Search results for the GEO ID: GSE20054  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM501363 | GPL1261 |
|
Ezh2-Cre1
|
immortalized mouse brown preadipocyte
|
cell type: brown preadipocyte
genotype: Ezh2 -/-
|
Gene expression data from Ezh2-/- brwon preadipocytes
|
| Sample_geo_accession | GSM501363
| | Sample_status | Public on Mar 19 2010
| | Sample_submission_date | Jan 26 2010
| | Sample_last_update_date | Mar 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | All cells were routinely cultured in DMEM plus 10% FBS (Hyclone). For selection, count cells and plate 5x105/10cm, add 150ug/ml Hygromycin for 2~3days. Repeat the selection one more time.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lifeng,,Wang
| | Sample_contact_email | wanglifeng@niddk.nih.gov
| | Sample_contact_phone | 301-451-1807
| | Sample_contact_laboratory | CEB
| | Sample_contact_department | NIDDK
| | Sample_contact_institute | NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM501nnn/GSM501363/suppl/GSM501363_12-04-09_KGeEzh2Cre1_Mouse430_2_Ezh2_Cre1.CEL.gz
| | Sample_series_id | GSE20054
| | Sample_data_row_count | 45101
| | |
|
GSM501364 | GPL1261 |
|
Ezh2-Cre2
|
immortalized mouse brown preadipocyte
|
cell type: brown preadipocyte
genotype: Ezh2 -/-
|
Gene expression data from Ezh2-/- brwon preadipocytes
|
| Sample_geo_accession | GSM501364
| | Sample_status | Public on Mar 19 2010
| | Sample_submission_date | Jan 26 2010
| | Sample_last_update_date | Mar 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | All cells were routinely cultured in DMEM plus 10% FBS (Hyclone). For selection, count cells and plate 5x105/10cm, add 150ug/ml Hygromycin for 2~3days. Repeat the selection one more time.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lifeng,,Wang
| | Sample_contact_email | wanglifeng@niddk.nih.gov
| | Sample_contact_phone | 301-451-1807
| | Sample_contact_laboratory | CEB
| | Sample_contact_department | NIDDK
| | Sample_contact_institute | NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM501nnn/GSM501364/suppl/GSM501364_12-04-09_KGeEzh2Cre2_Mouse430_2_Ezh2_Cre2.CEL.gz
| | Sample_series_id | GSE20054
| | Sample_data_row_count | 45101
| | |
|
GSM501365 | GPL1261 |
|
Ezh2-Vec1
|
immortalized mouse brown preadipocyte
|
cell type: brown preadipocyte
genotype: Ezh2 +/+
|
Gene expression data from Ezh2+/+ brwon preadipocytes
|
| Sample_geo_accession | GSM501365
| | Sample_status | Public on Mar 19 2010
| | Sample_submission_date | Jan 26 2010
| | Sample_last_update_date | Mar 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | All cells were routinely cultured in DMEM plus 10% FBS (Hyclone). For selection, count cells and plate 5x105/10cm, add 150ug/ml Hygromycin for 2~3days. Repeat the selection one more time.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lifeng,,Wang
| | Sample_contact_email | wanglifeng@niddk.nih.gov
| | Sample_contact_phone | 301-451-1807
| | Sample_contact_laboratory | CEB
| | Sample_contact_department | NIDDK
| | Sample_contact_institute | NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM501nnn/GSM501365/suppl/GSM501365_12-04-09_KGeEzh2Vec1_Mouse430_2_Ezh2_Vec1.CEL.gz
| | Sample_series_id | GSE20054
| | Sample_data_row_count | 45101
| | |
|
GSM501366 | GPL1261 |
|
Ezh2-Vec2
|
immortalized mouse brown preadipocyte
|
cell type: brown preadipocyte
genotype: Ezh2 +/+
|
Gene expression data from Ezh2+/+ brwon preadipocytes
|
| Sample_geo_accession | GSM501366
| | Sample_status | Public on Mar 19 2010
| | Sample_submission_date | Jan 26 2010
| | Sample_last_update_date | Mar 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | All cells were routinely cultured in DMEM plus 10% FBS (Hyclone). For selection, count cells and plate 5x105/10cm, add 150ug/ml Hygromycin for 2~3days. Repeat the selection one more time.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lifeng,,Wang
| | Sample_contact_email | wanglifeng@niddk.nih.gov
| | Sample_contact_phone | 301-451-1807
| | Sample_contact_laboratory | CEB
| | Sample_contact_department | NIDDK
| | Sample_contact_institute | NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM501nnn/GSM501366/suppl/GSM501366_12-04-09_KGeEzh2Vec2_Mouse430_2_Ezh2_Vec2.CEL.gz
| | Sample_series_id | GSE20054
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
| Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
| Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
| |
Filter results by number of probes |
|
|
