Result

Search results for the GEO ID: GSE20259

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM507513

GPL1355

Sertoli cell_control_biological rep1

P20 rat Sertoli cell

cell type: Sertoli cells isolated and cultured for 72 h strain: Sprague-Dawley sex: male developmental stage: 20-days old

Gene expression data from 20-day old rat Sertoli cell cultured for 72 h as controls with no treatment

Sample_geo_accession	GSM507513
Sample_status	Public on Feb 11 2010
Sample_submission_date	Feb 10 2010
Sample_last_update_date	Feb 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	SC were plated under serum-free conditions in 24-well Falcon plates (Oxnard, CA) at 1 x 10^6 cell/well. Cells were maintained in 5% CO2 atmosphere in Ham's F-12 medium (Life Technologies) with 0.01% BSA at 32 oC for 72 h prior to collections.
Sample_growth_protocol_ch1	20 days old male rats were euthanized and testis were removed according to IACUC-approved animal use protocols. Sertoli cells (SC) were isolated as previously described (Tung, et al. 1984).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRI reagent (Sigma) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from at least 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on RAE230A array according to standard Affymetrix protocol. Chips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix Scanner 3000.
Sample_data_processing	The data were analyzed with GCOS using MAS5.0 algorithm with Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507513/suppl/GSM507513.CEL.gz
Sample_series_id	GSE20259
Sample_data_row_count	31099

GSM507514

GPL1355

Sertoli cell_control_biological rep2

P20 rat Sertoli cell

cell type: Sertoli cells isolated and cultured for 72 h strain: Sprague-Dawley sex: male developmental stage: 20-days old

Gene expression data from 20-day old rat Sertoli cell cultured for 72 h as controls with no treatment

Sample_geo_accession	GSM507514
Sample_status	Public on Feb 11 2010
Sample_submission_date	Feb 10 2010
Sample_last_update_date	Feb 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	SC were plated under serum-free conditions in 24-well Falcon plates (Oxnard, CA) at 1 x 10^6 cell/well. Cells were maintained in 5% CO2 atmosphere in Ham's F-12 medium (Life Technologies) with 0.01% BSA at 32 oC for 72 h prior to collections.
Sample_growth_protocol_ch1	20 days old male rats were euthanized and testis were removed according to IACUC-approved animal use protocols. Sertoli cells (SC) were isolated as previously described (Tung, et al. 1984).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRI reagent (Sigma) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from at least 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on RAE230A array according to standard Affymetrix protocol. Chips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix Scanner 3000.
Sample_data_processing	The data were analyzed with GCOS using MAS5.0 algorithm with Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507514/suppl/GSM507514.CEL.gz
Sample_series_id	GSE20259
Sample_data_row_count	31099

GSM507515

GPL1355

Sertoli cell_cAMP_biological rep1

P20 rat Sertoli cell

cell type: Sertoli cells isolated and cultured for 72 h strain: Sprague-Dawley sex: male developmental stage: 20-days old

Gene expression data from 20-day old rat Sertoli cell cultured for 72 h with cAMP

Sample_geo_accession	GSM507515
Sample_status	Public on Feb 11 2010
Sample_submission_date	Feb 10 2010
Sample_last_update_date	Feb 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	SC were plated under serum-free conditions in 24-well Falcon plates (Oxnard, CA) at 1 x 10^6 cell/well. Cells were maintained in 5% CO2 atmosphere in Ham's F-12 medium (Life Technologies) with 0.01% BSA at 32 oC for 72 h prior to collections.
Sample_growth_protocol_ch1	20 days old male rats were euthanized and testis were removed according to IACUC-approved animal use protocols. Sertoli cells (SC) were isolated as previously described (Tung, et al. 1984).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRI reagent (Sigma) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from at least 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on RAE230A array according to standard Affymetrix protocol. Chips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix Scanner 3000.
Sample_data_processing	The data were analyzed with GCOS using MAS5.0 algorithm with Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507515/suppl/GSM507515.CEL.gz
Sample_series_id	GSE20259
Sample_data_row_count	31099

GSM507516

GPL1355

Sertoli cell_cAMP_biological rep2

P20 rat Sertoli cell

cell type: Sertoli cells isolated and cultured for 72 h strain: Sprague-Dawley sex: male developmental stage: 20-days old

Gene expression data from 20-day old rat Sertoli cell cultured for 72 h with cAMP

Sample_geo_accession	GSM507516
Sample_status	Public on Feb 11 2010
Sample_submission_date	Feb 10 2010
Sample_last_update_date	Feb 10 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	SC were plated under serum-free conditions in 24-well Falcon plates (Oxnard, CA) at 1 x 10^6 cell/well. Cells were maintained in 5% CO2 atmosphere in Ham's F-12 medium (Life Technologies) with 0.01% BSA at 32 oC for 72 h prior to collections.
Sample_growth_protocol_ch1	20 days old male rats were euthanized and testis were removed according to IACUC-approved animal use protocols. Sertoli cells (SC) were isolated as previously described (Tung, et al. 1984).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRI reagent (Sigma) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from at least 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on RAE230A array according to standard Affymetrix protocol. Chips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix Scanner 3000.
Sample_data_processing	The data were analyzed with GCOS using MAS5.0 algorithm with Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM507nnn/GSM507516/suppl/GSM507516.CEL.gz
Sample_series_id	GSE20259
Sample_data_row_count	31099

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