Result

Search results for the GEO ID: GSE20645

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM518339

GPL1261

OPC-31.5 ℃ -rep1

OPC cultured at 31.5 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 31.5 ℃

Sample_geo_accession	GSM518339
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518339/suppl/GSM518339.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518340

GPL1261

OPC-37 ℃ -rep1

OPC cultured at 37 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 37 ℃

Sample_geo_accession	GSM518340
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518340/suppl/GSM518340.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518341

GPL1261

OPC-31.5 ℃ -rep2

OPC cultured at 31.5 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 31.5 ℃

Sample_geo_accession	GSM518341
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518341/suppl/GSM518341.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518342

GPL1261

OPC-37 ℃ -rep2

OPC cultured at 37 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 37 ℃

Sample_geo_accession	GSM518342
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518342/suppl/GSM518342.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518343

GPL1261

OPC-31.5 ℃ -rep3

OPC cultured at 31.5 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 31.5 ℃

Sample_geo_accession	GSM518343
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518343/suppl/GSM518343.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518344

GPL1261

OPC-37 ℃ -rep3

OPC cultured at 37 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 37 ℃

Sample_geo_accession	GSM518344
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518344/suppl/GSM518344.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518345

GPL1261

OPC-31.5 ℃ -rep4

OPC cultured at 31.5 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 31.5 ℃

Sample_geo_accession	GSM518345
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518345/suppl/GSM518345.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

GSM518346

GPL1261

OPC-37 ℃ -rep4

OPC cultured at 37 ℃

strain: ICR cell type: oligodendrocyte precursor cells temperature: 37 ℃

Sample_geo_accession	GSM518346
Sample_status	Public on Mar 06 2010
Sample_submission_date	Mar 05 2010
Sample_last_update_date	Mar 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were passaged to serum-free basal medium and cultured for further 48 hr at 37 ℃ and 31.5 ℃.
Sample_growth_protocol_ch1	Oligodendrocyte precursor cells were prepared from primary mixed cell cultures of embryonic mouse cerebral hemispheres. The cerebral hemispheres from 15-day-old mouse embryos were enzymatically dissociated, seeded and cultured for 5 days in D-MEM containing 10% FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Total RNA was extracted from mice (n	3 per each group) using TRIzol (Life Technologies, Rockville, TX) and re-purified by RNeasy spin columns (Quiagen, Valencia, CA), according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The labeled cRNA was prepared from by in vitro transcription (Enzo Biochem, New York, NY)
Sample_hyb_protocol	The labeled cRNA was fragmented, hybridized to Mouse430 2.0 array (Affymetrix, Santa Clara, CA) using an Affymetrix fluidics station according to the Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned with an Affymetrix scanner, according to the Affymetrix protocols.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Masahiro,,Yamamoto
Sample_contact_email	hirokoma@h.email.ne.jp
Sample_contact_department	Department of Kampo Medicine
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinano-machi
Sample_contact_city	Shinjuku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518346/suppl/GSM518346.CEL.gz
Sample_series_id	GSE20645
Sample_data_row_count	45101

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