Search results for the GEO ID: GSE20149  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM505315 | GPL85 |
|
Normal AM rep1
|
Alveolar macrophages from normal rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from normal rats
|
| Sample_geo_accession | GSM505315
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505315/suppl/GSM505315_CL01R001.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505315/suppl/GSM505315_CL01R001.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505316 | GPL85 |
|
Normal AM rep2
|
Alveolar macrophages from normal rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from normal rats
|
| Sample_geo_accession | GSM505316
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505316/suppl/GSM505316_CL01R002.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505316/suppl/GSM505316_CL01R002.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505317 | GPL85 |
|
Normal AM rep3
|
Alveolar macrophages from normal rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from normal rats
|
| Sample_geo_accession | GSM505317
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505317/suppl/GSM505317_CL01R003.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505317/suppl/GSM505317_CL01R003.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505318 | GPL85 |
|
Normal AM rep4
|
Alveolar macrophages from normal rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from normal rats
|
| Sample_geo_accession | GSM505318
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505318/suppl/GSM505318_CL01R004.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505318/suppl/GSM505318_CL01R004.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505319 | GPL85 |
|
Dex AM rep1
|
Alveolar macrophages from dexamethasone-treated rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from dexamethasone-treated rats
|
| Sample_geo_accession | GSM505319
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505319/suppl/GSM505319_CL01R005.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505319/suppl/GSM505319_CL01R005.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505320 | GPL85 |
|
Dex AM rep2
|
Alveolar macrophages from dexamethasone-treated rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from dexamethasone-treated rats
|
| Sample_geo_accession | GSM505320
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505320/suppl/GSM505320_CL01R006.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505320/suppl/GSM505320_CL01R006.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505321 | GPL85 |
|
Dex AM rep3
|
Alveolar macrophages from dexamethasone-treated rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from dexamethasone-treated rats
|
| Sample_geo_accession | GSM505321
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505321/suppl/GSM505321_CL01R007.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505321/suppl/GSM505321_CL01R007.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505322 | GPL85 |
|
Dex AM rep4
|
Alveolar macrophages from dexamethasone-treated rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from dexamethasone-treated rats
|
| Sample_geo_accession | GSM505322
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505322/suppl/GSM505322_CL01R008.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505322/suppl/GSM505322_CL01R008.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505323 | GPL85 |
|
Pc AM rep1
|
Alveolar macrophages from Pneumocystis carinii-infected rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from Pneumocystis carinii-infected rats
|
| Sample_geo_accession | GSM505323
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505323/suppl/GSM505323_CL01R009.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505323/suppl/GSM505323_CL01R009.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505324 | GPL85 |
|
Pc AM rep2
|
Alveolar macrophages from Pneumocystis carinii-infected rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from Pneumocystis carinii-infected rats
|
| Sample_geo_accession | GSM505324
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505324/suppl/GSM505324_CL01R010.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505324/suppl/GSM505324_CL01R010.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505325 | GPL85 |
|
Pc AM rep3
|
Alveolar macrophages from Pneumocystis carinii-infected rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from Pneumocystis carinii-infected rats
|
| Sample_geo_accession | GSM505325
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505325/suppl/GSM505325_CL01R011.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505325/suppl/GSM505325_CL01R011.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
|
GSM505326 | GPL85 |
|
Pc AM rep4
|
Alveolar macrophages from Pneumocystis carinii-infected rats
|
cell type: alveolar macrophage
|
gene expression data of alveolar macrophages from Pneumocystis carinii-infected rats
|
| Sample_geo_accession | GSM505326
| | Sample_status | Public on Apr 12 2010
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Apr 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Sprague-Dawley rats were immunosuppressed by giving dexamethasone (1.8 mg/liter) continuously in drinking water for one week and then transtracheally inoculated with 7.5 million P. carinii organisms. Eight weeks after P. carinii infection with continual immunosuppression, alveolar macrophages were isolated. Age-matched normal and dexamethasone-treated rats were used as controls.
| | Sample_growth_protocol_ch1 | Female Sprague-Dawley rats (Harlan, Indianapolis, IN) of 120-140 g were used.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from each sample using the RNeasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Chao-Hung,,Lee
| | Sample_contact_email | chlee@iupui.edu
| | Sample_contact_phone | 3172742596
| | Sample_contact_fax | 3172780643
| | Sample_contact_department | Pathology & Lab Medicine
| | Sample_contact_institute | Indiana University
| | Sample_contact_address | 1120 South Dr., FH 419
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505326/suppl/GSM505326_CL01R012.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505326/suppl/GSM505326_CL01R012.CHP.gz
| | Sample_series_id | GSE20149
| | Sample_data_row_count | 8799
| | |
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