Search results for the GEO ID: GSE20615 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM517901 | GPL570 |
|
HEK293T cell culture,control,biological rep 1
|
HEK293T human embryonic epithelial kidney cell culture,control,biological rep 1
|
cell line: HEK293T
|
CTR_1
|
Sample_geo_accession | GSM517901
| Sample_status | Public on Oct 05 2011
| Sample_submission_date | Mar 03 2010
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517901/suppl/GSM517901.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM517902 | GPL570 |
|
HEK293T cell culture,control,biological rep 2
|
HEK293T human embryonic epithelial kidney cell culture,control,biological rep 2
|
cell line: HEK293T
|
CTR_2
|
Sample_geo_accession | GSM517902
| Sample_status | Public on Oct 05 2011
| Sample_submission_date | Mar 03 2010
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517902/suppl/GSM517902.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM517903 | GPL570 |
|
HEK293T cell culture,control,biological rep 3
|
HEK293T human embryonic epithelial kidney cell culture,control,biological rep 3
|
cell line: HEK293T
|
CTR_3
|
Sample_geo_accession | GSM517903
| Sample_status | Public on Oct 05 2011
| Sample_submission_date | Mar 03 2010
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517903/suppl/GSM517903.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM517904 | GPL570 |
|
HEK293T cell culture,transfected,biological rep 1
|
HEK293T human embryonic epithelial kidney cell culture,transfected,biological rep 1
|
cell line: HEK293T
|
GFP_1
|
Sample_geo_accession | GSM517904
| Sample_status | Public on Oct 05 2011
| Sample_submission_date | Mar 03 2010
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517904/suppl/GSM517904.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM517905 | GPL570 |
|
HEK293T cell culture,transfected,biological rep 2
|
HEK293T human embryonic epithelial kidney cell culture,transfected,biological rep 2
|
cell line: HEK293T
|
GFP_2
|
Sample_geo_accession | GSM517905
| Sample_status | Public on Oct 05 2011
| Sample_submission_date | Mar 03 2010
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517905/suppl/GSM517905.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM517906 | GPL570 |
|
HEK293T cell culture,transfected,biological rep 3
|
HEK293T human embryonic epithelial kidney cell culture,transfected,biological rep 3
|
cell line: HEK293T
|
GFP_3
|
Sample_geo_accession | GSM517906
| Sample_status | Public on Oct 05 2011
| Sample_submission_date | Mar 03 2010
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM517nnn/GSM517906/suppl/GSM517906.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039977 | GPL570 |
|
HEK293T cell culture,control,biological rep 4
|
HEK293T human embryonic epithelial kidney cell culture,control,biological rep 4
|
cell line: HEK293T
|
CTR_4
|
Sample_geo_accession | GSM1039977
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039977/suppl/GSM1039977_AnP_Hu13302_062910_03_CTR26.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039978 | GPL570 |
|
HEK293T cell culture,control,biological rep 5
|
HEK293T human embryonic epithelial kidney cell culture,control,biological rep 5
|
cell line: HEK293T
|
CTR_5
|
Sample_geo_accession | GSM1039978
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039978/suppl/GSM1039978_AnP_Hu13302_062910_06_CTR27.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039979 | GPL570 |
|
HEK293T cell culture,control,biological rep 6
|
HEK293T human embryonic epithelial kidney cell culture,control,biological rep 6
|
cell line: HEK293T
|
CTR_6
|
Sample_geo_accession | GSM1039979
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039979/suppl/GSM1039979_AnP_Hu13302_062910_09_CTR29.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039980 | GPL570 |
|
HEK293T cell culture,transfected,biological rep 4
|
HEK293T human embryonic epithelial kidney cell culture,transfected,biological rep 4
|
cell line: HEK293T
|
GFP_4
|
Sample_geo_accession | GSM1039980
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039980/suppl/GSM1039980_AnP_Hu13302_062910_01_P26.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039981 | GPL570 |
|
HEK293T cell culture,transfected,biological rep 5
|
HEK293T human embryonic epithelial kidney cell culture,transfected,biological rep 5
|
cell line: HEK293T
|
GFP_5
|
Sample_geo_accession | GSM1039981
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039981/suppl/GSM1039981_AnP_Hu13302_062910_04_P27.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039982 | GPL570 |
|
HEK293T cell culture,transfected,biological rep 6
|
HEK293T human embryonic epithelial kidney cell culture,transfected,biological rep 6
|
cell line: HEK293T
|
GFP_6
|
Sample_geo_accession | GSM1039982
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039982/suppl/GSM1039982_AnP_Hu13302_062910_07_P29.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039983 | GPL570 |
|
HEK293T cell culture,untransfected,biological rep 1
|
HEK293T human embryonic epithelial kidney cell culture,untransfected,biological rep 1
|
cell line: HEK293T
|
GFPMINUS_1
|
Sample_geo_accession | GSM1039983
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039983/suppl/GSM1039983_AnP_Hu13302_062910_02_M26.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039984 | GPL570 |
|
HEK293T cell culture,untransfected,biological rep 2
|
HEK293T human embryonic epithelial kidney cell culture,untransfected,biological rep 2
|
cell line: HEK293T
|
GFPMINUS_2
|
Sample_geo_accession | GSM1039984
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039984/suppl/GSM1039984_AnP_Hu13302_062910_05_M27.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
|
GSM1039985 | GPL570 |
|
HEK293T cell culture,untransfected,biological rep 3
|
HEK293T human embryonic epithelial kidney cell culture,untransfected,biological rep 3
|
cell line: HEK293T
|
GFPMINUS_3
|
Sample_geo_accession | GSM1039985
| Sample_status | Public on Nov 21 2012
| Sample_submission_date | Nov 21 2012
| Sample_last_update_date | Nov 21 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's (Invitrogen) instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| Sample_data_processing | The data was analysed using R 2.9.0 with Bioconductor 2.4. Arrays were normalised via RMA, and all treatments were compared to the control LA via the limma package. Benjamini-Hochberg adjusted p-values were used as significance measurement.
| Sample_platform_id | GPL570
| Sample_contact_name | Jean-Jack,M.,Riethoven
| Sample_contact_email | jeanjack@unl.edu
| Sample_contact_laboratory | Bioinformatics Core Research Facility
| Sample_contact_department | Beadle Center for Biotechnology
| Sample_contact_institute | University of Nebraska-Lincoln
| Sample_contact_address | 1901 Vine Street
| Sample_contact_city | Lincoln
| Sample_contact_state | NE
| Sample_contact_zip/postal_code | 68588-0665
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1039nnn/GSM1039985/suppl/GSM1039985_AnP_Hu13302_062910_08_M29.CEL.gz
| Sample_series_id | GSE20615
| Sample_data_row_count | 54675
| |
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