Result

Search results for the GEO ID: GSE19979

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM499307

GPL1261

uroplakin1-ve

postnatal day 7 bladder epithelial DsRed -ve cells

gene reported: B6;129P-Upk1btm1Zhang/J strain: FVB/N x C57BL/6 Sex: Male developmental stage: P7 theiler stage: TS28 somite count: n/a developmental landmark: n/a cell type: bladder epithelial cell

>> Amplification protocol << Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies) Target Amplified protocol: Potter Protocols Rounds of amplification: 2 URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:13407

Sample_geo_accession	GSM499307
Sample_status	Public on Jan 22 2010
Sample_submission_date	Jan 21 2010
Sample_last_update_date	Dec 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: n/a
Sample_treatment_protocol_ch1	Pooled sample: no
Sample_treatment_protocol_ch1	Dissection Method: Bladder microdissected into rings and treated with 1 mg/ml trypsin in Tyrode's solution to dissociate the epithelium as a layer. This was then further digested with Blendzymes 1 & 4.
Sample_treatment_protocol_ch1	At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in Tyrode’s solution for 30 minutes at 37 °C. The layers were separated by gentle micro-dissection and the epithelial layer was further digested in a mixture of Blendzymes 1 & 4 at 37 °C until a single cell suspension was obtained. The samples were placed in RLT and stored at -80 °C. RNA was prepared and the Epicentre 2-round amplification scheme described on the GUDMAP web site was performed. Affymetrix GeneChip Mouse Genome 430 2.0 Array chips were used to interrogate the amplified RNA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen:RNEASY
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 10ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM499nnn/GSM499307/suppl/GSM499307_Upl_1_ve_091008_MOE430_2.CEL.gz
Sample_series_id	GSE19979
Sample_data_row_count	45101

GSM499308

GPL1261

uroplakin2-ve

postnatal day 7 bladder epithelial DsRed -ve cells

gene reported: B6;129P-Upk1btm1Zhang/J strain: FVB/N x C57BL/6 Sex: Male developmental stage: P7 theiler stage: TS28 somite count: n/a developmental landmark: n/a cell type: bladder epithelial cell

Sample_geo_accession	GSM499308
Sample_status	Public on Jan 22 2010
Sample_submission_date	Jan 21 2010
Sample_last_update_date	Dec 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: n/a
Sample_treatment_protocol_ch1	Pooled sample: no
Sample_treatment_protocol_ch1	Dissection Method: Bladder microdissected into rings and treated with 1 mg/ml trypsin in Tyrode's solution to dissociate the epithelium as a layer. This was then further digested with Blendzymes 1 & 4.
Sample_treatment_protocol_ch1	At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in Tyrode’s solution for 30 minutes at 37 °C. The layers were separated by gentle micro-dissection and the epithelial layer was further digested in a mixture of Blendzymes 1 & 4 at 37 °C until a single cell suspension was obtained. The samples were placed in RLT and stored at -80 °C. RNA was prepared and the Epicentre 2-round amplification scheme described on the GUDMAP web site was performed. Affymetrix GeneChip Mouse Genome 430 2.0 Array chips were used to interrogate the amplified RNA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen:RNEASY
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 10ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM499nnn/GSM499308/suppl/GSM499308_Upl_2_ve_091008_MOE430_2.CEL.gz
Sample_series_id	GSE19979
Sample_data_row_count	45101

GSM499309

GPL1261

uroplakin5-ve

postnatal day 7 bladder epithelial DsRed -ve cells

gene reported: B6;129P-Upk1btm1Zhang/J strain: FVB/N x C57BL/6 Sex: Male developmental stage: P7 theiler stage: TS28 somite count: n/a developmental landmark: n/a cell type: bladder epithelial cell

Sample_geo_accession	GSM499309
Sample_status	Public on Jan 22 2010
Sample_submission_date	Jan 21 2010
Sample_last_update_date	Dec 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: n/a
Sample_treatment_protocol_ch1	Pooled sample: no
Sample_treatment_protocol_ch1	Dissection Method: Bladder microdissected into rings and treated with 1 mg/ml trypsin in Tyrode's solution to dissociate the epithelium as a layer. This was then further digested with Blendzymes 1 & 4.
Sample_treatment_protocol_ch1	At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in Tyrode’s solution for 30 minutes at 37 °C. The layers were separated by gentle micro-dissection and the epithelial layer was further digested in a mixture of Blendzymes 1 & 4 at 37 °C until a single cell suspension was obtained. The samples were placed in RLT and stored at -80 °C. RNA was prepared and the Epicentre 2-round amplification scheme described on the GUDMAP web site was performed. Affymetrix GeneChip Mouse Genome 430 2.0 Array chips were used to interrogate the amplified RNA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen:RNEASY
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 10ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM499nnn/GSM499309/suppl/GSM499309_Upl_5_ve_091008_MOE430_2.CEL.gz
Sample_series_id	GSE19979
Sample_data_row_count	45101

GSM499310

GPL1261

uroplakin1+ve

postnatal day 7 bladder epithelial DsRed +ve cells

gene reported: B6;129P-Upk1btm1Zhang/J strain: FVB/N x C57BL/6 Sex: Male developmental stage: P7 theiler stage: TS28 somite count: n/a developmental landmark: n/a cell type: bladder epithelial cell

Sample_geo_accession	GSM499310
Sample_status	Public on Jan 22 2010
Sample_submission_date	Jan 21 2010
Sample_last_update_date	Dec 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: n/a
Sample_treatment_protocol_ch1	Pooled sample: no
Sample_treatment_protocol_ch1	Dissection Method: Bladder microdissected into rings and treated with 1 mg/ml trypsin in Tyrode's solution to dissociate the epithelium as a layer. This was then further digested with Blendzymes 1 & 4.
Sample_treatment_protocol_ch1	At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in Tyrode’s solution for 30 minutes at 37 °C. The layers were separated by gentle micro-dissection and the epithelial layer was further digested in a mixture of Blendzymes 1 & 4 at 37 °C until a single cell suspension was obtained. The samples were placed in RLT and stored at -80 °C. RNA was prepared and the Epicentre 2-round amplification scheme described on the GUDMAP web site was performed. Affymetrix GeneChip Mouse Genome 430 2.0 Array chips were used to interrogate the amplified RNA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen:RNEASY
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 10ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM499nnn/GSM499310/suppl/GSM499310_Upl_1_ve_091008_MOE430_2.CEL.gz
Sample_series_id	GSE19979
Sample_data_row_count	45101

GSM499311

GPL1261

uroplakin2+ve

postnatal day 7 bladder epithelial DsRed +ve cells

gene reported: B6;129P-Upk1btm1Zhang/J strain: FVB/N x C57BL/6 Sex: Male developmental stage: P7 theiler stage: TS28 somite count: n/a developmental landmark: n/a cell type: bladder epithelial cell

Sample_geo_accession	GSM499311
Sample_status	Public on Jan 22 2010
Sample_submission_date	Jan 21 2010
Sample_last_update_date	Dec 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: n/a
Sample_treatment_protocol_ch1	Pooled sample: no
Sample_treatment_protocol_ch1	Dissection Method: Bladder microdissected into rings and treated with 1 mg/ml trypsin in Tyrode's solution to dissociate the epithelium as a layer. This was then further digested with Blendzymes 1 & 4.
Sample_treatment_protocol_ch1	At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in Tyrode’s solution for 30 minutes at 37 °C. The layers were separated by gentle micro-dissection and the epithelial layer was further digested in a mixture of Blendzymes 1 & 4 at 37 °C until a single cell suspension was obtained. The samples were placed in RLT and stored at -80 °C. RNA was prepared and the Epicentre 2-round amplification scheme described on the GUDMAP web site was performed. Affymetrix GeneChip Mouse Genome 430 2.0 Array chips were used to interrogate the amplified RNA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen:RNEASY
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 10ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM499nnn/GSM499311/suppl/GSM499311_Upl_2_ve_091008_MOE430_2.CEL.gz
Sample_series_id	GSE19979
Sample_data_row_count	45101

GSM499312

GPL1261

uroplakin5+ve

postnatal day 7 bladder epithelial DsRed +ve cells

gene reported: B6;129P-Upk1btm1Zhang/J strain: FVB/N x C57BL/6 Sex: Male developmental stage: P7 theiler stage: TS28 somite count: n/a developmental landmark: n/a cell type: bladder epithelial cell

Sample_geo_accession	GSM499312
Sample_status	Public on Jan 22 2010
Sample_submission_date	Jan 21 2010
Sample_last_update_date	Dec 26 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: n/a
Sample_treatment_protocol_ch1	Pooled sample: no
Sample_treatment_protocol_ch1	Dissection Method: Bladder microdissected into rings and treated with 1 mg/ml trypsin in Tyrode's solution to dissociate the epithelium as a layer. This was then further digested with Blendzymes 1 & 4.
Sample_treatment_protocol_ch1	At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in Tyrode’s solution for 30 minutes at 37 °C. The layers were separated by gentle micro-dissection and the epithelial layer was further digested in a mixture of Blendzymes 1 & 4 at 37 °C until a single cell suspension was obtained. The samples were placed in RLT and stored at -80 °C. RNA was prepared and the Epicentre 2-round amplification scheme described on the GUDMAP web site was performed. Affymetrix GeneChip Mouse Genome 430 2.0 Array chips were used to interrogate the amplified RNA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen:RNEASY
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 10ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM499nnn/GSM499312/suppl/GSM499312_Upl_5_ve_091008_MOE430_2.CEL.gz
Sample_series_id	GSE19979
Sample_data_row_count	45101

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