Search results for the GEO ID: GSE20316  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM509134 | GPL1261 |
|
mouse embryonic fibroblasts (MEF) from PTENHy/+, biological rep1
|
Primary MEFs derived from from PTENHy/+ embryos at 13.5 dpc
|
genetic background: 129/C57BL/6 mixed
genotype: PTENHy/+
cell type: mouse embryonic fibroblasts (MEF)
age: 13.5 dpc
|
Gene expression data from mouse embryonic fibroblasts (MEF) from PTENHy/+.
MEF_PTENHy+_batch1.CEL
|
| Sample_geo_accession | GSM509134
| | Sample_status | Public on Feb 15 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Feb 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | MEFs were obtained by crossing Ptenhy/+ and Pten+/- animals, embryos were harvested at 13.5 dpc, and individual MEFs were produced and cultured.
| | Sample_growth_protocol_ch1 | To preserve a constant 129/C57BL/6 mixed genetic background, we have crossed Pten hy/+ mice with Pten +/- for more than seven generations prior to analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 2.8) using PM-only models.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509134/suppl/GSM509134.CEL.gz
| | Sample_series_id | GSE20316
| | Sample_data_row_count | 45101
| | |
|
GSM509135 | GPL1261 |
|
mouse embryonic fibroblasts (MEF) from PTENHy/+, biological rep2
|
Primary MEFs derived from from PTENHy/+ embryos at 13.5 dpc
|
genetic background: 129/C57BL/6 mixed
genotype: PTENHy/+
cell type: mouse embryonic fibroblasts (MEF)
age: 13.5 dpc
|
Gene expression data from mouse embryonic fibroblasts (MEF) from PTENHy/+.
MEF_PTENHy+_batch2.CEL
|
| Sample_geo_accession | GSM509135
| | Sample_status | Public on Feb 15 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Feb 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | MEFs were obtained by crossing Ptenhy/+ and Pten+/- animals, embryos were harvested at 13.5 dpc, and individual MEFs were produced and cultured.
| | Sample_growth_protocol_ch1 | To preserve a constant 129/C57BL/6 mixed genetic background, we have crossed Pten hy/+ mice with Pten +/- for more than seven generations prior to analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 2.8) using PM-only models.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509135/suppl/GSM509135.CEL.gz
| | Sample_series_id | GSE20316
| | Sample_data_row_count | 45101
| | |
|
GSM509136 | GPL1261 |
|
mouse embryonic fibroblasts (MEF) from WT, biological rep1
|
Primary MEFs derived from from wt embryos at 13.5 dpc
|
genetic background: 129/C57BL/6 mixed
genotype: wt
cell type: mouse embryonic fibroblasts (MEF)
age: 13.5 dpc
|
Gene expression data from mouse embryonic fibroblasts (MEF) from wt.
MEF_wt_batch1.CEL
|
| Sample_geo_accession | GSM509136
| | Sample_status | Public on Feb 15 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Feb 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | MEFs were obtained by crossing Ptenhy/+ and Pten+/- animals, embryos were harvested at 13.5 dpc, and individual MEFs were produced and cultured.
| | Sample_growth_protocol_ch1 | To preserve a constant 129/C57BL/6 mixed genetic background, we have crossed Pten hy/+ mice with Pten +/- for more than seven generations prior to analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 2.8) using PM-only models.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509136/suppl/GSM509136.CEL.gz
| | Sample_series_id | GSE20316
| | Sample_data_row_count | 45101
| | |
|
GSM509137 | GPL1261 |
|
mouse embryonic fibroblasts (MEF) from WT, biological rep2
|
Primary MEFs derived from from wt embryos at 13.5 dpc
|
genetic background: 129/C57BL/6 mixed
genotype: wt
cell type: mouse embryonic fibroblasts (MEF)
age: 13.5 dpc
|
Gene expression data from mouse embryonic fibroblasts (MEF) from wt.
MEF_wt_batch2.CEL
|
| Sample_geo_accession | GSM509137
| | Sample_status | Public on Feb 15 2010
| | Sample_submission_date | Feb 12 2010
| | Sample_last_update_date | Feb 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | MEFs were obtained by crossing Ptenhy/+ and Pten+/- animals, embryos were harvested at 13.5 dpc, and individual MEFs were produced and cultured.
| | Sample_growth_protocol_ch1 | To preserve a constant 129/C57BL/6 mixed genetic background, we have crossed Pten hy/+ mice with Pten +/- for more than seven generations prior to analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 2.8) using PM-only models.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509137/suppl/GSM509137.CEL.gz
| | Sample_series_id | GSE20316
| | Sample_data_row_count | 45101
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