Search results for the GEO ID: GSE20325 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM243346 | GPL1261 |
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normal mouse kidney E12.5, biological rep 1
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normal mouse embryonic kidney day 12.5
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pool of 3 normal E12.5 kidneys
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Expression data for normal mousee embryonic day E12.5 kidney
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GSM243347 | GPL1261 |
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normal mouse kidney E12.5, biological rep 2
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normal mouse embryonic kidney day 12.5
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pool of 3 normal E12.5 kidneys
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Expression data for normal mousee embryonic day E12.5 kidney
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GSM243348 | GPL1261 |
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normal mouse kidney E12.5, biological rep 3
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normal mouse embryonic kidney day 12.5
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pool of 3 normal E12.5 kidneys
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Expression data for normal mousee embryonic day E12.5 kidney
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GSM509260 | GPL1261 |
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bcatGOF mouse kidney E12.5, biological rep 1
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mutant mouse embryonic kidney day 12.5
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genotype: ß-catenin gain-of-function mutant (ß-catGOF-UB)
tissue: kidney (uteric bud)
developmental stage: embryo
age: 12.5 days
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Hoxb7-Cre:EGFP mice ( Zhao, et al. (2004) Dev Biol 276:403-415) were crossed with mice containing loxP sites flanking exon 3 of the ß-catenin allele (ß-catdelta3/delta3) (Harada,et al. (2002) Cancer Res 62:1971-1977) to generate ß-catenin gain-of-function mutant mice specifc to the uteric bud, termed ß-catGOF-UB .Eighteen ß-catGOF-UB mutant kidneys and 9 WT kidneys were micro-dissected at E12.5. Mutant kidneys were divided into three random pools (n=3) consisting of 6 kidneys each.
Expression data for mouse ß-catGOF-UB mutant kidneys embryonic day E12.5
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Sample_geo_accession | GSM509260
| Sample_status | Public on Mar 28 2011
| Sample_submission_date | Feb 14 2010
| Sample_last_update_date | Mar 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | tissue was disected, imaged by digital microscopy and randomly sorted into one of three sample vials
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Samples were stored in RNAlater RNA stabilization reagent (Qiagen, Canada) and then isolated using the RNeasy Micro kit (Qiagen, Canada).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | one cycle amplification as per Affymetrix protocol from 1ug of total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse MOE430v2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL1261
| Sample_contact_name | Brian,Joseph,Cox
| Sample_contact_email | b.cox@utoronto.ca
| Sample_contact_laboratory | Dr. Janet Rossant
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 100 College St., Rm 13-306
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509260/suppl/GSM509260.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509260/suppl/GSM509260.CHP.gz
| Sample_series_id | GSE20325
| Sample_data_row_count | 45101
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GSM509261 | GPL1261 |
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bcatGOF mouse kidney E12.5, biological rep 2
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mutant mouse embryonic kidney day 12.5
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genotype: ß-catenin gain-of-function mutant (ß-catGOF-UB)
tissue: kidney (uteric bud)
developmental stage: embryo
age: 12.5 days
|
Hoxb7-Cre:EGFP mice ( Zhao, et al. (2004) Dev Biol 276:403-415) were crossed with mice containing loxP sites flanking exon 3 of the ß-catenin allele (ß-catdelta3/delta3) (Harada,et al. (2002) Cancer Res 62:1971-1977) to generate ß-catenin gain-of-function mutant mice specifc to the uteric bud, termed ß-catGOF-UB .Eighteen ß-catGOF-UB mutant kidneys and 9 WT kidneys were micro-dissected at E12.5. Mutant kidneys were divided into three random pools (n=3) consisting of 6 kidneys each.
Expression data for mouse ß-catGOF-UB mutant kidneys embryonic day E12.5
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Sample_geo_accession | GSM509261
| Sample_status | Public on Mar 28 2011
| Sample_submission_date | Feb 14 2010
| Sample_last_update_date | Mar 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | tissue was disected, imaged by digital microscopy and randomly sorted into one of three sample vials
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Samples were stored in RNAlater RNA stabilization reagent (Qiagen, Canada) and then isolated using the RNeasy Micro kit (Qiagen, Canada).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | one cycle amplification as per Affymetrix protocol from 1ug of total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse MOE430v2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL1261
| Sample_contact_name | Brian,Joseph,Cox
| Sample_contact_email | b.cox@utoronto.ca
| Sample_contact_laboratory | Dr. Janet Rossant
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 100 College St., Rm 13-306
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509261/suppl/GSM509261.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509261/suppl/GSM509261.CHP.gz
| Sample_series_id | GSE20325
| Sample_data_row_count | 45101
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GSM509262 | GPL1261 |
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bcatGOF mouse kidney E12.5, biological rep 3
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mutant mouse embryonic kidney day 12.5
|
genotype: ß-catenin gain-of-function mutant (ß-catGOF-UB)
tissue: kidney (uteric bud)
developmental stage: embryo
age: 12.5 days
|
Hoxb7-Cre:EGFP mice ( Zhao, et al. (2004) Dev Biol 276:403-415) were crossed with mice containing loxP sites flanking exon 3 of the ß-catenin allele (ß-catdelta3/delta3) (Harada,et al. (2002) Cancer Res 62:1971-1977) to generate ß-catenin gain-of-function mutant mice specifc to the uteric bud, termed ß-catGOF-UB .Eighteen ß-catGOF-UB mutant kidneys and 9 WT kidneys were micro-dissected at E12.5. Mutant kidneys were divided into three random pools (n=3) consisting of 6 kidneys each.
Expression data for mouse ß-catGOF-UB mutant kidneys embryonic day E12.5
|
Sample_geo_accession | GSM509262
| Sample_status | Public on Mar 28 2011
| Sample_submission_date | Feb 14 2010
| Sample_last_update_date | Mar 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | tissue was disected, imaged by digital microscopy and randomly sorted into one of three sample vials
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Samples were stored in RNAlater RNA stabilization reagent (Qiagen, Canada) and then isolated using the RNeasy Micro kit (Qiagen, Canada).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | one cycle amplification as per Affymetrix protocol from 1ug of total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse MOE430v2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL1261
| Sample_contact_name | Brian,Joseph,Cox
| Sample_contact_email | b.cox@utoronto.ca
| Sample_contact_laboratory | Dr. Janet Rossant
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 100 College St., Rm 13-306
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509262/suppl/GSM509262.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM509nnn/GSM509262/suppl/GSM509262.CHP.gz
| Sample_series_id | GSE20325
| Sample_data_row_count | 45101
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