Search results for the GEO ID: GSE20611  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM517854 | GPL96 |
|
MDA-MB-231_parental_replicate_1
|
Parental cell line of SCP6
|
cell line: MDA-MB-231
cell derivative: breast cancer
|
Parental cell line of SCP6.
Parental-1
|
| Sample_geo_accession | GSM517854
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517854/suppl/GSM517854.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517854/suppl/GSM517854.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517855 | GPL96 |
|
MDA-MB-231_parental_replicate_2
|
Parental cell line of SCP6
|
cell line: MDA-MB-231
cell derivative: breast cancer
|
Parental cell line of SCP6.
Parental-2
|
| Sample_geo_accession | GSM517855
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517855/suppl/GSM517855.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517855/suppl/GSM517855.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517856 | GPL96 |
|
Post-dormancy_PD1
|
Highly metastatic derivative of SCP6
|
cell line: PD1
cell derivative: breast cancer
|
Highly metastatic derivative of SCP6.
PD1
|
| Sample_geo_accession | GSM517856
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517856/suppl/GSM517856.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517856/suppl/GSM517856.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517857 | GPL96 |
|
Post-dormancy_PD2A
|
Highly metastatic derivative of PD1
|
cell line: PD2A
cell derivative: breast cancer
|
Highly metastatic derivative of PD1.
PD2A
|
| Sample_geo_accession | GSM517857
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517857/suppl/GSM517857.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517857/suppl/GSM517857.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517858 | GPL96 |
|
Post-dormancy_PD2B
|
Highly metastatic derivative of PD1
|
cell line: PD2B
cell derivative: breast cancer
|
Highly metastatic derivative of PD1.
PD2B
|
| Sample_geo_accession | GSM517858
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517858/suppl/GSM517858.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517858/suppl/GSM517858.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517859 | GPL96 |
|
Post-dormancy_PD2C
|
Highly metastatic derivative of PD1
|
cell line: PD2C
cell derivative: breast cancer
|
Highly metastatic derivative of PD1.
PD2C
|
| Sample_geo_accession | GSM517859
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517859/suppl/GSM517859.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517859/suppl/GSM517859.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517860 | GPL96 |
|
Post-dormancy_PD2D
|
Highly metastatic derivative of PD1
|
cell line: PD2D
cell derivative: breast cancer
|
Highly metastatic derivative of PD1.
PD2D
|
| Sample_geo_accession | GSM517860
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517860/suppl/GSM517860.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517860/suppl/GSM517860.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517861 | GPL96 |
|
Post-dormancy_PD2E
|
Highly metastatic derivative of PD1
|
cell line: PD2E
cell derivative: breast cancer
|
Highly metastatic derivative of PD1.
PD2E
|
| Sample_geo_accession | GSM517861
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517861/suppl/GSM517861.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517861/suppl/GSM517861.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517862 | GPL96 |
|
Post-dormancy_PD2R
|
Mildly metastatic derivative of PD1
|
cell line: PD2R
cell derivative: breast cancer
|
Mildly metastatic derivative of PD1.
PD2R
|
| Sample_geo_accession | GSM517862
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517862/suppl/GSM517862.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517862/suppl/GSM517862.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517863 | GPL96 |
|
MDA-MB-231_variant_SCP3
|
Weakly metastatic derivative of MDA-MB-231
|
cell line: SCP3
cell derivative: breast cancer
|
Weakly metastatic derivative of MDA-MB-231.
SCP3
|
| Sample_geo_accession | GSM517863
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517863/suppl/GSM517863.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517863/suppl/GSM517863.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
| | |
|
GSM517864 | GPL96 |
|
MDA-MB-231_variant_SCP4
|
Weakly metastatic derivative of MDA-MB-231
|
cell line: SCP4
cell derivative: breast cancer
|
Weakly metastatic derivative of MDA-MB-231.
SCP4
|
| Sample_geo_accession | GSM517864
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517864/suppl/GSM517864.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517864/suppl/GSM517864.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
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GSM517865 | GPL96 |
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MDA-MB-231_variant_SCP6
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Weakly metastatic derivative of MDA-MB-231
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cell line: SCP6
cell derivative: breast cancer
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Weakly metastatic derivative of MDA-MB-231.
SCP6
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| Sample_geo_accession | GSM517865
| | Sample_status | Public on Jan 30 2011
| | Sample_submission_date | Mar 03 2010
| | Sample_last_update_date | Jan 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown to sub-confluency before harvesting RNA.
| | Sample_growth_protocol_ch1 | Cell lines were maintained in DMEM with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using Qiagen RNAeasy mini kit following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133A Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Genespring GX 7.3 Expression Analysis using Per Chip (normalize to 50th percentile) and Per Gene (normalize to median) normalization methods.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Yibin,,Kang
| | Sample_contact_email | ykang@princeton.edu
| | Sample_contact_phone | 609-258-9120
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Princeton University
| | Sample_contact_address | LTL 254, Washington Road
| | Sample_contact_city | Princeton
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 08544
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517865/suppl/GSM517865.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517865/suppl/GSM517865.CHP.gz
| | Sample_series_id | GSE20611
| | Sample_data_row_count | 22283
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