Search results for the GEO ID: GSE20152  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM505624 | GPL1261 |
|
Ankle-hTNFa overexpression and SphK1 null-1
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: over-expression
sphingosine kinase 1 activity: null
|
Gene expression data from ankle joint of mouse with hTNFa transgene and sphingosine kinase 1 functional null; replicate 1
|
| Sample_geo_accession | GSM505624
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505624/suppl/GSM505624.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505625 | GPL1261 |
|
Ankle-hTNFa overexpression and SphK1 null-2
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: over-expression
sphingosine kinase 1 activity: null
|
Gene expression data from ankle joint of mouse with hTNFa transgene and sphingosine kinase 1 functional null; replicate 2
|
| Sample_geo_accession | GSM505625
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505625/suppl/GSM505625.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505626 | GPL1261 |
|
Ankle-Sphk1 norm-1
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: normal
sphingosine kinase 1 activity: normal
|
Gene expression data from ankle joint of mouse that are wild-type with normal sphingosine kinase 1 activity; replicate 1
|
| Sample_geo_accession | GSM505626
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505626/suppl/GSM505626.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505627 | GPL1261 |
|
Ankle-hTNFa overexpression and SphK1 null-3
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: over-expression
sphingosine kinase 1 activity: null
|
Gene expression data from ankle joint of mouse with hTNFa transgene and sphingosine kinase 1 functional null; replicate 3
|
| Sample_geo_accession | GSM505627
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505627/suppl/GSM505627.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505628 | GPL1261 |
|
Ankle-Sphk1 norm-2
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: normal
sphingosine kinase 1 activity: normal
|
Gene expression data from ankle joint of mouse that are wild-type with normal sphingosine kinase 1 activity; replicate 2
|
| Sample_geo_accession | GSM505628
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505628/suppl/GSM505628.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505629 | GPL1261 |
|
Ankle-hTNFa overexpression and Sphk1 norm-1
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: over-expression
sphingosine kinase 1 activity: normal
|
Gene expression data from ankle joint of mouse with hTNFa transgene and normal sphingosine kinase 1 activity; replicate 1
|
| Sample_geo_accession | GSM505629
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505629/suppl/GSM505629.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505630 | GPL1261 |
|
Ankle-hTNFa overexpression and Sphk1 norm-2
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: over-expression
sphingosine kinase 1 activity: normal
|
Gene expression data from ankle joint of mouse with hTNFa transgene and normal sphingosine kinase 1 activity; replicate 2
|
| Sample_geo_accession | GSM505630
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505630/suppl/GSM505630.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
|
GSM505631 | GPL1261 |
|
Ankle-hTNFa overexpression and Sphk1 norm-3
|
ankle joint from a mouse at 5 months age
|
age: 5-month adult
tnfa activity: over-expression
sphingosine kinase 1 activity: normal
|
Gene expression data from ankle joint of mouse with hTNFa transgene and normal sphingosine kinase 1 activity; replicate 3
|
| Sample_geo_accession | GSM505631
| | Sample_status | Public on Jan 15 2011
| | Sample_submission_date | Feb 02 2010
| | Sample_last_update_date | Jan 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized, euthanized, and ankle joints were removed by dissection.
| | Sample_growth_protocol_ch1 | Mice were reared and maintained using standard protocols
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).
| | Sample_hyb_protocol | Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
| | Sample_scan_protocol | Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
| | Sample_data_processing | Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | DeAnna,,Baker
| | Sample_contact_email | bakerde@musc.edu
| | Sample_contact_phone | 843-789-6798
| | Sample_contact_fax | 843-876-5131
| | Sample_contact_laboratory | Gilkeson
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Medical University of South Carolina
| | Sample_contact_address | 114 Doughty Street STB #416
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29412
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM505nnn/GSM505631/suppl/GSM505631.CEL.gz
| | Sample_series_id | GSE20152
| | Sample_data_row_count | 45101
| | |
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