Search results for the GEO ID: GSE20246  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM507410 | GPL1355 |
|
Runx2 siRNA.48h.replicate1
|
granulosa cells from PMSG primed rat ovary
|
cell type: granulosa cells from PMSG primed rat ovary
gender: female
strain: Sprague Dawley
age: 27 days
|
granulosa cells treated with forskolin and Runx2 siRNA for 48hrs
|
| Sample_geo_accession | GSM507410
| | Sample_status | Public on Feb 10 2010
| | Sample_submission_date | Feb 09 2010
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Runx2 siRNA (20 nM) or negative control siRNA (20 nM) were transfected to the cells. Transfected cells were cultured with Forskolin (10 μM) for 48 hours.
| | Sample_growth_protocol_ch1 | Granulosa cells were isolated from the ovary collected from immature rats primed with PMSG for 48 h. The cells were maintained in Opti-MEM® I Reduced Serum medium supplemented with 0.05 mg/ml of gentamycin, and 1x ITS (insulin, transferin, and selenium).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cultured granulosa cells using a RNeasy mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The total RNA was transferred to a nitrocellulose membrane and used as a template for cDNA synthesis, and biotinylated antisense cRNA probe was prepared as described by the manufacturers of the SuperScript System Kit (Invitrogen) and the ENZO BioArray HighYield RNA labeling Kit (Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | The Affymetrix Rat 230 2.0 genechip array was hybridized with the labelled probes using the standard protocol in the Affymetrix GeneChip Expression Analysis Manual
| | Sample_scan_protocol | The genechip array was scanned using a Affymetrix GCS 3000 7G scanner
| | Sample_data_processing | The signal intensity on each chip is normalized using quantile normalization algorithm of MAS5, following the Affymetrix GeneChip protocol. The overall intensity for each chip is then scaled to a target intensity of 500
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Scott,Andrew,Ochsner
| | Sample_contact_email | sochsner@bcm.edu
| | Sample_contact_phone | 713-798-6227
| | Sample_contact_laboratory | NURSA: Nuclear Receptor Signaling Atlas
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507410/suppl/GSM507410_runx2-612_062708.CEL.gz
| | Sample_series_id | GSE20246
| | Sample_data_row_count | 31099
| | |
|
GSM507411 | GPL1355 |
|
Runx2 siRNA.48h.replicate2
|
granulosa cells from PMSG primed rat ovary
|
cell type: granulosa cells from PMSG primed rat ovary
gender: female
strain: Sprague Dawley
age: 27 days
|
granulosa cells treated with forskolin and Runx2 siRNA for 48hrs
|
| Sample_geo_accession | GSM507411
| | Sample_status | Public on Feb 10 2010
| | Sample_submission_date | Feb 09 2010
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Runx2 siRNA (20 nM) or negative control siRNA (20 nM) were transfected to the cells. Transfected cells were cultured with Forskolin (10 μM) for 48 hours.
| | Sample_growth_protocol_ch1 | Granulosa cells were isolated from the ovary collected from immature rats primed with PMSG for 48 h. The cells were maintained in Opti-MEM® I Reduced Serum medium supplemented with 0.05 mg/ml of gentamycin, and 1x ITS (insulin, transferin, and selenium).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cultured granulosa cells using a RNeasy mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The total RNA was transferred to a nitrocellulose membrane and used as a template for cDNA synthesis, and biotinylated antisense cRNA probe was prepared as described by the manufacturers of the SuperScript System Kit (Invitrogen) and the ENZO BioArray HighYield RNA labeling Kit (Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | The Affymetrix Rat 230 2.0 genechip array was hybridized with the labelled probes using the standard protocol in the Affymetrix GeneChip Expression Analysis Manual
| | Sample_scan_protocol | The genechip array was scanned using a Affymetrix GCS 3000 7G scanner
| | Sample_data_processing | The signal intensity on each chip is normalized using quantile normalization algorithm of MAS5, following the Affymetrix GeneChip protocol. The overall intensity for each chip is then scaled to a target intensity of 500
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Scott,Andrew,Ochsner
| | Sample_contact_email | sochsner@bcm.edu
| | Sample_contact_phone | 713-798-6227
| | Sample_contact_laboratory | NURSA: Nuclear Receptor Signaling Atlas
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507411/suppl/GSM507411_runx2-620_062708.CEL.gz
| | Sample_series_id | GSE20246
| | Sample_data_row_count | 31099
| | |
|
GSM507412 | GPL1355 |
|
negative control siRNA.48h.replicate1
|
granulosa cells from PMSG primed rat ovary
|
cell type: granulosa cells from PMSG primed rat ovary
gender: female
strain: Sprague Dawley
age: 27 days
|
granulosa cells treated with forskolin and universal siRNA control for 48hrs
|
| Sample_geo_accession | GSM507412
| | Sample_status | Public on Feb 10 2010
| | Sample_submission_date | Feb 09 2010
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Runx2 siRNA (20 nM) or negative control siRNA (20 nM) were transfected to the cells. Transfected cells were cultured with Forskolin (10 μM) for 48 hours.
| | Sample_growth_protocol_ch1 | Granulosa cells were isolated from the ovary collected from immature rats primed with PMSG for 48 h. The cells were maintained in Opti-MEM® I Reduced Serum medium supplemented with 0.05 mg/ml of gentamycin, and 1x ITS (insulin, transferin, and selenium).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cultured granulosa cells using a RNeasy mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The total RNA was transferred to a nitrocellulose membrane and used as a template for cDNA synthesis, and biotinylated antisense cRNA probe was prepared as described by the manufacturers of the SuperScript System Kit (Invitrogen) and the ENZO BioArray HighYield RNA labeling Kit (Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | The Affymetrix Rat 230 2.0 genechip array was hybridized with the labelled probes using the standard protocol in the Affymetrix GeneChip Expression Analysis Manual
| | Sample_scan_protocol | The genechip array was scanned using a Affymetrix GCS 3000 7G scanner
| | Sample_data_processing | The signal intensity on each chip is normalized using quantile normalization algorithm of MAS5, following the Affymetrix GeneChip protocol. The overall intensity for each chip is then scaled to a target intensity of 500
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Scott,Andrew,Ochsner
| | Sample_contact_email | sochsner@bcm.edu
| | Sample_contact_phone | 713-798-6227
| | Sample_contact_laboratory | NURSA: Nuclear Receptor Signaling Atlas
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507412/suppl/GSM507412_ncin-612_062708.CEL.gz
| | Sample_series_id | GSE20246
| | Sample_data_row_count | 31099
| | |
|
GSM507413 | GPL1355 |
|
negative control siRNA.48h.replicate2
|
granulosa cells from PMSG primed rat ovary
|
cell type: granulosa cells from PMSG primed rat ovary
gender: female
strain: Sprague Dawley
age: 27 days
|
granulosa cells treated with forskolin and universal siRNA control for 48hrs
|
| Sample_geo_accession | GSM507413
| | Sample_status | Public on Feb 10 2010
| | Sample_submission_date | Feb 09 2010
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Runx2 siRNA (20 nM) or negative control siRNA (20 nM) were transfected to the cells. Transfected cells were cultured with Forskolin (10 μM) for 48 hours.
| | Sample_growth_protocol_ch1 | Granulosa cells were isolated from the ovary collected from immature rats primed with PMSG for 48 h. The cells were maintained in Opti-MEM® I Reduced Serum medium supplemented with 0.05 mg/ml of gentamycin, and 1x ITS (insulin, transferin, and selenium).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cultured granulosa cells using a RNeasy mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The total RNA was transferred to a nitrocellulose membrane and used as a template for cDNA synthesis, and biotinylated antisense cRNA probe was prepared as described by the manufacturers of the SuperScript System Kit (Invitrogen) and the ENZO BioArray HighYield RNA labeling Kit (Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | The Affymetrix Rat 230 2.0 genechip array was hybridized with the labelled probes using the standard protocol in the Affymetrix GeneChip Expression Analysis Manual
| | Sample_scan_protocol | The genechip array was scanned using a Affymetrix GCS 3000 7G scanner
| | Sample_data_processing | The signal intensity on each chip is normalized using quantile normalization algorithm of MAS5, following the Affymetrix GeneChip protocol. The overall intensity for each chip is then scaled to a target intensity of 500
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Scott,Andrew,Ochsner
| | Sample_contact_email | sochsner@bcm.edu
| | Sample_contact_phone | 713-798-6227
| | Sample_contact_laboratory | NURSA: Nuclear Receptor Signaling Atlas
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507413/suppl/GSM507413_ncin-620_062708.CEL.gz
| | Sample_series_id | GSE20246
| | Sample_data_row_count | 31099
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