Search results for the GEO ID: GSE20297  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM508815 | GPL570 |
|
HaCaT_unstimulated_1
|
HaCaT cells, unstimulated
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, unstimulated
|
| Sample_geo_accession | GSM508815
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508815/suppl/GSM508815.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508815/suppl/GSM508815.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508816 | GPL570 |
|
HaCaT_unstimulated_2
|
HaCaT cells, unstimulated
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, unstimulated
|
| Sample_geo_accession | GSM508816
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508816/suppl/GSM508816.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508816/suppl/GSM508816.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508817 | GPL570 |
|
HaCaT_unstimulated_3
|
HaCaT cells, unstimulated
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, unstimulated
|
| Sample_geo_accession | GSM508817
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508817/suppl/GSM508817.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508817/suppl/GSM508817.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508818 | GPL570 |
|
HaCaT_TNF+IFNg_1
|
HaCaT cells, stimulated with TNF-a+IFN-g for 24 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with TNF-a+IFN-g for 24 h
|
| Sample_geo_accession | GSM508818
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508818/suppl/GSM508818.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508818/suppl/GSM508818.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508819 | GPL570 |
|
HaCaT_TNF+IFNg_2
|
HaCaT cells, stimulated with TNF-a+IFN-g for 24 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with TNF-a+IFN-g for 24 h
|
| Sample_geo_accession | GSM508819
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508819/suppl/GSM508819.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508819/suppl/GSM508819.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508820 | GPL570 |
|
HaCaT_TNF+IFNg_3
|
HaCaT cells, stimulated with TNF-a+IFN-g for 24 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with TNF-a+IFN-g for 24 h
|
| Sample_geo_accession | GSM508820
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508820/suppl/GSM508820.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508820/suppl/GSM508820.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508821 | GPL570 |
|
HaCaT_TNF+IFNg+terbutaline_1
|
HaCaT cells, stimulated with terbutaline + TNF-a + IFN-g for 27 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with terbutaline + TNF-a + IFN-g for 27 h
|
| Sample_geo_accession | GSM508821
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508821/suppl/GSM508821.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508821/suppl/GSM508821.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508822 | GPL570 |
|
HaCaT_TNF+IFNg+terbutaline_2
|
HaCaT cells, stimulated with terbutaline + TNF-a + IFN-g for 27 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with terbutaline + TNF-a + IFN-g for 27 h
|
| Sample_geo_accession | GSM508822
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508822/suppl/GSM508822.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508822/suppl/GSM508822.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508823 | GPL570 |
|
HaCaT_TNF+IFNg+terbutaline_3
|
HaCaT cells, stimulated with terbutaline + TNF-a + IFN-g for 27 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with terbutaline + TNF-a + IFN-g for 27 h
|
| Sample_geo_accession | GSM508823
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508823/suppl/GSM508823.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508823/suppl/GSM508823.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508824 | GPL570 |
|
HaCaT_TNF+IFNg+GW9508_1
|
HaCaT cells, stimulated with GW9508 + TNF-a + IFN-g for 30 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with GW9508 + TNF-a + IFN-g for 30 h
|
| Sample_geo_accession | GSM508824
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508824/suppl/GSM508824.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508824/suppl/GSM508824.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
| | |
|
GSM508825 | GPL570 |
|
HaCaT_TNF+IFNg+GW9508_2
|
HaCaT cells, stimulated with GW9508 + TNF-a + IFN-g for 30 h
|
cell type: HaCaT
tissue: cell line from epidermal keratinocyte
|
HaCaT cells, stimulated with GW9508 + TNF-a + IFN-g for 30 h
|
| Sample_geo_accession | GSM508825
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508825/suppl/GSM508825.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508825/suppl/GSM508825.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
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GSM508826 | GPL570 |
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HaCaT_TNF+IFNg+GW9508_3
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HaCaT cells, stimulated with GW9508 + TNF-a + IFN-g for 30 h
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cell type: HaCaT
tissue: cell line from epidermal keratinocyte
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HaCaT cells, stimulated with GW9508 + TNF-a + IFN-g for 30 h
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| Sample_geo_accession | GSM508826
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Jun 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HaCaT cells were cultured in 96 well-plate and serum-starved for 24h. Then, terbutaline or GW9508 were added and incubated for 5min. TNF-alpha + IFN-gamma were then added and culturedfor another 24 h and cell were collected.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip 3' IVT Kit User Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000KU.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tomoko,,Fujita
| | Sample_contact_laboratory | Pharmacology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | Yoshida Konoe-Cho
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 6068501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508826/suppl/GSM508826.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM508nnn/GSM508826/suppl/GSM508826.CHP.gz
| | Sample_series_id | GSE20297
| | Sample_data_row_count | 54675
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