Search results for the GEO ID: GSE20505  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM515232 | GPL570 |
|
Donor 1, 0 h
|
Bone marrow donor 1, CD34+ cells before stimulation
|
cell type: bone marrow-derived CD34+ cells
treatment: none
treatment time: 0 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells before stimulation.
Don 1 0 h
|
| Sample_geo_accession | GSM515232
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515232/suppl/GSM515232.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515233 | GPL570 |
|
Donor 1, S1 stimulation, 48 h
|
Bone marrow donor 1, CD34+ cells stimulated in S1 after 48 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 48 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells after stimulation with S1 for 48 h.
Don 1 S1-48
|
| Sample_geo_accession | GSM515233
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515233/suppl/GSM515233.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515234 | GPL570 |
|
Donor 1, S2 stimulation, 48 h
|
Bone marrow donor 1, CD34+ cells stimulated in S2 after 48 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 48 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells after stimulation with S2 for 48 h.
Don 1 S2-48
|
| Sample_geo_accession | GSM515234
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515234/suppl/GSM515234.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515235 | GPL570 |
|
Donor 1, S1 stimulation, 72 h
|
Bone marrow donor 1, CD34+ cells stimulated in S1 after 72 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 72 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells after stimulation with S1 for 72 h.
Don 1 S1-72
|
| Sample_geo_accession | GSM515235
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515235/suppl/GSM515235.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515236 | GPL570 |
|
Donor 1, S2 stimulation, 72 h
|
Bone marrow donor 1, CD34+ cells stimulated in S2 after 72 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 72 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells after stimulation with S2 for 72 h.
Don 1 S2-72
|
| Sample_geo_accession | GSM515236
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515236/suppl/GSM515236.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515237 | GPL570 |
|
Donor 1, S1 stimulation, 96 h
|
Bone marrow donor 1, CD34+ cells stimulated in S1 after 96 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 96 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells after stimulation with S1 for 96 h.
Don 1 S1-96
|
| Sample_geo_accession | GSM515237
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515237/suppl/GSM515237.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515238 | GPL570 |
|
Donor 1, S2 stimulation, 96 h
|
Bone marrow donor 1, CD34+ cells stimulated in S2 after 96 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 96 hr
donor: 1
|
Gene expression data from donor 1 human CD34+ bone marrow-derived cells after stimulation with S2 for 96 h.
Don 1 S2-96
|
| Sample_geo_accession | GSM515238
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515238/suppl/GSM515238.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515239 | GPL570 |
|
Donor 2, 0 h
|
Bone marrow donor 2, CD34+ cells before stimulation
|
cell type: bone marrow-derived CD34+ cells
treatment: none
treatment time: 0 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells before stimulation.
Don 2 0 h
|
| Sample_geo_accession | GSM515239
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515239/suppl/GSM515239.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515240 | GPL570 |
|
Donor 2, S1 stimulation, 48 h
|
Bone marrow donor 2, CD34+ cells stimulated in S1 after 48 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 48 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells after stimulation with S1 for 48 h.
Don 2 S1-48
|
| Sample_geo_accession | GSM515240
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515240/suppl/GSM515240.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515241 | GPL570 |
|
Donor 2, S2 stimulation, 48 h
|
Bone marrow donor 2, CD34+ cells stimulated in S2 after 48 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 48 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells after stimulation with S2 for 48 h.
Don 2 S2-48
|
| Sample_geo_accession | GSM515241
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515241/suppl/GSM515241.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515242 | GPL570 |
|
Donor 2, S1 stimulation, 72 h
|
Bone marrow donor 2, CD34+ cells stimulated in S1 after 72 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 72 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells after stimulation with S1 for 72 h.
Don 2 S1-72
|
| Sample_geo_accession | GSM515242
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515242/suppl/GSM515242.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515243 | GPL570 |
|
Donor 2, S2 stimulation, 72 h
|
Bone marrow donor 2, CD34+ cells stimulated in S2 after 72 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 72 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells after stimulation with S2 for 72 h.
Don 2 S2-72
|
| Sample_geo_accession | GSM515243
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515243/suppl/GSM515243.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515244 | GPL570 |
|
Donor 2, S1 stimulation, 96 h
|
Bone marrow donor 2, CD34+ cells stimulated in S1 after 96 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 96 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells after stimulation with S1 for 96 h.
Don 2 S1-96
|
| Sample_geo_accession | GSM515244
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515244/suppl/GSM515244.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515245 | GPL570 |
|
Donor 2, S2 stimulation, 96 h
|
Bone marrow donor 2, CD34+ cells stimulated in S2 after 96 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 96 hr
donor: 2
|
Gene expression data from donor 2 human CD34+ bone marrow-derived cells after stimulation with S2 for 96 h.
Don 2 S2-96
|
| Sample_geo_accession | GSM515245
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515245/suppl/GSM515245.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515246 | GPL570 |
|
Donor 3, 0 h
|
Bone marrow donor 3, CD34+ cells before stimulation
|
cell type: bone marrow-derived CD34+ cells
treatment: none
treatment time: 0 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells before stimulation.
Don 3 0 h
|
| Sample_geo_accession | GSM515246
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515246/suppl/GSM515246.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515247 | GPL570 |
|
Donor 3, S1 stimulation, 48 h
|
Bone marrow donor 3, CD34+ cells stimulated in S1 after 48 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 48 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells after stimulation with S1 for 48 h.
Don 3 S1-48
|
| Sample_geo_accession | GSM515247
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515247/suppl/GSM515247.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515248 | GPL570 |
|
Donor 3, S2 stimulation, 48 h
|
Bone marrow donor 3, CD34+ cells stimulated in S2 after 48 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 48 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells after stimulation with S2 for 48 h.
Don 3 S2-48
|
| Sample_geo_accession | GSM515248
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515248/suppl/GSM515248.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515249 | GPL570 |
|
Donor 3, S1 stimulation, 72 h
|
Bone marrow donor 3, CD34+ cells stimulated in S1 after 72 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 72 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells after stimulation with S1 for 72 h.
Don 3 S1-72
|
| Sample_geo_accession | GSM515249
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515249/suppl/GSM515249.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515250 | GPL570 |
|
Donor 3, S2 stimulation, 72 h
|
Bone marrow donor 3, CD34+ cells stimulated in S2 after 72 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 72 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells after stimulation with S2 for 72 h.
Don 3 S2-72
|
| Sample_geo_accession | GSM515250
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515250/suppl/GSM515250.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515251 | GPL570 |
|
Donor 3, S1 stimulation, 96 h
|
Bone marrow donor 3, CD34+ cells stimulated in S1 after 96 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S1 stimulation (French trial conditions)
treatment time: 96 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells after stimulation with S1 for 96 h.
Don 3 S1-96
|
| Sample_geo_accession | GSM515251
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515251/suppl/GSM515251.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
GSM515252 | GPL570 |
|
Donor 3, S2 stimulation, 96 h
|
Bone marrow donor 3, CD34+ cells stimulated in S2 after 96 h
|
cell type: bone marrow-derived CD34+ cells
treatment: S2 stimulation (British trial conditions)
treatment time: 96 hr
donor: 3
|
Gene expression data from donor 3 human CD34+ bone marrow-derived cells after stimulation with S2 for 96 h.
Don 3 S2-96
|
| Sample_geo_accession | GSM515252
| | Sample_status | Public on Feb 24 2010
| | Sample_submission_date | Feb 23 2010
| | Sample_last_update_date | Feb 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were stimulated according to S1 or S2 conditions. Cells from each time point (0 h, 48 h, 72 h, 96 h) were collected and washed with PBS.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S1 conditions: X-vivo 10, 4% fetal calf serum, 300 ng/ml SCF, 100 ng/ml TPO, 60 ng/ml IL3, and 300 ng/ml FL.
| | Sample_treatment_protocol_ch1 |
| | Sample_treatment_protocol_ch1 | S2 conditions: X-vivo 10, 1% human serum albumin, 300 ng/ml SCF, 100 ng/ml TPO, 20 ng/ml IL3, and 300 ng/ml FL.
| | Sample_growth_protocol_ch1 | Bone marrow-derived CD34+ cells were isolated from three human donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Picopure RNA Isolation kit (Molecular Devices, Sunnyvale, CA) from unstimulated cells and from cells cultured in S1 or S2 conditions for 48, 72, or 96 h. Quality of RNA was assessed by Bioanalyzer (Agilent, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One-hundred ng of total RNA was subjected to one round amplification using the Riboamp RNA amplification kit (Molecular Devices) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Fifteen micrograms of fragmented, biotinylated cRNA was hybridized to HG U133 Plus 2.0 microarrays (Affymetrix) for 16 hours at 45 deg C. Genechips were hybridized, washed, and stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Microarray chips were scanned with a 532-nm emission laser and underwent detection at 570 nm.
| | Sample_data_processing | GeneChip images were quantified using GeneChip Operating Software (GCOS, Affymetrix) and normalized by RMA (Genesifter.net).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amrita,,Ghosh
| | Sample_contact_laboratory | Disorders of Immunity
| | Sample_contact_department | Genetics and Molecular Biology
| | Sample_contact_institute | National Human Genome Research Institute (NHGRI), NIH
| | Sample_contact_address | 49 Convent Drive, 3B-07
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM515nnn/GSM515252/suppl/GSM515252.CEL.gz
| | Sample_series_id | GSE20505
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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