouse.

Sample_geo_accession	GSM498650
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498650/suppl/GSM498650.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498650/suppl/GSM498650.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498651

GPL1261

HFD-1 (high fat-fed C57BL/6 mouse)

epididymal fat tissue, high-fat diet

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: high fat treatment: none

High fat-fed C57BL/6 mouse.

Sample_geo_accession	GSM498651
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498651/suppl/GSM498651.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498651/suppl/GSM498651.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498652

GPL1261

HFD-2 (high fat-fed C57BL/6 mouse)

epididymal fat tissue, high-fat diet

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: high fat treatment: none

High fat-fed C57BL/6 mouse.

Sample_geo_accession	GSM498652
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498652/suppl/GSM498652.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498652/suppl/GSM498652.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498653

GPL1261

HFD+Tel-1 (high fat-fed C57BL/6 mouse treated with telmisartan)

epididymal fat tissue, high-fat diet, telmisartan

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: high fat treatment: telmisartan

High fat-fed C57BL/6 mouse treated with telmisartan.

Sample_geo_accession	GSM498653
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498653/suppl/GSM498653.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498653/suppl/GSM498653.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498654

GPL1261

HFD+Tel-2 (high fat-fed C57BL/6 mouse treated with telmisartan)

epididymal fat tissue, high-fat diet, telmisartan

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: high fat treatment: telmisartan

High fat-fed C57BL/6 mouse treated with telmisartan.

Sample_geo_accession	GSM498654
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498654/suppl/GSM498654.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498654/suppl/GSM498654.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498655

GPL1261

HFD+Can-1 (high fat-fed C57BL/6 mouse treated with candesartan)

epididymal fat tissue, high-fat diet, candesartan

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: high fat treatment: candesartan

High fat-fed C57BL/6 mouse treated with candesartan.

Sample_geo_accession	GSM498655
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498655/suppl/GSM498655.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498655/suppl/GSM498655.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

GSM498656

GPL1261

HFD+Can-2 (high fat-fed C57BL/6 mouse treated with candesartan)

epididymal fat tissue, high-fat diet, candesartan

strain: C57BL/6J gender: male tissue: epididymal fat tissue diet: high fat treatment: candesartan

High fat-fed C57BL/6 mouse treated with candesartan.

Sample_geo_accession	GSM498656
Sample_status	Public on Jan 23 2010
Sample_submission_date	Jan 19 2010
Sample_last_update_date	Jan 22 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Six-week-old male C57BL/6J mice were purchased from CLEA Japan. The mice were fed a chow that contained 10% of its calories from fat (control) or a high-fat diet (HFD) that contained 30% of its calories from fat for 24 weeks.　The high fat-fed mice were randomized to 3 groups. Either telmisartan (~3 mg/kg/day) in drinking water (HFD+Tel), candesartan (~3 mg/kg/day) in drinking water (HFD+Can), or a HFD without any drugs (HFD) was administered for the next 5 weeks. Epididymal adipose tissues were rapidly removed from each mouse.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the epididymal fat tissue using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL1261
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498656/suppl/GSM498656.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM498nnn/GSM498656/suppl/GSM498656.CHP.gz
Sample_series_id	GSE19954
Sample_data_row_count	45101

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