Search results for the GEO ID: GSE20667  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM518494 | GPL570 |
|
T-ALL1 Control 1
|
control peptide-treated T-ALL1 100mM 16h
|
cell line: JCRB0086 [TALL-1]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM518494
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | Mar 07 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518494/suppl/GSM518494.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM518495 | GPL570 |
|
T-ALL1 Control 2
|
control peptide-treated T-ALL1 100mM 16h
|
cell line: JCRB0086 [TALL-1]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM518495
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | Mar 07 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518495/suppl/GSM518495.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM518496 | GPL570 |
|
T-ALL1 NBD 1
|
NBD inhibitory peptide-treated T-ALL1 100mM 16h
|
cell line: JCRB0086 [TALL-1]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM518496
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | Mar 07 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518496/suppl/GSM518496.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM518497 | GPL570 |
|
T-ALL1 NBD 2
|
NBD inhibitory peptide-treated T-ALL1 100mM 16h
|
cell line: JCRB0086 [TALL-1]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM518497
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | Mar 07 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM518nnn/GSM518497/suppl/GSM518497.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542393 | GPL570 |
|
CEM Control 1
|
control peptide-treated CEM 100mM 16h
|
cell line: JCRB0033 [CEM]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542393
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542393/suppl/GSM542393.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542394 | GPL570 |
|
CEM Control 2
|
control peptide-treated CEM 100mM 16h
|
cell line: JCRB0033 [CEM]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542394
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542394/suppl/GSM542394.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542395 | GPL570 |
|
CEM NBD 1
|
NBD inhibitory peptide-treated CEM 100mM 16h
|
cell line: JCRB0033 [CEM]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542395
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542395/suppl/GSM542395.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542396 | GPL570 |
|
CEM NBD 2
|
NBD inhibitory peptide-treated CEM 100mM 16h
|
cell line: JCRB0033 [CEM]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542396
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542396/suppl/GSM542396.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542397 | GPL570 |
|
KOPT-K control 1
|
control peptide-treated KOPT-K 100mM 16h
|
cell line: KOPT-K
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542397
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542397/suppl/GSM542397.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542398 | GPL570 |
|
KOPT-K control 2
|
control peptide-treated KOPT-K 100mM 16h
|
cell line: KOPT-K
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542398
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542398/suppl/GSM542398.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542399 | GPL570 |
|
KOPT-K NBD 1
|
NBD inhibitory peptide-treated KOPT-K 100mM 16h
|
cell line: KOPT-K
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542399
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542399/suppl/GSM542399.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542400 | GPL570 |
|
KOPT-K NBD 2
|
NBD inhibitory peptide-treated KOPT-K 100mM 16h
|
cell line: KOPT-K
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542400
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542400/suppl/GSM542400.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542401 | GPL570 |
|
DND41 Control 1
|
control peptide-treated DND41 100mM 16h
|
cell line: ACC525 [DND41]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542401
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542401/suppl/GSM542401.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542402 | GPL570 |
|
DND41 Control 2
|
control peptide-treated DND41 100mM 16h
|
cell line: ACC525 [DND41]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542402
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542402/suppl/GSM542402.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542403 | GPL570 |
|
DND41 NBD 1
|
NBD inhibitory peptide-treated DND41 100mM 16h
|
cell line: ACC525 [DND41]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542403
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542403/suppl/GSM542403.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542404 | GPL570 |
|
DND41 NBD 2
|
NBD inhibitory peptide-treated DND41 100mM 16h
|
cell line: ACC525 [DND41]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542404
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542404/suppl/GSM542404.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542405 | GPL570 |
|
HPB-ALL Control 1
|
control peptide-treated HPB-ALL 100mM 16h
|
cell line: ACC483 [HPB-ALL]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542405
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542405/suppl/GSM542405.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542406 | GPL570 |
|
HPB-ALL Control 2
|
control peptide-treated HPB-ALL 100mM 16h
|
cell line: ACC483 [HPB-ALL]
cell type: human T-ALL cell line
agent: control
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542406
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542406/suppl/GSM542406.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
| | |
|
GSM542407 | GPL570 |
|
HPB-ALL NBD 1
|
NBD inhibitory peptide-treated HPB-ALL 100mM 16h
|
cell line: ACC483 [HPB-ALL]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
|
Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
|
| Sample_geo_accession | GSM542407
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542407/suppl/GSM542407.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
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GSM542408 | GPL570 |
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HPB-ALL NBD 2
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NBD inhibitory peptide-treated HPB-ALL 100mM 16h
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cell line: ACC483 [HPB-ALL]
cell type: human T-ALL cell line
agent: IKKγ Nemo binding domain (NBD) inhibitory peptide
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Twenty samples were analyzed: human T-ALL, CEM, KOPT-K, DND41, HPB-ALL cells lines have been treated at 100uM for 16 hours with control peptide or IKKγ Nemo binding domain (NBD) inhibitory peptide, that specifically block the canonical NF-κB activity by disrupting the interaction of IKKγ to IKKβ and IKKα
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| Sample_geo_accession | GSM542408
| | Sample_status | Public on May 07 2011
| | Sample_submission_date | May 10 2010
| | Sample_last_update_date | May 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | T-ALL1, CEM, KOPT-K, DND41 and HPB-ALL cells lines have been treated with control peptide or IKKg NEMO binding domain (NDB) inhibitory peptide at 100mM for 16 hours
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy (QIAGEN) according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 20 ng total RNA converted to cDNA using Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| | Sample_hyb_protocol | 15 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Iannis,,Aifantis
| | Sample_contact_email | iannis.aifantis@med.nyu.edu
| | Sample_contact_phone | (212) 263 5365
| | Sample_contact_laboratory | MSB 538
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU Sch of Medicine
| | Sample_contact_address | 550 First Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM542nnn/GSM542408/suppl/GSM542408.CEL.gz
| | Sample_series_id | GSE20667
| | Sample_data_row_count | 54675
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